鸡弱精症相关精浆蛋白组分析

doi:10.11843/j.issn.0366-6964.2021.03.013

Abstract

Abstract: The study aimed to analyze the proteomics of chicken seminal plasma and screen the differential proteins related to asthenospermia. The experimental roosters were selected from Ningdu yellow chicken hatched in the same batch, and semen quality was examined every 3 days,3 times in total. Four individuals with forward sperm ratio <20% and straight forward sperm ratio <10% were selected as asthenospermia group. Four individuals with forward sperm ratio >80% and straight forward sperm ratio >40% were selected as control group. Using liquid chromatography-mass spectrometry (LC-MS/MS) technology, the proteomics of chicken seminal plasma was studied and differential proteins related to asthenospermia were preliminary screened. The results showed that the sperm motility and density of roosters in asthenospermia group were extremely significantly lower than those in control group(P<0.01). A total of 78 differentially expressed proteins were screened out between the two groups. Compared with control group, 5 differential proteins were up-regulated and the others were down-regulated in the asthenospermia group. It is important to note that the expression of keratin 9 (KRT9) and selenoprotein P-2 (SEPP2) were only detected in asthenospermia group, and the expression of 4-hydroxy-2-oxoglutaracturonase (HOGA1), phenylalanine hydroxylase (PAH) and lecithin cholesterol acyltransferase (LCAT) were only detected in the control group. According to GO and KEGG analysis, the functions of differential proteins were mainly focused on metabolism, glycolysis, carbon metabolism, amino acid synthesis pathways, and so on. The differentially expressed proteins in seminal plasma of Ningdu yellow chickens in asthenospermia and control groups screened in this study could be used as candidate targets for screening and treatment of asthenospermia in chickens. The further functional analysis and verification will provide a basis for elucidating the pathogenesis of asthenospermia.

Key words: chicken, asthenospermia, liquid chromatography-mass spectrometry (LC-MS/MS) technology, differentially expressed proteins

CLC Number:

S831.3

WEI Yue, LI Yunlei, SUN Yanyan, WU Yanping, TANG Weiguo, CHEN Jilan, XIE Jinfang. Seminal Plasma Proteomics Analysis Associated with Asthenospermia in Rooster[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 693-702.

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Seminal Plasma Proteomics Analysis Associated with Asthenospermia in Rooster

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