藏猪源捷申病毒的检测和遗传演化分析

doi:10.11843/j.issn.0366-6964.2021.019

Abstract

Abstract: In this study, we investigated the prevalence, molecular characteristics, and genetic evolution of porcine teschovirus (PTV) in Tibetan pigs in Sichuan. Ninety-six fecal samples of Tibetan pigs were collected from 14 farms in Kangding region of Ganzi Tibetan Autonomous Prefecture, Sichuan Province from 2017 to 2018. The samples were detected by the RT-PCR method, and the viral genome was sequenced and genotyped. The overall positive rate of PTV was 20.8% (20/96, 95% CI=13.2%-30.3%), and the positive rate of PTV was 57.1% (8/14, 95% CI=28.9%-82.3%) in 14 pig farms. Two nearly full-length PTV complete genome sequences were obtained from 2 different clinical samples. The two genomes were of 83.1% nucleotide identity and were named as SWU-E5-2018 and SWU-ZG2-2018. The serotypes of the two sequences were found to be type 3 and type 6 by VP1 sequence analysis. Furthermore, the SWU-E5-2018 was found to be a recombinant strain by recombination analysis, and the recombinant region was located at 552-3 082 nt of capsid protein P1. To have a further understanding of the evolution process of the two PTV sequences, the divergence time estimation was performed using the Bayesian evolutionary analysis software. The result showed that the divergence timepoints of SWU-E5-2018 and SWU-ZG2-2018 was about 2010 and 2011, respectively. It was the first time to investigate the PTV from Tibetan pigs and to confirm the existence and prevalence of PTV in Tibetan pigs, which provided a reference for further study of viral genetic variation and biological characteristics.

Key words: Tibetan pig, porcine teschovirus, genome, recombination analysis, molecular clock

CLC Number:

S852.659.6

WANG Lixuan, ZHOU Qun, FU Nengsheng, CAO Hui, HE Xinyi, FANG Pengfei, HU Chengzhe, ZHANG Bin. Detection and Genetic Evolution of Teschovirus of Tibetan Pig Origin in Sichuan[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 185-194.

References 25

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Detection and Genetic Evolution of Teschovirus of Tibetan Pig Origin in Sichuan

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