BMP4调控睾丸支持细胞增殖的初步研究

doi:10.11843/j.issn.0366-6964.2021.04.014

Abstract

Abstract: The study aimed to explore the the regulation of BMP4 on the proliferation of testicular sertoli cells. Three healthy Dazu black goat rams in the 0-, 1-, 2- and 3-month-old groups were selected respectively, and the testicular sertoli cells were collected. Each experiment set up 3 biological replicates and 3 technical replicates. Whether BMP4 regulated goat testicular sertoli cells through Id2 and their regulatory relationship were verified by in vitro culture, cell immunofluorescence, gene interference, overexpression, qPCR and Western blotting and other technologies. The results showed that the expression level of BMP4 in testicular sertoli cells of Dazu black goat in 2-month-old group was extremely significantly higher than that in 0-,1-month-old groups (P<0.01); Within a certain range, with the increase of BMP4 concentration, the proliferation activity of testicular sertoli cells increased, and the proliferation ability was the strongest when the concentration of BMP4 was 200 ng·mL^-1(P<0.05); After interfering BMP4 expression, the cell proliferation activity significantly decreased (P<0.05), but returned to the normal level after 72 h (P>0.05), and the expression of PCNA was extremely significantly reduced (P<0.01), indicating that the cell proliferation ability was restricted after interfering BMP4 expression, and the cell proliferation index measurement results further illustrated that the cell proliferation ability was restricted after interfering BMP4 expression; After BMP4 overexpression, the expression levels of Id2 extremely significantly increased (P<0.01), indicating that BMP4 had a positive regulatory effect on Id2; Compared to the overexpressing BMP4 and then interfering Id2 group, in the interfering Id2 group, the expression levels of PCNA gene significantly reduced (P<0.05), which further proved BMP4 could regulate the expression of this gene and protein. In summary, the cell proliferation activity of goat testicular sertoli cells is positively correlated with the concentration of BMP4 within a certain range; BMP4 can positively regulate the expression of Id2, and promote the proliferation of goat testicular sertoli cells through promoting the expression of Id2. This study provides a basis for elucidating the molecular mechanism and physiological functions of BMP4 regulating goat testicular sertoli cells.

Key words: goat, sertoli cells, BMP4, Id2, proliferation

CLC Number:

S827.3

HUANG Siyi, QIAO Lei, HE Lina, WANG Lei, LI Bijun, CHEN Juncai, ZHAO Zhongquan. Preliminary Study on BMP4 Regulating Proliferation of Testicular Sertoli Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 987-995.

References 32

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Preliminary Study on BMP4 Regulating Proliferation of Testicular Sertoli Cells

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