山羊INSR基因可变剪接体在背最长肌中的表达模式

doi:10.11843/j.issn.0366-6964.2020.08.006

Abstract

Abstract: The study was conducted to perform alternative splicing analysis on goat insulin receptor (INSR) gene, and to analyze its expression profiles in goat Longissimus dorsi muscle. The Longissimus dorsi muscle of Jianzhou Daer goat were collected from fetuses at 45, 60, and 105 days of gestation and lambs at the 3rd day after birth. Based on Sanger sequencing and high throughput sequencing data (accession number: SRR3567144), the alternative splicing patterns of the INSR gene and their expression difference in Longissimus dorsi muscle were studied. In addition, the characteristics of different alternative splicing variants of goat INSR gene were analyzed using online softwares. The results showed that 4 different INSR transcripts were identified and designated as INSR1, INSR2,INSR3 and INSR4, with CDS lengths of 4 110, 4 146, 4 113 and 4 149 bp, respectively, and the correspondingly amino acid residues were 1 369, 1 381, 1 370 and 1 382. The Exon 11 (36 bp) skipping and partial deletion of Exon 15 (3 bp) led to the main difference among 4 transcripts of INSR in goat. The two alternative splicing patterns were completely identical among INSR gene transcripts of bovine, pig, goat, human, sheep and mouse. These alternative splicing changed the serine phosphorylation sites and the second FN3 domain of goat INSR, and resulted in 3D structure change of the encoded protein in two regions. Meanwhile, there was no significant difference in the expression level of 4 splicing variants during 4 Longissimus dorsi muscle developmental periods of goats (P>0.05), and there was no significant difference among the expression level of 4 splicing variants within the same developmental period (P>0.05). In conclusion, the sequence and splicing model of INSR gene's splicing variants are highly conserved among mammals, and the results have provided the theoretical reference for further revealing the role of INSR gene in regulating animal muscle development.

Key words: goat, INSR, splicing transcripts, expression patterns

CLC Number:

S827.2

ZHANG Xiao, LI Li, ZHANG Hongping. Identification of Splicing Transcripts of Insulin Receptor Gene and Their Expression Profiles in Goat Longissimus Dorsi Muscles[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8): 1823-1832.

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Identification of Splicing Transcripts of Insulin Receptor Gene and Their Expression Profiles in Goat Longissimus Dorsi Muscles

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