鸡MOGAT和DGAT基因的克隆及表达特性研究

doi:10.11843/j.issn.0366-6964.2017.02.004

Abstract

Abstract:

The objectives of the present study were to clone the genes in MOGAT and DGAT families and characterize their expression and regulation properties in chicken. The coding sequences of genes including MOGAT1, MOGAT2, LPGAT1, DGAT2 and SOAT1 which encoded proteins with enzymatic activities of MOGAT and DGAT families were cloned and sequenced. The tissue distribution of the genes was investigated by RT-PCR, and the expression patterns of genes in liver between pre-laying and peak-laying hens were detected by real-time PCR. In addition, the expression and regulation mechanism of the genes were explored using both in vivo and in vitro models treated with estrogen. The results showed that the genes in MOGAT and DGAT families expressed extensively in chicken various tissues, and had relatively higher expression levels in the lipid metabolism organs such as liver, kidney, small intestine (duodenum, jejunum, ileum), the DGAT2 gene had lower expression in liver and the MOGAT2 gene was mainly expressed in small intestine (duodenum, jejunum, ileum). MOGAT1 was expressed in the most of tissues except pancreas. The expression levels of MOGAT1, LPGAT1 and SOAT1 genes in liver of peak-laying hens were significantly lower than that in pre-laying hens. The expression levels of the genes in liver, duodenum, kidney and primary hepatocyte maintained no change or significantly decreased after the chickens and primary hepatocytes were treated with estrogen, respectively. In conclusion, the known genes in MOGAT and DGAT families, which play important roles in mammalian TG metabolism, are not the key genes in TG synthesis in liver of chicken, and the monoacylglycerol pathway don't play a leading role in chicken TG synthesis metabolism induced by estrogen.

CLC Number:

S831.2

TIAN Fang-yuan, WANG Tai-an, LI Yan-min, LI Cui-cui, MA Zheng, KANG Xiang-tao, TIAN Ya-dong, LIU Xiao-jun. Cloning and Expression Characteristics of MOGAT and DGAT Genes in Chicken (Gallus gallus)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 214-224.

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Cloning and Expression Characteristics of MOGAT and DGAT Genes in Chicken (Gallus gallus)

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