组学技术分析肉鸡胚胎发育过程中肝脏蛋白表达的变化

doi:10.11843/j.issn.0366-6964.2020.02.006

Abstract

Abstract: This study aimed to explore the changes of protein levels in chicken embryos during the middle and late developmental stages. One hundred and twenty fertilized eggs with (65±0.2)g were selected and randomly divided into 2 groups (E14 and H1), 3 replicates per group, and 20 eggs in each replicate. The liver tissues were collected at E14 and H1, respectively. Proteomics approach based on isobaric tags for relative and absolute quantitation (iTRAQ) was employed to screen the core differential proteins in liver. The results showed that 10 core differential proteins with different expression levels were identified in H1 compared with E14, these proteins mainly enhanced fatty acid degradation (upregulated ACOX1, ACSL1, ACSL5, CPT1A and ECI2),and gluconeogenesis (upregulated ALDH3A2, FBP1, FBP2, GPI and PGM2,downregulated LDHB and ALDH9A1). These results indicate that the main energy supply pathway for embryo development is fatty acid degradation, not glycolysis. In addition, increased gluconeogenesis promotes glycogen storage to cope with shelling and changes in the nutritional environment.

Key words: chicken embryo, liver, metabolism, proteomics

CLC Number:

S831.2

PENG Mengling, HU Wenye, LI Naixin, WANG Juhua, DING Jianping, ZHOU Jie. The Changes of Hepatic Proteins during Chicken Embryonic Development Based on Proteomics Analysis[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(2): 252-259.

References 30

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The Changes of Hepatic Proteins during Chicken Embryonic Development Based on Proteomics Analysis

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