高铜对大鼠肾细胞炎性因子和细胞增殖的影响

doi:10.11843/j.issn.0366-6964.2020.11.023

Abstract

Abstract: The purpose of this research was to investigate the effect of copper poisoning on the expression of inflammatory factors and cell proliferation in renal tissues of rat. In this study, 32 healthy rats aged 20 days were selected and randomly divided into 4 groups, 8 in each group. The rats were fed as follows: The copper concentration in the control group was 15 mg·kg^-1; high-copper group I: 30 mg·kg^-1; high-copper group Ⅱ: 60 mg·kg^-1; high-copper group Ⅲ: 120 mg·kg^-1. After continuously feeding for 6 months, the mRNA and their proteins expression of Ki-67, PCNA, IL-1β, IL-2, IL-6, IL-18, NF-κB, TNF-α in renal tissues were detected. The results demonstrated that the relative expressions of Ki-67，PCNA mRNA and proteins in renal tissues were significantly decreased with the increase of copper content in feed (P<0.05). Meanwhile, the mRNA expression levels of inflammatory factors including IL-1β, IL-2, IL-6, IL-18, NF-κB, TNF-α in renal tissues were significantly increased (P<0.05). Besides, the relative expression of IL-1β protein was up-regulated in a dose dependent manner, and the relative expression levels of IL-6 and IL-18 proteins increased first and then decreased. In summary, the finding suggested that high copper stimulation inhibited the cell proliferation of rat kidney tissue, promoted the expression of inflammatory factors, and caused inflammatory damage.

Key words: copper, renal, cell proliferation, inflammatory factors, rat

CLC Number:

S856.9

YU Qingqing, ZHONG Gaolong, WAN Fang, NING Zhijun, WU Shaofeng, JIANG Xuanxuan, TANG Zhaoxin, HE Ying, HU Lianmei. Effects of High Copper on Inflammatory Factors and Cell Proliferation in Rat Kidney Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2849-2857.

References 34

[1]	张倚剑,梁天柱,冯栋梁,等.微量元素铜在养殖业中的应用研究[J].广西农学报,2019,34(3):57-62.ZHANG Y J,LIANG T Z,FENG D L,et al.Research on trace element copper application in aquaculture[J].Journal of Guangxi Agriculture,2019,34(3):57-62.(in Chinese)
[2]	WANG Z H,LIU X Y,QIN H Y.Bioconcentration and translocation of heavy metals in the soil-plants system in Machangqing copper mine,Yunnan Province,China[J].J Geochem Explor,2019,200:159-166.
[3]	柏云江.铜锌砷等过量对饲料安全的影响及对策[J].现代畜牧兽医,2010(3):55-57.BAI Y J.Effect on feed safety of superfluous addition for Cu,Zn,As and countermeasure[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2010(3):55-57.(in Chinese)
[4]	杨光,崔恒敏,邓俊良,等.实验性雏鸡铜中毒的病理学研究[J].中国兽医科技,2005,35(10):801-806.YANG G,CUI H M,DENG J L,et al.Pathological study of experimental copper toxicity in chickens[J].Chinese Journal of Veterinary Science and Technology,2005,35(10):801-806.(in Chinese)
[5]	WAN F,ZHONG G L,NING Z J,et al.Long-term exposure to copper induces autophagy and apoptosis through oxidative stress in rat kidneys[J].Ecotoxicol Environ Saf,2020,190:110158.
[6]	ZHAO H J,WANG Y,FEI D X,et al.Destruction of redox and mitochondrial dynamics co-contributes to programmed cell death in chicken kidney under arsenite or/and copper (II) exposure[J].Ecotoxicol Environ Saf,2019,179:167-174.
[7]	于燕波,张淑静,臧鹏,等.低剂量有机磷农药和重金属联合暴露对大鼠白细胞介素、肿瘤坏死因子及免疫球蛋白的影响[J].卫生研究,2014,43(2):317-319.YU Y B,ZHANG S J,ZANG P,et al.Effects of low doses of organophosphorus pesticides combined with heavy metals on interleukin,tumor necrosis factor and immunoglobulin in rats[J].Journal of Hygiene Research,2014,43(2):317-319.(in Chinese)
[8]	JIAO T,CHU X H,GAO Z Q,et al.New insight into the molecular basis of Fe (III) stress responses of Procambarus clarkii by transcriptome analysis[J].Ecotoxicol Environ Saf,2019,182:109388.
[9]	任洁,程春来,芦兰,等.肺癌血清标志物PCNA及Bax的表达及与预后的关系[J].重庆医学,2017,46(10):1399-1401.REN J,CHENG C L,LU L,et al.Expression of lung cancer serum markers PCNA and Bax and their relationship with prognosis[J].Chongqing Medical Journal,2017,46(10):1399-1401.(in Chinese)
[10]	CHI J R,YU Z H,LIU B W,et al.SNHG5 promotes breast cancer proliferation by sponging the miR-154-5p/PCNA axis[J].Mol Ther Nucleic Acids,2019,17:138-149.
[11]	郑皓,景嘉楠,董慧,等.细胞周期蛋白依赖性激酶16(CDK16)与细胞增殖指标Ki67在食管鳞癌中的表达及意义[J].西安交通大学学报:医学版,2019,40(3):425-431.ZHENG H,JING J N,DONG H,et al.Expression and significance of cyclin-dependent kinase 16 and cell proliferation index Ki67 in esophageal squamous cell carcinoma[J].Journal of Xi'an Jiaotong University:Medical Sciences,2019,40(3):425-431.(in Chinese)
[12]	LI L T,JIANG G,CHEN Q,et al.Ki67 is a promising molecular target in the diagnosis of cancer (review)[J].Mol Med Rep,2015,11(3):1566-1572.
[13]	刘云才.低剂量铜暴露对大鼠肝功能和炎性因子表达的影响及乳酸菌干预作用[D].青岛:青岛大学,2017.LIU Y C.Effects of low dose copper exposure on liver function and expression of inflammatory cytokines in rats and intervention of Lactic acid bacteria[D].Qingdao:Qingdao University,2017.(in Chinses)
[14]	CHEN J W,STEFANOVSKI D,HAUGHAN J,et al.Inflammatory mediators are potential biomarkers for extracorporeal shockwave therapy in horses[J].Equine Vet J,2020,52(3):449-454.
[15]	LIU X Y,CHEN G N,DU G M,et al.Berbamine ameliorates ethanol-induced liver injury by inhibition of hepatic inflammation in mice[J].Chin J Nat Med,2020,18(3):186-195.
[16]	HILL G M,SHANNON M C.Copper and zinc nutritional issues for agricultural animal production[J].Biol Trace Elem Res,2019,188(1):148-159.
[17]	刘伟.猪五种常见潜在有毒物质中毒症的诊疗[J].现代畜牧科技,2019(2):103-104.LIU W.Diagnosis and treatment of five common potential toxic substance poisoning diseases in pigs[J].Modern Animal Husbandry Science & Technology,2019(2):103-104.(in Chinese)
[18]	郭剑英,余文兰,曹华斌,等.铜对大鼠肝细胞活性、自由基代谢及细胞膜电位的影响[J].中国兽医学报,2016,36(11):1939-1943,1948.GUO J Y,YU W L,CAO H B,et al.The effects of copper on rat hepatocytes proliferation activity and its free radical metabolism and the membrane potential[J].Chinese Journal of Veterinary Science,2016,36(11):1939-1943,1948.(in Chinese)
[19]	邱文才,蔡跃佳,李伟豪,等.铜中毒对小鼠心肺及肝功能的影响[J].动物医学进展,2019,40(11):55-58.QIU W C,CAI Y J,LI W H,et al.Effects of copper poisoning on cardiac,pulmonary and hepatic functions in mice[J].Progress in Veterinary Medicine,2019,40(11):55-58.(in Chinese)
[20]	GIERGIEL M,DURKALEC M M,NAWROCKA A,et al.Ingestion of bedding material as a cause of acute copper sulfate poisoning in turkey poults[J].Poult Sci,2019,98(2):707-711.
[21]	LIN X M,WEI G,HUANG Z J,et al.Mitochondrial proteomic alterations caused by long-term low-dose copper exposure in mouse cortex[J].Toxicol Lett,2016,263:16-25.
[22]	付世新,罗春海,刘海瑞.铜对猪传代肾细胞(PK15)增殖的影响[J].黑龙江八一农垦大学学报,2008,20(2):47-50.FU S X,LUO C M,LIU H R.Effect of copper on proliferation of PK15[J].Journal of Heilongjiang August First Land Reclamation University,2008,20(2):47-50.(in Chinese)
[23]	张静,曲小姝,刘树萍,等.过渡金属铜修饰的仲钨酸盐抑制人卵巢癌细胞增殖的研究[J].东北师大学报:自然科学版,2017,49(3):92-96.ZHANG J,QU X S,LIU S P,et al.Inhibitory effect of a paradodecatungstate-B compound decorated by transition metal copper on the growth of human ovarian carcinoma SKOV-3 cells[J].Journal of Northeast Normal University:Natural Science Edition,2017,49(3):92-96.(in Chinese)
[24]	PEREIRA T C P,CAMPOS M M,BOGO M R.Copper toxicology,oxidative stress and inflammation using zebrafish as experimental model[J].J Appl Toxicol,2016,36(7):876-885.
[25]	KENNETH N,HUCKS G,KOCAB A,et al.Copper is a potent inhibitor of both the canonical and non-canonical NFκB pathways[J].Cell Cycle,2014,13(6):1006-1014.
[26]	李敏.增殖细胞核抗原和肿瘤增殖标记分子在胃癌中的表达及临床意义[D].泸州:西南医科大学,2019.LI M.Expression and clinical significance of proliferating cell nuclear antigen and tumor proliferation marker molecule in gastric cancer[D].Luzhou:Southwest Medical University,2019.(in Chinese)
[27]	QIU X M,WANG H,WANG Z,et al.Expression of PCNA,Ki-67 and COX-2 in breast cancer based on DCE-MRI image information[J].J Infect Public Health,2019,doi:10.1016/j.jiph.2019.06.024.
[28]	文丹,廖媛,李健春,等.异甘草素通过Smad3/Arid2-IR/NF-κB轴改善顺铂诱导的急性肾损伤小鼠炎症反应[J].中国比较医学杂志,2020,30(6):25-30,46.WEN D,LIAO Y,LI J C,et al.Effect of isoliquiritigenin on cisplatin-induced acute kidney injury in mice with inflammation through the Smad3/Arid2-IR/NF-κB axis[J].Chinese Journal of Comparative Medicine,2020,30(6):25-30,46.(in Chinese)
[29]	TURNLUND J R,KEYES W R,KIM S K,et al.Long-term high copper intake:effects on copper absorption,retention,and homeostasis in men[J].Am J Clin Nutr,2005,81(4):822-828.
[30]	FUNSTEN J R,BRIZUELA K O M,SWATZEL H E,et al.PKC signaling contributes to chromatin decondensation and is required for competence to respond to IL-2 during T cell activation[J].Cell Immunol,2020,347:104027.
[31]	聂兆超,石星,毛海婷.IL-18诱导的单核细胞THP-1对肺癌细胞增殖、迁移和侵袭的影响[J].南京医科大学学报:自然科学版,2019,39(10):1430-1436.NIE Z C,SHI X,MAO H T.Effects of IL-18-induced mononuclear cell THP-1 on proliferation,migration and invasion of lung cancer cells[J].Journal of Nanjing Medical University:Natural Sciences,2019,39(10):1430-1436.(in Chinese)
[32]	OKAMURA H,TSUTSUI H,KASHIWAMURA S I,et al.Interleukin-18:a novel cytokine that augments both innate and acquired immunity[J].Adv Immunol,1998,70:281-312.
[33]	赵洪礼,吴战军.细胞因子与重症急性胰腺炎关系研究进展[J].临床消化病杂志,2010,22(1):61-63.ZHAO H L,WU Z J.Research progress on the relationship between cytokines and severe acute pancreatitis[J].Chinese Journal of Clinical Gastroenterology,2010,22(1):61-63.(in Chinese)
[34]	LUZARDO-OCAMPO I,CAMPOS-VEGA R,DE MEJIA E G,et al.Consumption of a baked corn and bean snack reduced chronic colitis inflammation in CD-1 mice via downregulation of IL-1 receptor,TLR,and TNF-α associated pathways[J].Food Res Int,2020,132:109097.

Effects of High Copper on Inflammatory Factors and Cell Proliferation in Rat Kidney Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 34

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	MIAO Shu, AN Jishan, WANG Zuo, XIAO Dingfu, LAN Xinyi, LIU Lei, SHEN Weijun, WAN Fachun. Leucine Promotes the Proliferation of Bovine Myoblasts through PI3K-AKT Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 142-152.
[2]	DUAN Xiangru, KANG Jia, YANG Ruochen, SHAN Xinyu, LI Taichun, ZHAO Wen, ZHANG Yingjie, LIU Yueqin. Effect of L-cysteine on Proliferation, Apoptosis and the Secretion of Steroid Hormone in Ovine Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 179-191.
[3]	SONG Wenyan, ZHANG Hanwen, WU Aodi, ZHANG Liyan, LIU Zhao, YE Tongtong, CHEN Chuangfu, SHENG Jinliang. Screening of Nanobodies against Porcine Reproductive and Respiratory Syndrome Virus GP5 Protein and Exploration of Their Inhibitory Effect on Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 258-270.
[4]	TIAN Qihui, ZHANG Liang, LONG Yali. Study on the Effect of Astragalus on Proliferation of Bone Marrow Mesenchymal Stem Cells in Anoxic Microenvironment based on PI3K-AKT Signal Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 346-354.
[5]	WANG Zhiyuan, LIU Boqi, XU Zhiying, XU Sijia, XING Jiabao, ZHANG Guihong, WANG Heng, SUN Yankuo. Analysis of ORF5 Gene Variation of Porcine Reproductive and Respiratory Syndrome Virus in Local Regions of China from 2021 to 2022 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3812-3823.
[6]	FAN Yandi, YANG Danjiao, YE Zhongming, ZHANG Min, LAN Lan, WANG Jinghao, ZHOU Han, KANG Runmin, YU Jifeng, ZHANG Zhidong, LI Yanmin, ZHOU Long. Metagenomic Analysis of Viruses in Clinical Samples of Respiratory Diseases in Tibetan Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3836-3847.
[7]	ZHANG Yafeng, ZHU Bin, MA Chang, ZHANG Yuanshu. The Research on the Effects of ACE2 Activated by Diminazene Aceturate on Mitochondria in the Liver of Rats with Non-alcoholic Fatty Liver Disease [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3895-3904.
[8]	CHEN Hong, RUAN Hongri, MA Tianwen, LI Yanan, MIAO Xue, YANG Wenyue, GAO Li, WEI Chengwei. The Mechanism of Puerarin Improving Cartilage Degeneration in PTOA Rats by Interfering with Oxidative Stress and Nrf2/HO-1 Pathway of Cartilage [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3951-3963.
[9]	WANG Ziyue, ZHANG Zihui, WU Wenxue, PENG Chen. Monkeypox: Diagnosis, Prevention and Treatments [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3195-3205.
[10]	ZHANG Wanfeng, ZHAO Tianzhi, LI Jiao, YOU Ziwei, YANG Yang, CAI Chunbo, GAO Pengfei, CAO Guoqing, GUO Xiaohong, LI Bugao. Study on NR2F2 Gene Regulating Proliferation and Apoptosis of Porcine PK15 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3242-3251.
[11]	HE Mingyang, MA Yujing, WANG Yong, YANG Ruochen, LIU Yueqin, ZHANG Yingjie, DUAN Chunhui. Effects of Melatonin on Proliferation, Apoptosis of Ovarian Granulosa Cells, and Its Secretion of Steroid Hormones of Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3313-3324.
[12]	SONG Meijun, HAO Kexing, HAI Siyu, CHEN Yan, WANG Jing, HU Guangdong. Effects of SRIF-14 on Proliferation and Apoptosis of Endometrial Epithelial Cells in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3325-3334.
[13]	WANG Wanjie, CHEN Nanzhu, ZOU Huiying, ZHOU Xinyi, HAO Haisheng, PANG Yunwei, ZHU Huabin, ZHAO Xueming, YU Dawei, DU Weihua. Effects of Histone Methyltransferase ASH1L Overexpression on Proliferation and Apoptosis of Bovine Cumulus Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3358-3368.
[14]	ZHANG Ying, JIN Hua, HAN Yang, SUI Dan, XIAO Xin, HAO Xiujing, LI Min. Analysis of Microbial Diversity in Nasal Cavity of Sheep with Respiratory Disease and Their Environment [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3455-3465.
[15]	SHEN Tong, WANG Mengjie, WU Hua, LI Jinming, WANG Shuo, WU Xiaoqing, CHEN Xinlei, XING Qianwen, LIU Bo. Effect of Lycium ruthenicum Murray Anthocyanin on Hypoxia-induced Apoptosis in H9c2 Rat Cardiomyocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3490-3499.