性成熟期辽宁绒山羊与子午岭黑山羊睾丸发育比较

doi:10.11843/j.issn.0366-6964.2021.012.013

Abstract

Abstract: The study aimed to investigate the differences in testicular development between Liao-ning cashmere goat and Ziwuling black goat at sexual maturity and to compare the reproductive performance between the two breeds. In this study, testicular tissues were collected from five healthy Liaoning cashmere goats and five healthy Ziwuling black goats at sexual maturity stage. The testicular tissue development and morphological differences between the two goat breeds were compared using anatomy and Hematoxylin-eosin (HE) stained, respectively. The concentration of androgen were detected using ELISA assay. The expression of DEAD box polypeptide 4 (DDX4) and Deleted in azoospermia-like gene (DAZL) in testis of the two breeds were detected using Real-time quantitative PCR(RT-qPCR) and Western blot technique. The study revealed that the total weight and length circumference of testicular in Liaoning cashmere goats were significantly higher than those in Ziwuling black goats (P<0.01), while the difference testicular breadth circumference, total testicular weight/pre-slaughter live weight and total testicular weight/carcass weight between the two goat breeds were not significant (P>0.05). The spermatogenic epithelial thickness of Liaoning cashmere goats was significantly higher than that of Ziwuling black goats (P<0.05), there were no significant differences in the area and diameter of the seminal tubules, number of seminal tubules per unit area between the two goat breeds (P>0.05). The difference of androgen secretion in testis between the two goat breeds was not significant (P>0.05). The expression levels of DDX4 mRNA and DDX4 protein in the testis of Liaoning cashmere goats were significantly higher than those of Ziwuling black goats (P<0.01 or P<0.05). The expression levels of DAZL mRNA in the testis of Liaoning cashmere goats was significantly higher than that of Ziwuling black goats (P<0.01), while the expression of DAZL protein between two goat breeds was not significant (P>0.05). In short, the degree of gonad development in Liaoning cashmere goats was identical with Ziwuling black goats, while the spermatogenic epithelium was thicker than Ziwuling black goats, there were relevant difference in the expression levels of reproductive marker genes between the two goat breeds, which might affect the spermatogenic ability between the two breeds.

Key words: Liaoning cashmere goat, Ziwuling black goat, testes, DDX4, DAZL

CLC Number:

S827.3

LIANG Weiwei, LI Mingna, LUO Yuzhu, WANG Jiqing, KE Na, SHEN Jiyuan, HAO Zhiyun, JIN Xiayang, LU Yujie, HUANG Zhaochun. Comparation of the Testes Development between Liaoning Cashmere Goat and Ziwuling Black Goat at Sexual Maturity[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3461-3470.

References 41

[1]	刘欣.辽宁绒山羊的繁殖特点及提高繁殖成活率的有效方法[J].畜禽业, 2020, 31(9):38, 40.LIU X.The reproductive characteristics of Liaoning cashmere goat and effective methods to increase the survival rate of reproduction[J].Livestock and Poultry Industry, 2020, 31(9):38, 40. (in Chinese)
[2]	杨术环, 邹丽娜.加快推进辽宁绒山羊产业发展的探索[J].现代畜牧兽医, 2020(7):52-54.YANG S H, ZOU L N.Explorations on accelerating development of Liaoning cashmere goat industry[J].Modern Journal of Animal Husbandry and Veterinary Medicine, 2020(7):52-54.(in Chinese)
[3]	JIN M, ZHANG J Y, CHU M X, et al.Cashmere growth control in Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 and decorin genes[J].Asian-Australas J Anim Sci, 2018, 31(5):650-657.
[4]	杨德智.陇东绒山羊改良历史、现状及发展方略探讨[J].畜牧兽医杂志, 2017, 36(6):87-90.YANG D Z.Discussion on the history, present situation and development strategy of Longdong cashmere goat[J].Journal of Animal Science and Veterinary Medicine, 2017, 36(6):87-90.(in Chinese)
[5]	马月辉, 蒋英.辽宁绒山羊杂交改良子午岭黑山羊的效果及遗传分析[J].畜牧兽医学报, 1992, 23(1):39-43.MA Y H, JIANG Y.The effects of improvement and genetic analyses on Ziwuling goat crossed with Liaoning cashmere goat[J].Acta Veterinaria et Zootechnica Sinica, 1992, 23(1):39-43.(in Chinese)
[6]	王继卿, 沈继源, 刘秀, 等.子午岭黑山羊与辽宁绒山羊产肉性能、肉品质、肌肉营养成分和脂肪酸含量比较[J].草业学报, 2021, 30(2):166-177.WANG J Q, SHEN J Y, LIU X, et al.Comparative analysis of meat production traits, meat quality, and muscle nutrient and fatty acid contents between Ziwuling black goats and Liaoning cashmere goats[J].Acta Prataculturae Sinica, 2021, 30(2):166-177.(in Chinese)
[7]	黄健红, 黄乘光, 林飞鸿.少精子症患者性激素水平与生殖细胞凋亡的关系[J].广东医学, 2008, 29(5):771-772.HUANG J H, HUANG C G, LIN F H.The relationship between sex hormone levels and germ cell apoptosis in patients with oligospermia[J].Guangdong Medical Journal, 2008, 29(5):771-772.(in Chinese)
[8]	吴剑锋.男性基础性激素水平与精子质量参数的相关性探讨[J].现代检验医学杂志, 2020, 35(3):69-71, 77.WU J F.Correlation between male basic sex hormones and sperm quality parameters[J].Journal of Modern Laboratory Medicine, 2020, 35(3):69-71, 77.(in Chinese)
[9]	杨麦贵, 杨阳, 黄萍, 等.精液中性激素水平与生殖细胞凋亡的关系[J].中华男科学杂志, 2006, 12(5):432-434.YANG M G, YANG Y, HUANG P, et al.Sexual hormone levels in semen and germ cell apoptosis[J].National Journal of Andrology, 2006, 12(5):432-434.(in Chinese)
[10]	白曼.小尾寒羊公羊繁殖性能研究及其睾丸发育miRNA-mRNA表达谱联合分析[D].长春:吉林农业大学, 2018.BAI M.Investigation of ram reproductive performance of Small Tail Han sheep and integrative analysis of miRNA-mRNA expression profiles in their testis development[D].Changchun:Jilin Agricultural University, 2018.(in Chinese)
[11]	薛茂云, 高玉时, 唐修君.文昌鸡血清雄激素水平对精液品质的影响[J].中国畜牧兽医, 2009, 36(9):184-185.XUE M Y, GAO Y S, TANG X J.The impact of the level of androgen on the quality of sperm in mature roosters[J].China Animal Husbandry & Veterinary Medicine, 2009, 36(9):184-185.(in Chinese)
[12]	SCHUPBACH T, WIESCHAUS E.Germline autonomy of maternal-effect mutations altering the embryonic body pattern of Drosophila[J].Dev Biol, 1986, 113(2):443-448.
[13]	ANDERSON R A, FULTON N, COWAN G, et al.Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis[J].BMC Dev Biol, 2007, 7:136.
[14]	周阳, 骆骅, 李伯江, 等.牦牛和犏牛睾丸组织DDX4基因mRNA表达水平与启动子区甲基化[J].中国农业科学, 2013, 46(3):630-638.ZHOU Y, LUO H, LI B J, et al.mRNA expression level and promoter methylation of DDX4 gene in testes of yak and cattle-yak[J].Scientia Agricultura Sinica, 2013, 46(3):630-638.(in Chinese)
[15]	TANAKA S S, TOYOOKA T, AKASU R, et al.The mouse homolog of Drosophila Vasa is required for the development of male germ cells[J].Genes Dev, 2000, 14(7):841-853.
[16]	ELLIOTT D J, BOURGEOIS C F, KLINK A, et al.A mammalian germ cell-specific RNA-binding protein interacts with ubiquitously expressed proteins involved in splice site selection[J].Proc Natl Acad Sci U S A, 2000, 97(11):5717-5722.
[17]	VANGOMPEL M J W, XU E Y.The roles of the DAZ family in spermatogenesis:More than just translation?[J].Spermatogenesis, 2011, 1(1):36-46.
[18]	付永.Dazl基因mRNA表达水平与犏牛雄性不育关系的研究[J].青海畜牧兽医杂志, 2012, 42(6):9-11.FU Y.The Study on the relationship between the expression level of Dazl gene mRNA and the infertility of cattle-yak[J].Chinese Qinghai Journal of Animal and Veterinary Sciences, 2012, 42(6):9-11.(in Chinese)
[19]	YUAN Z H, LUO J, WANG L, et al.Expression of DAZL gene in selected tissues and association of its polymorphisms with testicular size in Hu sheep[J].Animals, 2020, 10(4):740.
[20]	李讨讨, 马友记, 赵兴绪, 等.Dazl基因在绵羊不同发育期睾丸组织中的表达与细胞定位[J].农业生物技术学报, 2018, 26(3):421-428.LI T T, MA Y J, ZHAO X X, et al.Expression and cellular localization of Dazl gene in testis of sheep (Ovis aries) at different developmental stages[J].Journal of Agricultural Biotechnology, 2018, 26(3):421-428.(in Chinese)
[21]	赵有璋.羊生产学[M].3版.北京:中国农业出版社, 2010.ZHAO Y Z.Production science of sheep and goat[M].3rd ed.Beijing:China Agriculture Press, 2010.(in Chinese)
[22]	KHAN H, RIND M M, MEHMOOD M U, et al.Biometric assessment of the testis in pakistani adult male goats (Capra hircus)[J].Pak J Zool, 2014, 46(3):880-884.
[23]	国家畜禽遗传资源委员会.中国畜禽遗传资源志-羊志[M].北京:中国农业出版社, 2011.China National Commission of Animal Genetic Resources.Animal genetic resources in China-sheep and goats[M].Beijing:China Agriculture Press, 2011.(in Chinese)
[24]	周文静, 袁泽湖, 李熙成, 等.3种不同类型藏羊的屠宰性能和肉品质的比较分析[J].草业科学, 2019, 36(7):1869-1878.ZHOU W J, YUAN Z H, LI X C, et al.Comparison of carcass and meat quality among three different types of Tibetan sheep[J].Pratacultural Science, 2019, 36(7):1869-1878.(in Chinese)
[25]	李长存, 韩存魁, 杜燕红.辽宁绒山羊与当地山羊杂交改良效果调查[J].青海畜牧兽医杂志, 2008, 38(1):31-32.LI C C, HAN C K, DU Y H, et al.Investigation on the effect of hybrid improvement between Liaoning cashmere goats and local goats[J].Chinese Qinghai Journal of Animal and Veterinary Sciences, 2008, 38(1):31-32.(in Chinese)
[26]	杨冲, 李进, 徐振国, 等.辽宁绒山羊杂交改良本地山羊肉用性能的测定[J].中国畜牧杂志, 2001, 37(4):38.YANG C, LI J, XU Z G, et al.Determination of meat performance of Liaoning cashmere goats crossed and improved local goats[J].Chinese Journal of Animal Science, 2001, 37(4):38.(in Chinese)
[27]	赵云翔, 高广雄, 方程, 等.不同品种公猪睾丸、精液品质发育规律及相关性研究[J].中国农业科技导报, 2020, 22(7):61-68.ZHAO Y X, GAO G X, FANG C, et al.Developmental Rule and correlation of testicular and semen quality in different breeds of boars[J].Journal of Agricultural Science and Technology, 2020, 22(7):61-68.(in Chinese)
[28]	MACLEOD G, VARMUZA S.The application of proteomic approaches to the study of mammalian spermatogenesis and sperm function[J].FEBS J, 2013, 280(22):5635-5651.
[29]	ZHOU R, WU J R Z, LIU B, et al.The roles and mechanisms of Leydig cells and myoid cells in regulating spermatogenesis[J].Cell Mol Life Sci, 2019, 76(14):2681-2695.
[30]	TREMBLAY J J.Molecular regulation of steroidogenesis in endocrine Leydig cells[J].Steroids, 2015, 103:3-10.
[31]	彭占威.关于用保持适度低量雄性激素来延长男性寿命的研究[J].医药前沿, 2013(1):115.PENG Z W.Research on prolonging male lifespan by keeping moderately low levels of male hormones[J].Journal of Frontiers of Medicine, 2013(1):115.(in Chinese)
[32]	REBOURCET D, O'SHAUGHNESSY P J, MONTEIRO A, et al.Sertoli cells maintain Leydig cell number and peritubular myoid cell activity in the adult mouse testis[J].PLoS One, 2014, 9(8):e105687.
[33]	LIU Z S, LI Q F, PAN Z X, et al.Comparative analysis on mRNA expression level and methylation status of DAZL gene between cattle-yaks and their parents[J].Anim Reprod Sci, 2011, 126(3-4):258-264.
[34]	LI T T, WANG X, ZHANG H Y, et al.Gene expression patterns and protein cellular localization suggest a novel role for DAZL in developing Tibetan sheep testes[J].Gene, 2020, 731:144335.
[35]	KIM K H, KANG Y J, JO J O, et al.DDX4(DEAD box polypeptide 4) colocalizes with cancer stem cell marker CD133 in ovarian cancers[J].Biochem Biophys Res Commun, 2014, 447(2):315-322.
[36]	HICKFORD D E, FRANKENBERG S, PASK A J, et al.DDX4(VASA) is conserved in germ cell development in marsupials and monotremes[J].Biol Reprod, 2011, 85(4):733-743.
[37]	FUJIWARA Y, KOMIYA T, KAWABATA H, et al.Isolation of a DEAD-family protein gene that encodes a murine homolog of Drosophila vasa and its specific expression in germ cell lineage[J].Proc Natl Acad Sci U S A, 1994, 91(25):12258-12262.
[38]	杨术环, 郭丹.辽宁绒山羊资源保护与品种选育[J].中国畜牧业, 2014(21):25-26.YANG S H, GUO D.Resource protection and breeding of liaoning cashmere goat[J].China Animal Industry, 2014(21):25-26.(in Chinese)
[39]	张帆.辽宁绒山羊品种资源保护与利用研究[D].北京:中国农业科学院, 2013.ZHANG F.The research of conservation and utility of Liaoning cashmere goats resource[D].Beijing:Chinese Academy of Agricultural Sciences, 2013.(in Chinese)
[40]	吕永锋, 任茂源, 郭彬彬, 等.舍饲陇东绒山羊同期发情及繁殖性状的研究[J].中国草食动物科学, 2018, 38(6):71-72.LV Y F, REN M Y, GUO B B, et al.Study on synchronous estrus and reproductive traits of Longdong Cashmere goats[J].China Herbivore Science, 2018, 38(6):71-72.(in Chinese)
[41]	杨军祥, 王珂, 郭海龙, 等.陇东黑山羊的现状及保护利用[J].畜牧兽医杂志, 2010, 29(6):55-57.YANG J X, WANG K, GUO H L, et al.Discussion on germplasm conservation and utilization of Longdong black goat[J].Journal of Animal Science and Veterinary Medicine, 2010, 29(6):55-57.(in Chinese)

Comparation of the Testes Development between Liaoning Cashmere Goat and Ziwuling Black Goat at Sexual Maturity

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