A:L1 ST128型鸭源多杀性巴氏杆菌的耐药性及毒力分析

doi:10.11843/j.issn.0366-6964.2021.010.016

Abstract

Abstract: The aim of this study was to characterize the drug resistance and virulence of pathogens from ducks suspected of cholera in a farm in Guizhou. The pathogenic bacteria were cultured and confirmed by PCR. Capsule genotyping and lipopolysaccharide genotyping, multilocus sequence typing (MLST), ERIC-PCR, animal regression test, drug susceptibility test, whole genome sequencing(WGS) and comparative analysis of local genomes were conducted on those isolated strains. Five Pasteurella multocida isolates (PmCW1-5) were identified as A:L1 ST128 and shared high homology. All of them were resistant to ampicillin, amoxicillin, levofloxacin, and lincomycin. PmCW1 also showed low level resistance to ciprofloxacin. WGS data showed PmCW1 which is highly virulent also harbored genes mediating β-lactams, quinolones, tetracyclines, macrolides and peptides resistance and encoding multi-drug efflux pumps and multi-drug resistance proteins. Meanwhile, PmCW1 carried 201 different virulence-mediating genes including those encoding lipooligosaccharide/lipopolysaccharide (LOS/LPS), capsule, adhesion factor, and etc. In addition, type IV fimbriae genes (ptfA, comE, hofB, hofC, vfr), iron uptake related protein genes (ccmABCEF, hgbBC, fur, hscB), outer membrane protein genes (ompP5) were also detected. PmCW1 has the characteristics of typical A:L1 P. multocida. P. multocida ST128 isolated from ducks in this study has been rarely reported. The drug resistance and virulence analysis on those isolates will provide theoretical basis for clinical medication and vaccine development of duck cholera.

Key words: Pasteurella multocida from ducks, isolation and identification, MLST, drug resistance and virulence, comparative analysis of local genomes

CLC Number:

S852.61

ZHANG Yanan, LI Yafei, CHEN Rujia, YU Bo, PENG Shan, LI Ting, PU Ling, XU Jinge. Resistance and Virulence Analysis of Type A: L1 ST128 Pasteurella multocida from Ducks[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2852-2863.

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Resistance and Virulence Analysis of Type A: L1 ST128 Pasteurella multocida from Ducks

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