性激素受体在子午岭黑山羊隐睾及正常睾丸中的分布比较

doi:10.11843/j.issn.0366-6964.2020.029

Abstract

Abstract: To study the relationship between the distribution of sex hormone receptors (AR and ER) in normal and cryptorchid testis and cryptorchidism of the Ziwuling black goat, the histochemical characteristics of normal testis and cryptorchidism were compared by immunohistochemistry, immunofluorescence and morphometric statistical software. The results of special staining showed that compared with the normal group, the interstitial tissue of cryptorchidism group was loose, the lumen area was significantly reduced, the content of glycogen was significantly decreased, and the contents of collagen and reticular fibers were increased. The results of immunohistochemistry and immunofluorescence showed that:1) AR was strongly expressed in Leydig cells of the normal testis and moderately positive in all levels of spermatogenic cells, while the expression of Leydig cells and Spermatogenic cells in cryptorchidism group was significantly decreased and occasionally expressed in Spermatogonia. 2) ER was strongly expressed in the normal testicular Leydig cells, moderately positive in Peritubular myoid cells, occasionally expressed in Sertoli cells and not expressed in Spermatogenic cells. 3) ER was moderately positive in cryptorchidism Leydig cells, primary Spermatocytes, Spermatogonia and Sertoli cells. 4) The average optical density of AR in cryptorchidism group was significantly lower than that in the normal group, while the average optical density of ER in cryptorchidism group was significantly higher than that in the normal group. The ratio of AR to ER expression in cryptorchidism group was close to 1:1. The distribution of collagen and reticular fibers in cryptorchidism of Ziwuling black goat is more than that of the normal testis, and the main component of seminiferous tubule basement membrane is the neutral glycoprotein. The decrease of the content of acid glycoprotein may affect the normal formation of spermatozoa. The expression of ER and AR is abnormal during cryptorchidism, especially the expression of spermatogonia and Sertoli cells is opposite to that of AR, which can provide some reference for the related research of cryptorchidism in mammals.

Key words: Ziwuling black goat, androgen receptor, estrogen receptor, testis, cryptorchidism

CLC Number:

S858.277.29

WANG Qianmei, YUAN Ligang, LI Chengye, YANG Hongzao, CHEN Shaoyu. Distribution of Sex Hormone Receptors in Cryptorchidism and Normal Testes of Ziwuling Black Goat[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1455-1465.

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Distribution of Sex Hormone Receptors in Cryptorchidism and Normal Testes of Ziwuling Black Goat

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