犏牛和牦牛ESCO2基因的序列特征及其在睾丸中的表达对比分析

doi:10.11843/j.issn.0366-6964.2021.011.014

Abstract

Abstract: The aim of this study was to clone the establishment of sister chromatid cohesion N-acetyltransferase 2(ESCO2) gene, analyze its expression and localization in the testis at different developmental stages in cattle-yak and yak, and provide a theoretical basis for further study the role mechanism of ESCO2 in the male meiosis process. Healthy male cattle-yaks and yaks were used as experimental animals in this study, they were divided into fetal cattle group (5-6 months old), juvenile group (1-2 years old) and adult group (3-4 years old), with 3 animals in each group. The CDS region of ESCO2 gene of cattle-yak and yak were cloned by RT-PCR and its sequence characteristics were analyzed by bioinformatics softwares. qRT-PCR was used to detect the expression patterns of ESCO2 gene in different tissues of cattle-yak. The expression of ESCO2 mRNA in testes at different developmental stages of cattle-yak and yak were detected by qRT-PCR, the differences of cell location and expression of ESCO2 protein were detected by immunohistochemistry (IHC) staining. The results showed that the CDS region of ESCO2 genes in cattle-yak was 1 833 bp(MW198470), it encoded 610 amino acids. Compared with yak, the ESCO2 protein sequence of cattle-yak had more 19 amino acids at position 301-319 aa and there were 3 amino acid mutations. The cattle-yak and cattle had higher homology than other mammals based on the protein sequence of ESCO2. Interaction network showed that ESCO2 mainly interacted with SMC3, SMC1A, PDS5A, PDS5B, STAG2 and these proteins were related to sister chromatid condensation, meiotic cell cycle, DNA repair, cell division and chromosome remodeling. ESCO2 was expressed in all tissues of the cattle-yak, but its relative expression level in the testis was significantly higher than those in other tissues (P<0.05). In addition, the spatial expression of ESCO2 in testes of cattle-yak showed an upward trend with age, and the relative expression abundance of ESCO2 in cattle-yak at juvenile and adult stages was significantly lower than that of yak(P<0.05). IHC staining showed that meiosis of male cattle-yak was blocked in primary spermatocytes and ESCO2 protein was differentially expressed in primary spermatocytes between cattle-yak and yak. The research suggested that there was obvious difference in the sequence and expression pattern of ESCO2 between cattle-yak and yak, which may be one of reasons caused male sterility in cattle-yak, but the specific mechanism need further study.

Key words: cattle-yak, ESCO2, testis, meiosis, gene expression

CLC Number:

S823.85

MIN Xingyu, YANG Lixue, YANG Manzhen, HU Yulei, YU Hailing, YANG Luyu, LI Jian, XIONG Xianrong. Sequence Characteristics and Comparative Expression Analysis in Testis of ESCO2 Gene in Cattle-yak and Yak[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3126-3136.

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Sequence Characteristics and Comparative Expression Analysis in Testis of ESCO2 Gene in Cattle-yak and Yak

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