一株单核细胞增生李斯特菌分离株的分子分型鉴定及其生物学特性

doi:10.11843/j.issn.0366-6964.2020.05.021

Abstract

Abstract: An isolate of Listeria monocytogenes CMG47 was obtained from a goat farm in Shanghai. To identify the genotyping and biological characteristics of this isolate, multiplex PCR was performed in lineage identification and serotyping, multi-locus sequence typing (MLST) was used for genotyping. Major virulence factors were examined by PCR, hemolysis and phospholipase activity were analyzed by in vitro observation and real-time fluorescent quantitative PCR. The virulence was determined by intraperitoneal injection in ICR mice and intravenous injection in zebrafish. Study results revealed that the isolate belonged to lineage I, 1/2b for its serotyping, ST619 for its genotyping, and carried major virulence factors such as prfA, inlA, inlB, plcA, plcB, mpl, actA and hly. It showed weak hemolysis but did not exhibit apparent phospholipase activity. The isolate was of high pathogenicity comparable to the reference virulent strain EGDe in mice and zebrafish models (P>0.05). The isolate belongs to the dominant serotyping and genotyping for Listeria infection, which carries major virulence factors and is highly pathogenic. This work provides the molecular basis for further analysis of the prevalence and distribution characteristics of sporadic listeriosis and is very important for the establishment of Listeria monitoring system and risk assessment as well.

Key words: Listeria monocytogenes, multi-locus sequence typing (MLST), virulence

CLC Number:

XIN Yongping, SHAN Ying, XIA Ye. Genotyping Analysis and Biological Characteristics of a Clinical Listeria monocytogenes Isolate[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1101-1109.

References 30

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Genotyping Analysis and Biological Characteristics of a Clinical Listeria monocytogenes Isolate

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