一株新型鸭呼肠孤病毒的分离鉴定及其σC基因序列分析

doi:10.11843/j.issn.0366-6964.2025.05.046

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (5): 2520-2524.doi: 10.11843/j.issn.0366-6964.2025.05.046

• 研究简报 • 上一篇

一株新型鸭呼肠孤病毒的分离鉴定及其σC基因序列分析

李婷(), 张成成, 王秀玲, 张小荣, 吴艳涛*()

扬州大学兽医学院江苏高校动物重要疫病与人兽共患病防控协同创新中心, 扬州 225009

收稿日期:2024-05-22 出版日期:2025-05-23 发布日期:2025-05-27
通讯作者: 吴艳涛 E-mail:3310855423@qq.com;ytwu@yzu.edu.cn
作者简介:李婷(1999-)，女，四川威远人，硕士生，主要从事畜禽病原学与免疫学研究，E-mail: 3310855423@qq.com
基金资助:
财政部和农业农村部：国家现代农业产业技术体系(CARS-40)资助；江苏高校优势学科建设工程资助项目

Isolation and Identification of a Novel Duck Reovirus Strain and the Sequence Analysis of Its σC Gene

LI Ting(), ZHANG Chengcheng, WANG Xiuling, ZHANG Xiaorong, WU Yantao*()

Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China

Received:2024-05-22 Online:2025-05-23 Published:2025-05-27
Contact: WU Yantao E-mail:3310855423@qq.com;ytwu@yzu.edu.cn

摘要/Abstract

摘要：

旨在研究安徽地区新型鸭呼肠孤病毒(NDRV)的流行现状及遗传变异情况。本研究从安徽一养殖场患有脾梗死的麻鸭中分离出1株NDRV，命名为AH812。通过RT-PCR、鸭胚分离鉴定及动物致病性试验，结合σC基因序列分析，结果显示：分离的病毒为NDRV，纯净性试验显示，其他常见病原(鸭肝炎病毒、鸭坦布苏病毒、番鸭细小病毒、腺病毒与鸭圆环病毒)均为阴性；致病性试验通过腿部肌肉途径注射0.5ml分离病毒AH812(TCID₅₀=10^-4.25/0.1 mL)，雏鸭出现脾肿大、出血等典型症状。序列分析表明，分离株AH812与2020年山东分离株NDRV-N7(登录号：MW030529.1)亲缘关系最近。综上，本研究成功分离得到1株NDRV病毒AH812，对麻鸭有较强的致病性。

关键词: 新型鸭呼肠孤病毒, 分离, 鉴定, σC基因, 遗传进化分析

Abstract:

The aim of this study was to investigate the epidemiological status and genetic variation of novel duck reovirus (NDRV) in the Anhui region. A strain of NDRV was isolated from a flock of muscovy ducks exhibiting splenic infarction at a farm in Anhui Province. The strain was designated as AH812. Identification was conducted through RT-PCR, duck embryo isolation, pathogenicity assays, and σC gene sequence analysis. The results indicated that the isolated virus was confirmed as NDRV. Purity tests showed negative results for other common pathogens, including duck hepatitis virus, duck Tembusu virus, Muscovy duck parvovirus, adenovirus, and duck circovirus.Pathogenicity tests were performed by intramuscular injection of 0.5 mL of the AH812 isolate (TCID50=10^-4.25/0.1 mL) into ducklings leg muscle. The inoculated ducklings exhibited typical symptoms, such as splenomegaly and hemorrhage. Sequence analysis revealed that the AH812 strain shares the closest phylogenetic relationship with the 2020 NDRV isolate N7 from Shandong Province (GenBank accession number: MW030529.1).In conclusion, this study successfully isolated a novel NDRV strain, AH812, which demonstrates significant pathogenicity in muscovy ducks.

Key words: novel duck reovirus, isolation, identification, σC gene, genetic evolution analysis

中图分类号:

S852.659.4

李婷, 张成成, 王秀玲, 张小荣, 吴艳涛. 一株新型鸭呼肠孤病毒的分离鉴定及其σC基因序列分析[J]. 畜牧兽医学报, 2025, 56(5): 2520-2524.

LI Ting, ZHANG Chengcheng, WANG Xiuling, ZHANG Xiaorong, WU Yantao. Isolation and Identification of a Novel Duck Reovirus Strain and the Sequence Analysis of Its σC Gene[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(5): 2520-2524.

图/表 3

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一株新型鸭呼肠孤病毒的分离鉴定及其σC基因序列分析

Isolation and Identification of a Novel Duck Reovirus Strain and the Sequence Analysis of Its σC Gene

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