水禽StAR基因克隆、表达及其对睾丸发育的影响

doi:10.11843/j.issn.0366-6964.2020.12.011

Abstract

Abstract: The purpose of this study was to obtain the structure and tissue expression of the StAR gene, and to initially explore its effect on the development of testis in waterfowl. The hypo-thalamus and testis of Shanma duck and Shitou goose were selected as materials to clone the StAR genes, and their expression patterns in different tissues of Shanma duck and Shitou goose were detected by real-time fluorescent quantitative PCR, and the differential expression of the genes in goose testis tissues in different periods were further compared. In this study, three transcripts of the duck StAR gene (dSTAR-A, dSTAR-B, and dSTAR-C) were obtained. The coding sequences of dSTAR-A and dSTAR-B were the same, encoding 283 amino acids, while dSTAR-C encoded 238 amino acids. Two transcripts of goose StAR gene (gSTAR-A and gSTAR-B) were obtained, both encoding 283 amino acids. The comparative analysis showed that the amino acid sequence of waterfowl StAR was highly homologous with other birds and highly conserved among species. The StAR gene was expressed in various tissues of ducks and geese, of which the highest expression level was in the testis, and it had higher expression levels in abdominal fat, breast muscle and leg muscle. Comparing the testis of male geese in different periods, it was found that the expression level of StAR gene was extremely significantly higher at age 3 years than that at age 1 and 52 days(P<0.01), and was extremely significantly higher in breeding period of adult male geese than that in restoration period (P<0.01). The results indicates that StAR gene may be a key gene for goose testis development and participate in the regulation of reproductive performance of adult male geese.

Key words: waterfowl, StAR, gene cloning, gene expression, reproduction

CLC Number:

OUYANG Hongjia, SUN Jingshuai, JIANG Danli, PAN Jianqiu, HUANG Yunmao. Cloning and Expression of Waterfowl StAR Gene and Its Effect on Testicular Development[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(12): 3013-3022.

References 23

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Cloning and Expression of Waterfowl StAR Gene and Its Effect on Testicular Development

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