鸭疫里默氏杆菌RA-LZ01株GE296_RS01450基因介导其对重金属离子产生耐受性的分析

doi:10.11843/j.issn.0366-6964.2021.010.017

Abstract

Abstract: This study was conducted to clarify the role of GE296_RS01450 gene of Riemerella anatipestifer strain RA-LZ01 in mediating its tolerance to heavy metal ions. We constructed GE296_RS01450 gene deletion mutant Δ1450 and complemented strain cΔ1450. The growth curves of these strains, minimum inhibitory concentration (MIC) of antibiotics against them and their tolerance to heavy metal ions were determined, and the transcription levels of GE296_RS01450 gene of these strains treated with specific heavy metal ions were detected. The results showed that the growth ability of the deletion mutant and the MIC values of 11 classes of antibiotics to deletion mutant did not change significantly by comparison with the parent strain. By compared with parent strain and complemented strain, the sensitivity of deletion mutant to Ni, Mn and Co increased significantly. The transcription levels of GE296_RS01450 gene of parent strain treated with Ni and Mn were significantly up-regulated. The above results indicate that the GE296_RS01450 gene does not involve in the growth of the parent strain and the strain's resistance to antibiotics. However, the gene mediates the parent strain's tolerance to Ni, Mn and Co. GE296_RS01450 gene belongs to the GE296_RS01445-GE296_RS01450-GE296_RS01455 RND efflux pump and encodes the outer membrane protein. This protein is named RopM.

Key words: Riemerella anatipestifer, GE296_RS01450 gene, growth, antibiotics, heavy metal ions

CLC Number:

S852.61

QIN Mingxing, GONG Xiaowei, CHEN Qiwei, WANG Yanping, QUAN Heng, ZHU Yujia, ZHENG Fuying, LIN Guozhen. Study on the GE296_RS01450 Gene-mediated Tolerance of Rimerella anatipestifer Strain RA-LZ01 to Heavy Metal Ions[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2864-2873.

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Study on the GE296_RS01450 Gene-mediated Tolerance of Rimerella anatipestifer Strain RA-LZ01 to Heavy Metal Ions

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