组学技术分析肉鸡胚胎发育过程中肝脏蛋白表达的变化

doi:10.11843/j.issn.0366-6964.2020.02.006

Abstract

Abstract: This study aimed to explore the changes of protein levels in chicken embryos during the middle and late developmental stages. One hundred and twenty fertilized eggs with (65±0.2)g were selected and randomly divided into 2 groups (E14 and H1), 3 replicates per group, and 20 eggs in each replicate. The liver tissues were collected at E14 and H1, respectively. Proteomics approach based on isobaric tags for relative and absolute quantitation (iTRAQ) was employed to screen the core differential proteins in liver. The results showed that 10 core differential proteins with different expression levels were identified in H1 compared with E14, these proteins mainly enhanced fatty acid degradation (upregulated ACOX1, ACSL1, ACSL5, CPT1A and ECI2),and gluconeogenesis (upregulated ALDH3A2, FBP1, FBP2, GPI and PGM2,downregulated LDHB and ALDH9A1). These results indicate that the main energy supply pathway for embryo development is fatty acid degradation, not glycolysis. In addition, increased gluconeogenesis promotes glycogen storage to cope with shelling and changes in the nutritional environment.

Key words: chicken embryo, liver, metabolism, proteomics

CLC Number:

S831.2

PENG Mengling, HU Wenye, LI Naixin, WANG Juhua, DING Jianping, ZHOU Jie. The Changes of Hepatic Proteins during Chicken Embryonic Development Based on Proteomics Analysis[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(2): 252-259.

References 30

[1]	JEFFERIES B,LENZE F,SATHE A,et al.Non-invasive imaging of engineered human tumors in the living chicken embryo[J].Sci Rep,2017,7:4991.
[2]	ULOZA V,KUZMINIENE·A,PALUBINSKIENE·J,et al.Model of human recurrent respiratory papilloma on chicken embryo chorioallantoic membrane for tumor angiogenesis research[J].Histol Histopathol,2017,32(7):699-710.
[3]	李云雷,薛夫光,徐松山,等.产蛋后期母鸡脂肪沉积量与繁殖性能相关性研究[J].畜牧兽医学报,2018,49(6):1163-1168.L Y L,XUE F G,XU S S,et al.Relationship between fat deposition and reproduction performance of beijing-you chickens during the late stage of reproductive period[J].Acta Veterinaria et Zootechnica Sinica,2018,49(6):1163-1168.(in Chinese)
[4]	GARCIA K E,OKAMOTO R J,BAYLY P V,et al.Contraction and stress-dependent growth shape the forebrain of the early chicken embryo[J].J Mechan Beh Biomed Mater,2017,65:383-397.
[5]	马友彪,张海军,王晶,等.胚蛋给养β-羟基-β-甲基丁酸对肉仔鸡孵化率、生长性能和骨骼肌发育的影响[J].畜牧兽医学报,2016,47(11):2248-2256.M Y B,ZHANG H J,WANG J,et al.Effect of in ovo feeding of β-hydroxy-β-methylbutyrate on hatchability,performance and skeletal muscle development in broilers[J].Acta Veterinaria et Zootechnica Sinica,2016,47(11):2248-2256.(in Chinese)
[6]	SIEIRO D,VÉRON N,MARCELLE C.The chicken embryo as an efficient model to test the function of muscle fusion genes in amniotes[J].PLoS One,2017,12(5):e0177681.
[7]	TOO H C,SHIBATA M,YAYOTA M,et al.Expression of thyroid hormone regulator genes in the yolk sac membrane of the developing chicken embryo[J].J Reproduct Dev,2017,63(5):463-472.
[8]	李吕木, PEEBLES D.孵化期间鸡胚、肝脏、卵黄及蛋白组成的变化及其相关关系[J].畜牧兽医学报,2005,36(6):574-577.L L M,PEEBLES D.Chick embryo,liver,yolk and albumen compositional changes and their relationships during incubation[J].Acta Veterinaria et Zootechnica Sinica,2005,36(6):574-577.(in Chinese)
[9]	吴雪嘉,李乙江.鸡脂肪肝综合征营养防控[J].中国畜禽种业,2017,13(6):147.W X J,LI Y J.Nutritional control of chicken fatty liver syndrome[J].The Chinese Livestock and Poultry Breeding,2017,13(6):147.(in Chinese)
[10]	SA R N,XING H,LUAN S J,et al.Atmospheric ammonia alters lipid metabolism-related genes in the livers of broilers (Gallus gallus)[J]. Anim Physiol Anim Nutrit,2018,102(2):e941-e947.
[11]	徐梦飞,卢平萍,马勋,等.细粒棘球绦虫蛋白质组学研究进展[J].畜牧兽医学报,2018,49(3):466-476.X M F, LU P P, MA X, et al. Advances in Echinococcus granulosus proteomics[J]. Acta Veterinaria et Zootechnica Sinica, 2018, 49(3):466-476. (in Chinese)
[12]	熊火,蔡欣,刘静波,等.高脂饲粮对生长育肥猪肉品质和骨骼肌蛋白质组的影响[J].畜牧兽医学报,2016,47(10):2052-2059.XONG H,CAI X,LIU J B,et al.Effects of high-fat diet on meat quality traits and skeletal muscle proteome of growing-finishing pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2016,47(10):2052-2059.(in Chinese)
[13]	SOTILLO J,PEARSON M S,LOUKAS A.Trematode genomics and proteomics[M]//TOLEDO R,FRIED B.Digenetic Trematodes.Cham:Springer,2019,1154:411-436.
[14]	赵畅,张江,白云龙,等.基于iTRAQ技术的卵巢静止奶牛血清差异蛋白分析[J].畜牧兽医学报,2019,50(5):972-982.ZAO C,ZHANG J,BAI Y L,et al.Analysis of serum differential proteins in cows with inactive ovaries based on iTRAQ technology[J].Acta Veterinaria et Zootechnica Sinica,2019,50(5):972-982.(in Chinese)
[15]	KIM E T,ROCHE K L,KULEJ K,et al.SAMHD1 Modulates early steps during human cytomegalovirus infection by limiting NF-κB activation[J].Cell Rep,2019,28(2):434-448.e6.
[16]	潘洪彬,赵素梅,孙泽威,等.脂肪特异蛋白27(Fsp27)与脂肪代谢[J].畜牧兽医学报,2014,45(3):347-353.PN H B,ZHAO S M,SUN Z W,et al.Fat-specific protein 27(Fsp27) and lipid metabolism[J].Acta Veterinaria et Zootechnica Sinica,2014,45(3):347-353.(in Chinese)
[17]	IVESSA N E,REHBERG E,KIENZLE B,et al.Molecular cloning,expression,and hormonal regulation of the chicken microsomal triglyceride transfer protein[J].Gene,2013,523(1):1-9.
[18]	潘志雄,吕佳,卢立志,等.鹅ACSL1基因克隆及其在鹅肥肝形成中作用的初步研究[J].畜牧兽医学报,2010,41(11):1407-1413.PN Z X,LV J,LU L Z,et al.Cloning of goose ACSL1 gene,tissues expression and the effect of overfeeding on its mRNA level[J].Acta Veterinaria et Zootechnica Sinica,2010,41(11):1407-1413.(in Chinese)
[19]	TIAN W H,WANG Z,YUE Y X,et al.miR-34a-5p increases hepatic triglycerides and total cholesterol levels by regulating ACSL1 protein expression in laying hens[J].Int J Mol Sci,2019,20(18):4420.
[20]	DRUYAN S,RUZAL M,SHINDER D,et al.Effects of low oxygen during chorioallantoic membrane development on post-hatch growing performance of broiler chickens[J].Poult Sci,2018,97(6):1961-1967.
[21]	CUI M,WANG Y,SUN B D,et al.MiR-205 modulates abnormal lipid metabolism of hepatoma cells via targeting acyl-CoA synthetase long-chain family member 1(ACSL1) mRNA[J].Biochem Biophys Res Commun,2014,444(2):270-275.
[22]	TOBITA H,SATO S,YAZAKI T,et al.Alogliptin alleviates hepatic steatosis in a mouse model of nonalcoholic fatty liver disease by promoting CPT1a expression via Thr172 phosphorylation of AMPKα in the liver[J].Mol Med Rep,2018,17(5):6840-6846.
[23]	MAHAJAN A,CONSTANTINOU J,SIDIROPOULOS C.ECHS1 deficiency-associated paroxysmal exercise-induced dyskinesias:case presentation and initial benefit of intervention[J].J Neurol,2017,264(1):185-187.
[24]	DAN C,ZHANG H,ZENG W,et al.HNF1B expression regulates ECI2 gene expression,potentially serving a role in prostate cancer progression[J].Oncol Lett,2019,17(1):1094-1100.
[25]	LIAO C C,CHEN S C,HUANG H P,et al.Gallic acid inhibits bladder cancer cell proliferation and migration via regulating fatty acid synthase(FAS)[J].J Food Drug Anal,2018,26(2):620-627.
[26]	LI G X,FU S Y,CHEN Y,et al.MicroRNA-15a regulates the differentiation of intramuscular preadipocytes by targeting ACAA1,ACOX1 and SCP2 in chickens[J].Int J Mol Sci,2019,20(16):4063.
[27]	张凤,曾倩晖,陶虎,等.牛ACOX1基因过表达载体构建及对脂肪沉积的初步研究[J].中国畜牧杂志,2017,53(11):42-47.ZANG F,ZENG Q H,TAO H,et al.Construction of over-expression vector of bovine ACOX1 gene and preliminary study on fat deposition[J].Chinese Journal of Animal Science,2017,53(11):42-47.(in Chinese)
[28]	VASILIOU V,NEBERT D W.Analysis and update of the human aldehyde dehydrogenase (ALDH) gene family[J].HumGenomics,2005,2(2):138-143.
[29]	ROTHMAN D L,MAGNUSSON I,KATZ L D,et al.Quantitation of hepatic glycogenolysis and gluconeogenesis in fasting humans with 13C NMR[J].Science,1991,254(5031):573-576.
[30]	LI S N,YANG Z M,ZHANG H H,et al.(-)-hydroxycitric acid influenced fat metabolism via modulating of glucose-6-phosphate isomerase expression in chicken embryos[J].J Agric Food Chem,2019,67(26):7336-7347.

The Changes of Hepatic Proteins during Chicken Embryonic Development Based on Proteomics Analysis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 30

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Yili, TANG Jiao, MIN Qi, YANG Lu, WANG Zening, HU Lian, ZHAO Di, JIANG Mingfeng. Mining Key Candidate Genes of Development and Metabolism in Yak Abomasum Based on Transcriptome Data [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 153-168.
[2]	YAO Ying, ZHOU Yingcong, DU Peiyan, LI Yijuan, QIAN Wenjie, LI Liuyang, YU Zhipeng, CUI Yan, YU Sijiu, FAN Jiangfeng. Proteomic Analysis of Yak Serum During Pregnancy Based on TMT Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 192-206.
[3]	ZHANG De'an, YANG Ruozhu, LIU Jie, LIU Dewu, DENG Ming, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Expression Analysis of Transcriptome in the Liver of Chuanzhong Black Goats Fed with Silage Neolamarckia Cadamba Substitute for Silage Corn [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 232-244.
[4]	CHEN Yuetong, LIU Xiaohan, WANG Zhiyang, ZHAO Yuxin, ZHOU Tiezhong, HU Zengjin, ZHU Yue, WANG Shaohui, TIAN Mingxing, DING Siyu, QI Jingjing, YU Shengqing. Isolation, Identification, Pathogenicity and Drug Susceptibility of Mycoplasma gallisepticum from Dead Chicken Embryos in Large-scale Chicken Farms in Guangdong Province [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 290-299.
[5]	MA Yajun, JIAO Zhihui, LIU Xiaoning, LU Xiangyu, LIU Tao, WANG Yue, PIAO Chenxi, WANG Hongbin. Effects of Adipose-derived Mesenchymal Stem Cells on Pyroptosis of Miniature Pigs with Hepatic Ischemia-Reperfusion Combined with Hepatectomy [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 355-364.
[6]	ZHANG Yafeng, ZHU Bin, MA Chang, ZHANG Yuanshu. The Research on the Effects of ACE2 Activated by Diminazene Aceturate on Mitochondria in the Liver of Rats with Non-alcoholic Fatty Liver Disease [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3895-3904.
[7]	YUAN Heling, FANG Ci, HUANG Jinhu, WANG Xiaoming, XIAO Wenjun, LIU Ruiting, SHI Rongmei, WANG Liping. Effects of Allicin on mRNA Expression of CYP1A2 and Its Enzyme Kinetics in Broilers Liver Tissue [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3905-3915.
[8]	ZHENG Gang, LIAN Ling. Research Progress of Key Regulatory Gene DMRT1 in Chicken Sex Determination and Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3152-3163.
[9]	WU Zhili, YAO Junhu, LEI Xinjian. Research Progress of Rumen-protected Glucose on Nutritional Regulation in Perinatal Dairy Animals [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3173-3182.
[10]	CHEN Xin, QIN Tong. mRNA Vaccine and Its Research Prospect in Zoonotic Diseases [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2732-2742.
[11]	ZHAO Yangfei, YU Yanghuan, WANG Jinming, ZHANG Jianhai, SUN Zilong, NIU Ruiyan, WANG Jundong. Effects of IL-17A Knockout on Fluoride-Induced Hepatic Inflammation and Hepatocyte Apoptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3108-3117.
[12]	MAO Peng, WANG Zhihao, LI Jianji, CUI Luying, ZHU Guoqiang, MENG Xia, DONG Junsheng, WANG Heng. Research Progress of Ferroptosis in Bacterial Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2280-2287.
[13]	SHAO Yuexin, ZHANG Xinyu, GE Liyan, SHI Huaiping. Cloning and RNA Interference Analysis of ATF4 Gene in Xinong Saanen Dairy Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2353-2364.
[14]	ZHU Qian, CHENG Yating, LI Ruixuan, LI Chenjian, LIU Yating, KONG Xiangfeng. Effects of Probiotics and Synbiotics Addition to Sows’ Diet on Fatty Acid Composition and Related Gene Expression in Muscle of Offspring Bama Mini-Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2458-2467.
[15]	ZHAO Donghao, YUAN Meng, MA Kaiteng, DUAN Zhuo, ZHU Yixin, TANG Fang, HAN Keguang, HUO Nairui. Chelating Role of Sheep Bone Collagen Peptide to Cadmium and Its Protection Role against Liver Injuries Induced by Cadmium in Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2641-2652.