黄芪多糖和甲砜霉素影响ALV-J 对SPF鸡致病性的分析

doi:10.11843/j.issn.0366-6964.2015.10.020

Abstract

Abstract:

In order to further verify the influence of early application of astragalan and thiamphenicol on the ALV-J congenital infection，5-day-old-SPF chickens were inoculated with 1000 TCID₅₀ ALV-J through the yolk sac and count the mortality of the SPF embryos during incubation.Then，105 SPF chickens at 1 day of age were randomly assigned into three groups of 35 each.The experimental group was respectively given with astragalan(100 mg•L^-1) and thiamphenicol(100 mg•L^-1) lasting for five days，three times with 5 days of the internal，and the last group was the virus infected control.A comprehensive evaluation about astragalan and thiamphenicol on the influence of ALV-J congenital infection was carried out by cloaca detoxification and viremia of ALV-J，vaccine immunization level of ND，the immune organ index and body weight，tumor incidence and histopathological examination.The results indicated that the two drugs had no significant influence on viremia elimination，occurrence of ALV-J antibody and the increase of ND antibody.Similarly，the weight difference between astragalus group and virus infected group was not significant at the whole experimental process；Although early weight of thiamphenicol group was obviously higher than that of other two groups，its weight was lower than that of other two groups after 60 d.The significant deduction of peripheral blood leucocyte/lymphocyte at 6 d and immune organ index of the spleen and bursa of Fabricius at 42 d in thiamphenicol group was observed lower than those of other two groups.Furthermore，tumors incidence at thiamphenicol group was the highest，28.57%(10/35)，followed by infection control group’s 20%(7/35) and astragalus group’s 11.43%(4/35)，and tumor types also appeared diversification including the hemangioma，myelocytomas，lymphatic sarcoma，fibrosarcoma and adenocarcinoma.Therefore，the early application of thiamphenicol can lead to immunosuppression，promote the tumorigenic effect and diversify the tumor types of ALV-J.Although the astragalus can reduce the pathogenicity of ALV-J，it can’t contribute to the elimination of viremia.

CLC Number:

S859.7

WANG Guang-wen，Lü Lin，WANG Chao，ZHANG Zhen-dong，CHENG Zi-long，QU Ya-jin，LIU Si-dang. Analysis of Astragalan and Thiamphenicol Affecting ALV-J Pathogenicity on SPF Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1858-1866.

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Analysis of Astragalan and Thiamphenicol Affecting ALV-J Pathogenicity on SPF Chicken

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