福建省猪群感染牛病毒性腹泻病毒的病原学检测与分析

doi:10.11843/j.issn.0366-6964.2014.12.014

Abstract

Abstract:

The study was aimed at establishing a reverse transcriptase PCR (RT-PCR) to differentiate swine bovine viral diarrhea virus (BVDV) and classical swine fever virus (CSFV) for the investigation of BVDV from swine in Fujian province and combined infection of swine BVDV.According to the conserved gene sequence of BVDV 5′-UTR，a specific RT-PCR was established and then sensitivity，specificity，repeatability were tested for the detection of BVDV in 458 clinical samples from 42 swine farms in 9 regions of Fujian province.Then according to the reference CSFV，porcine reproductive and respiratory syndrome virus (PRRSV)，highly pathogenic PRRSV (HP-PRRSV)，pseudorabies virus (PRV) and porcine circovirus type 2 (PCV-2) were detected in BVDV positive samples.The results showed that sensitivity was determined as 0.1 TCID₅₀ BVDV.In addition，all negative controls such as CSFV，PRRSV，HP-PRRSV，PRV，PCV-2 and porcine parvovirus virus (PPV) showed negative detection in the specificity assay.Both sensitivity and specificity were repeated five times with similar results.The BVDV positive rate of swine，swine farm and 9 regions was 10.04% (46/458)，78.57% (33/42) and 100% (9/9) in samples by sequence data，respectively，and the positive rate of 46 BVDV positive samples which infected with CSFV，PRRSV，HP-PRRSV，PRV and PCV-2 were 91.30% (42/46)，50.00% (23/46)，41.30% (19/46)，63.04% (29/46) and 37.00% (17/46).These findings indicated that the method with high sensitivity，specificity and reproducibility could provide an efficient tool for detection of swine BVDV.The BVDV infection in swine was common in Fujian province.And it was combined infection with other pathogens， especially CSFV.

CLC Number:

S852.659.6

XU Lei，ZENG Liang-ming,WANG Yu-ling，CHEN Xian-jin，LIN Bo-quan，LIN Gong-yang，FU Guang-hua，SHI Shao-hua，CHENG Long-fei，HUANG Yu，ZHANG Yuan-kui. Investigation of Bovine Viral Diarrhea Virus (BVDV) from Swine in Fujian Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 2006-2012.

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Investigation of Bovine Viral Diarrhea Virus (BVDV) from Swine in Fujian Province

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