脂肪酸结合蛋白4对BCG诱导巨噬细胞自噬的调控作用

doi:10.11843/j.issn.0366-6964.2020.09.024

Abstract

Abstract: To investigate the role of FABP4 on regulating fatty acid metabolism and autophagy in macrophage infected with BCG, small interfering RNA for FABP4 were transfected into RAW264.7 cells alone or combined with BCG infection. The immunoblot and immunofluorescence were used to detect the expression of FABP4, the accumulation of lipid droplets, the expression of fatty acid β-oxidation and autophagy related factors. The results showed that the infection of BCG increased the expression of FABP4 and accompanied with the accumulation of lipid droplets in RAW264.7. Moreover, the knockdown of FABP4 decreased lipid droplets but promoted the expression of carnitine palmitoyltransferase 1A (CPT1A) (P <0.05) and the production of ATP (P <0.05). Meanwhile, the knockdown of FABP4 suppressed the expression of AMPK, p-ULK1, ATG5, ATG7, ATG12 and LC3B which are autophagy related factors (P<0.001). Our results indicated that the knockdown of FABP4 promoted fatty acid oxidation, decreased fatty acid level and suppressed autophagy via AMPK signal pathway in BCG infected RAW264.7 cells.

Key words: FABP4, BCG, macrophage RAW264.7, fatty acid metabolism, autophagy

CLC Number:

S855.2

YU Jialin, XU Yanan, HAN Lu, MA Qinmei, WU Xiaoling, DENG Guangcun. Role of Fatty Acid Binding Protein 4 in Regulating Macrophage Autophagy Induced by BCG Infection[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2265-2274.

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Role of Fatty Acid Binding Protein 4 in Regulating Macrophage Autophagy Induced by BCG Infection

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