KLF11抑制山羊肌内前体脂肪细胞分化

doi:10.11843/j.issn.0366-6964.2020.05.009

Abstract

Abstract: This study was performed to obtain the sequence of Krüppel-like factor 11 (KLF1) gene, clarify its expression pattern in tissues and cells of goats, and further elucidate its effects on the differentiation of goat intramuscular preadipocytes with its possible action pathway. The expression of KLF11 in goat different tissues and cells at different adipogenic differentiation stages was detected by real-time fluorescence quantitative PCR (qPCR). From both morphology and molecular biology, Oil red O staining and qPCR were used to reveal the effect of interfering KLF11 on the differentiation of intramuscular adipocytes of goats with its possible action pathway. The results showed that KLF11 gene was 1 752 bp in length, CDS region was 1 518 bp in length, which encoded 505 amino acids. KLF11 gene was widely expressed in the various tissues of goats. The expression level of KLF11 was higher in liver, longissimus dorsi and abdominal fat. Compared with the preadipocytes, the expression of KLF11 gene extremely significantly increased at 48 h of differentiation(P<0.01).After obtaining the effective interference sequence of KLF11-siRNA1, the expression of KLF11 was significantly decreased by KLF11-siRNA1 to 63.58%, obviously promoted accumulation of lipid droplets were odserved in the intramuscular adipocytes of goats. After interfering KLF11, the mRNA levels of PPARγ and C/EBPβ, the adipocyte differentiation marker genes were extremely significantly up-regulated (P<0.01), while the expression of Pref-1 was extremely significantly down-regulated (P<0.01). Meanwhile, the expression levels of some members of KLFs family (KLF1,KLF2,KLF4,KLF5,KLF8,KLF10,KLF14,KLF16) significantly declined and others increased after interfering KLF11 gene. KLF11 gene could inhibit the differentiation of goat intramuscular preadipocytes through regulating the expression of PPARγ, C/EBPβ and Pref-1, and this effect might be achieved by synergizing with KLF2, KLF4, KLF10 and antagonizing with KLF7, KLF15.

Key words: goat, KLF11 gene, intramuscular preadipocytes, RNAi, cell differentiation

CLC Number:

S827.2

WANG Jianglin, WANG Yong, CHI Yongdong, ZHU Jiangjiang, LIN Yaqiu. KLF11 Inhibits the Differentiation of Goat Intramuscular Preadipocyte[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 976-986.

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KLF11 Inhibits the Differentiation of Goat Intramuscular Preadipocyte

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