畜牧兽医学报 ›› 2026, Vol. 57 ›› Issue (1): 270-281.doi: 10.11843/j.issn.0366-6964.2026.01.023

• 生物技术与繁殖 • 上一篇    下一篇

鸭精液品质相关睾丸代谢物的差异分析

朱春红1(), 陶志云1, 耿明阳3, 王志成1, 宋卫涛1, 顾昊天1, 刘宏祥1, 章双杰1, 陈黎2, 徐文娟1, 王逸飞1, 李慧芳1()   

  1. 1.江苏省家禽科学研究所,扬州 225125
    2.浙江省农业科学院,杭州 310009
    3.伊犁哈萨克自治州畜牧总站,伊犁哈萨克自治州畜牧科学研究所,伊犁 835000
  • 收稿日期:2025-06-04 出版日期:2026-01-23 发布日期:2026-01-26
  • 通讯作者: 李慧芳 E-mail:zhuch_1304428@126.com;lhfxf_002@aliyun.com
  • 作者简介:朱春红,博士生,研究员,主要从事家禽遗传资源评价与保护研究,E-mail:zhuch_1304428@126.com
  • 基金资助:
    国家重点研发计划资助(2022YFD1300100);江苏省“种业振兴”揭榜挂帅项目(JBGS(2021)111);“天池英才”引进计划;国家家养动物种质资源库;扬州市现代农业(YZ2024054)

Differential Analysis of Testicular Metabolites Associated with Semen Quality in Ducks

ZHU Chunhong1(), TAO Zhiyun1, GENG Mingyang3, WANG Zhicheng1, SONG Weitao1, GU Haotian1, LIU Hongxiang1, ZHANG Shuangjie1, CHEN Li2, XU Wenjuan1, WANG Yifei1, LI Huifang1()   

  1. 1.Jiangsu Institute of Poultry Science,Yangzhou 225125,China
    2.Zhejiang Academy of Agricultural Sciences,Hangzhou 310009,China
    3.Yili Kazakh Autonomous Prefecture Livestock Station,Yili Kazakh Autonomous Prefecture Institute of Animal Science,Yili 835000,China
  • Received:2025-06-04 Online:2026-01-23 Published:2026-01-26
  • Contact: LI Huifang E-mail:zhuch_1304428@126.com;lhfxf_002@aliyun.com

摘要:

旨在从代谢组学角度分析鸭精液品质相关的睾丸组织代谢机制。本研究选择精液品质高低差异显著的公鸭各6只,共12只,采集睾丸组织,使用超高效液相色谱串联质谱(UPLC-MS/MS)技术分析不同组别睾丸组织代谢物差异,通过富集差异代谢物筛选睾丸精液品质相关的潜在关键通路。在不同精液品质的睾丸组织中共检测到98个差异代谢物,其中显著上调15个,显著下调83个。差异代谢物主要包括脂肪酰类、氨基酸及其代谢物、甘油磷脂类、核苷酸及其代谢物、苯及其衍生物、辅酶和维生素、激素及激素相关物质等类别。差异代谢物之间相关性分析发现,脂肪酰类和苯及其衍生物呈最大正相关;碳水化合物及其代谢物和醛、酮、酯类呈最大负相关。精液品质指标与差异代谢物相关性分析发现,脂肪酰类、甘油磷脂类等多种代谢物与精液活力、精子动态参数呈显著正/负相关,|R|≥0.80。对差异代谢物进行富集分析,筛选出2条潜在的重要代谢通路,分别为脂肪酸降解(P=0.000 59)和甘油磷脂代谢(P=0.012 76)。本试验从代谢组学角度揭示了鸭不同精液品质睾丸的差异代谢物和潜在代谢机制,筛选出以脂质代谢物(脂肪酸类、甘油磷脂类)为主导的98个差异代谢物,其中精液品质高组肉碱类显著下调而十六烷酸和麦芽糖醇富集,并锁定脂肪酸降解和甘油磷脂代谢两条核心通路与精液品质显著关联,为家禽睾丸功能研究提供了关键代谢物和通路靶点。

关键词: 鸭, 睾丸, 代谢组学, 差异代谢物, 代谢通路

Abstract:

This study aimed to analyze the metabolic mechanisms of testicular tissue associated with semen quality in ducks from a metabolomics perspective. Semen quality was evaluated in a duck population. Twelve drakes (6 with significantly high semen quality and 6 with significantly low semen quality) were selected, and testicular tissues were collected. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to analyze differences in testicular metabolites between groups. Enrichment analysis of differential metabolites was performed to screen potential key pathways related to testicular semen quality. A total of 98 differentially abundant metabolites was identified in testicular tissues of ducks with varying semen quality, including 15 significantly upregulated and 83 significantly downregulated metabolites. These metabolites primarily belonged to categories such as fatty acyls, amino acids and their metabolites, glycerophospholipids, nucleotides and their metabolites, benzene derivatives, coenzymes and vitamins, and hormones. Correlation analysis revealed the strongest positive correlation between fatty acyls and benzene derivatives, while the strongest negative correlation was observed between carbohydrates/their metabolites and aldehydes/ketones/esters. Semen quality parameters (e.g., sperm motility and kinematic parameters) showed significant positive/negative correlations (|R|≥0.80) with fatty acyls, glycerophospholipids, and other metabolites. Enrichment analysis highlighted two critical metabolic pathways: fatty acid degradation (P=0.000 59) and glycerophospholipid metabolism (P=0.012 76). This study elucidated differential metabolites and potential metabolic mechanisms associated with semen quality in duck testicular tissues through metabolomics. It identifies 98 differential metabolites dominated by lipids (fatty acyls and glycerophospholipids), characterized by significant downregulation of carnitines and enrichment of hexadecanoic acid (FFA16:0) and maltitol in the high semen quality group, and identifies fatty acid degradation and glycerophospholipid metabolism as core pathways significantly associated with semen quality. These findings provide key metabolites and pathway targets for research on avian testicular function.

Key words: duck, testis, metabolomics, differential metabolites, metabolic pathways

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