

畜牧兽医学报 ›› 2026, Vol. 57 ›› Issue (1): 270-281.doi: 10.11843/j.issn.0366-6964.2026.01.023
朱春红1(
), 陶志云1, 耿明阳3, 王志成1, 宋卫涛1, 顾昊天1, 刘宏祥1, 章双杰1, 陈黎2, 徐文娟1, 王逸飞1, 李慧芳1(
)
收稿日期:2025-06-04
出版日期:2026-01-23
发布日期:2026-01-26
通讯作者:
李慧芳
E-mail:zhuch_1304428@126.com;lhfxf_002@aliyun.com
作者简介:朱春红,博士生,研究员,主要从事家禽遗传资源评价与保护研究,E-mail:zhuch_1304428@126.com
基金资助:
ZHU Chunhong1(
), TAO Zhiyun1, GENG Mingyang3, WANG Zhicheng1, SONG Weitao1, GU Haotian1, LIU Hongxiang1, ZHANG Shuangjie1, CHEN Li2, XU Wenjuan1, WANG Yifei1, LI Huifang1(
)
Received:2025-06-04
Online:2026-01-23
Published:2026-01-26
Contact:
LI Huifang
E-mail:zhuch_1304428@126.com;lhfxf_002@aliyun.com
摘要:
旨在从代谢组学角度分析鸭精液品质相关的睾丸组织代谢机制。本研究选择精液品质高低差异显著的公鸭各6只,共12只,采集睾丸组织,使用超高效液相色谱串联质谱(UPLC-MS/MS)技术分析不同组别睾丸组织代谢物差异,通过富集差异代谢物筛选睾丸精液品质相关的潜在关键通路。在不同精液品质的睾丸组织中共检测到98个差异代谢物,其中显著上调15个,显著下调83个。差异代谢物主要包括脂肪酰类、氨基酸及其代谢物、甘油磷脂类、核苷酸及其代谢物、苯及其衍生物、辅酶和维生素、激素及激素相关物质等类别。差异代谢物之间相关性分析发现,脂肪酰类和苯及其衍生物呈最大正相关;碳水化合物及其代谢物和醛、酮、酯类呈最大负相关。精液品质指标与差异代谢物相关性分析发现,脂肪酰类、甘油磷脂类等多种代谢物与精液活力、精子动态参数呈显著正/负相关,|R|≥0.80。对差异代谢物进行富集分析,筛选出2条潜在的重要代谢通路,分别为脂肪酸降解(P=0.000 59)和甘油磷脂代谢(P=0.012 76)。本试验从代谢组学角度揭示了鸭不同精液品质睾丸的差异代谢物和潜在代谢机制,筛选出以脂质代谢物(脂肪酸类、甘油磷脂类)为主导的98个差异代谢物,其中精液品质高组肉碱类显著下调而十六烷酸和麦芽糖醇富集,并锁定脂肪酸降解和甘油磷脂代谢两条核心通路与精液品质显著关联,为家禽睾丸功能研究提供了关键代谢物和通路靶点。
中图分类号:
朱春红, 陶志云, 耿明阳, 王志成, 宋卫涛, 顾昊天, 刘宏祥, 章双杰, 陈黎, 徐文娟, 王逸飞, 李慧芳. 鸭精液品质相关睾丸代谢物的差异分析[J]. 畜牧兽医学报, 2026, 57(1): 270-281.
ZHU Chunhong, TAO Zhiyun, GENG Mingyang, WANG Zhicheng, SONG Weitao, GU Haotian, LIU Hongxiang, ZHANG Shuangjie, CHEN Li, XU Wenjuan, WANG Yifei, LI Huifang. Differential Analysis of Testicular Metabolites Associated with Semen Quality in Ducks[J]. Acta Veterinaria et Zootechnica Sinica, 2026, 57(1): 270-281.
表1
公鸭体重与精子活力指标比较"
分组 Group | 体重/g Body weight | 活动精子数/个 Motile sperm count | 精子活力/% Sperm motility | 精子活率/% Sperm viability | 精子死亡率/% Sperm mortality rate |
|---|---|---|---|---|---|
精液质量低组G_L组 Low semen quality group (G_L Group) | 1 397.125±141.50 | 490.88±134.32 | 71.23±7.79 | 81.60±5.27 | 18.40±5.27 |
精液质量高组G_H组 High semen quality group (G_H Group) | 1 343.00±109.75 | 892.69±131.53 | 98.21±0.33 | 99.64±0.07 | 0.37±0.07 |
| P值 P-value | 0.408 00 | 0.000 03 | 0.000 02 | 0.000 03 | 0.000 03 |
表2
公鸭精子动态参数比较"
分组 Group | 直线速率/(µm·s-1) Straight-linevelocity | 曲线速率/(µm·s-1) Curvilinearvelocity | 路径速度/(µm·s-1) Pathvelocity | 侧摆幅值/µm Amplitude of lateral head displacement | 摆动性 Wobble | 线性度 Linearity | 移动角度 Movement angle | 前向性 Straightness |
|---|---|---|---|---|---|---|---|---|
精液质量低组G_L组 Low semen qualitygroup (G_L Group) | 21.35± 3.34 | 44.23± 4.96 | 31.27±3.51 | 12.95± 1.45 | 0.85±0.05 | 0.49±0.05 | 389.00±6.28 | 0.69±0.06 |
精液质量高组G_H组 High semen qualitygroup (G_H Group) | 28.51± 6.58 | 66.07± 6.92 | 46.72±4.90 | 19.35± 2.03 | 0.96±0.03 | 0.42±0.04 | 909.14±137.89 | 0.60±0.05 |
P值 P-value | 0.020 10 | 0.000 01 | 0.000 01 | 0.000 01 | 0.000 33 | 0.007 41 | 0.000 00 | 0.006 63 |
表3
前30种显著差异代谢物"
物质一级分类 Primary classificationof substances | 物质 Substance | 化合物 Compound | 化学式 Formula | VIP | P-value | log2FC |
|---|---|---|---|---|---|---|
脂肪酰类 Fatty acyl classes | 二十二碳七烯酸 | FFA(22:7) | C22H30O2 | 2.332 6 | 0.000 1 | -0.382 8 |
| 肉碱 C20:3 | Carnitine C20:3 | C27H47NO4 | 2.302 8 | 0.000 8 | -0.830 4 | |
| 十六烷酸 | FFA(16:0) | C16H32O2 | 2.240 7 | 0.003 6 | 0.849 6 | |
| 肉碱 C24:5 | Carnitine C24:5 | C31H51NO4 | 2.102 2 | 0.004 9 | -0.755 5 | |
| 肉碱 C18:0 | Carnitine C18:0 | C25H49NO4 | 2.088 7 | 0.003 3 | -0.684 9 | |
| 肉碱 C22:4 | Carnitine C22:4 | C29H49NO4 | 2.067 7 | 0.001 2 | -0.814 3 | |
| 肉碱 C20:2 | Carnitine C202 | C27H49NO4 | 2.058 5 | 0.010 9 | -0.831 1 | |
| 肉碱 C20:1-OH | Carnitine C20:1-OH | C27H51NO5 | 2.027 8 | 0.029 1 | -1.113 0 | |
| 肉碱 C20:1 | Carnitine C20:1 | C27H51NO4 | 2.026 5 | 0.003 5 | -0.737 8 | |
| 肉碱 C18-OH | Carnitine C18-OH | C25H49NO5 | 2.022 0 | 0.023 7 | -0.946 0 | |
| 肉碱 C17:0 | Carnitine C17:0 | C24H47NO4 | 2.009 5 | 0.008 3 | -0.807 7 | |
| 肉碱 C18:2 | Carnitine C18:2 | C25H45NO4 | 2.000 4 | 0.021 3 | -0.760 5 | |
氨基酸及其代谢物 Amino acids andmetabolites | L-赖氨酸-L-苏氨酸 | Lys-Thr | C10H21N3O4 | 2.572 6 | 0.000 1 | -2.908 2 |
| 脯氨酸-亮氨酸 | Pro-Leu | C11H20N2O3 | 2.019 9 | 0.004 2 | -0.574 9 | |
苯及其衍生物 Benzene and derivatives | 4-甲氧基肉桂酸乙酯 | Ethyl-4-Methoxycinnamate | C12H14O3 | 2.276 6 | 0.000 3 | -0.273 4 |
| 2,6-二叔丁基-1,4-苯醌 | 2,6-di-tert-butyl-1,4-benzoquinone | C14H20O2 | 2.071 8 | 0.001 6 | -1.189 6 | |
醇、胺类 Alcohols and amines classes | 十六碳酰胺 | Hexadecanamide | C16H33NO | 2.376 8 | 0.000 3 | -1.847 5 |
胆汁酸 Bile acids | 猪去氧胆酸 | Hyodeoxycholic acid | C24H40O4 | 2.294 3 | 0.000 5 | -1.051 6 |
辅酶和维生素 Coenzymes and vitamins | 反式-13,14-二羟视黄醇 (Z)-古尔司酮 | All-Trans-13,14-Dihydroretinol (Z)-Guggulsterone | C20H32O | 2.470 5 | 0.015 7 | -3.156 6 |
| C21H28O2 | 2.186 4 | 0.000 5 | -0.464 7 | |||
甘油磷脂类 Glycerophospholipid classes | 溶血磷脂酰丝氨酸_ LPS(20:1) | LPS(20:1) | C26H50NO9P | 2.566 4 | 0.000 2 | -2.930 1 |
| LPE(O-18:2) | LPE(O-18:2) | C23H46NO6P | 2.227 2 | 0.000 3 | -0.543 6 | |
| LPC(O-16:1) | LPC(O-16:1) | C24H50NO6P | 2.193 5 | 0.001 3 | -0.758 7 | |
核苷酸及其代谢物 Nucleotides and metabolites | 5'-腺苷酸硫酸(APS) | 5'-Adenylyl sulfate (APS) | C10H14N5O10PS | 2.074 2 | 0.004 1 | -0.465 5 |
激素及激素相关物质 Hormones and hormone-related substances | 7-酮胆固醇 | 7-Ketocholesterol | C27H44O2 | 2.462 3 | 0.000 0 | -1.295 0 |
| 20,26-二羟基蜕皮激素 | 20,26-dihydroxyecdysone | C27H44O8 | 2.134 8 | 0.004 0 | -0.587 9 | |
鞘脂类 Sphingolipid classes | 鞘氨醇_SPH (d18:0) | SPH(d18:0) | C18H39NO2 | 2.043 7 | 0.008 7 | 0.510 7 |
醛、酮、酯类 Aldehydes, ketones and esters classes | 2-戊基-3-苯基-2-丙烯醛 | 2-Pentyl-3-phenyl-2-propenal | C14H18O | 2.496 8 | 0.000 0 | -0.870 9 |
碳水化合物及代谢物 Carbohydrates and metabolites | 麦芽糖醇 | Maltitol | C12H24O11 | 2.573 8 | 0.000 2 | 2.863 4 |
有机酸及其衍生物 Organic acids and derivatives | 2-羟基己二酸 | 2-Hydroxyadipic acid | C6H10O5 | 2.315 0 | 0.000 3 | 0.306 4 |
| [1] | 张 博,唐 静,赵 睿,等.种母鸭核黄素缺乏对子代雏鸭初生重、器官指数、体尺指标及血浆生化指标的影响[J].动物营养学报,2020,32(5):2185-2191. |
| ZHANG B,TANG J,ZHAO R,et al.Effects of riboflavin deficiency in breeder ducks on birth weight,organ index,body size indexes and plasma biochemical indexes of offspring ducklings[J].Chinese Journal of Animal Nutrition,2020,32(5):2185-2191.(in Chinese) | |
| [2] | 解广娟.公鸭精液品质及精浆蛋白组学的研究[D].雅安:四川农业大学,2022. |
| XIE G J.Study on semen quality and seminal plasma proteomics of male ducks[D].Ya'an:Sichuan Agricultural University,2022.(in Chinese) | |
| [3] | GRISWOLD M D.The central role of Sertoli cells in spermatogenesis[J].Semin Cell Dev Biol,1998,9(4):411-416. |
| [4] | 李金梅.雄激素通过间接调控PLZF介导的精原干细胞分化机制研究[D].南京:南京农业大学,2016. |
| LI J M.Study on the mechanism of androgens indirectly regulating PLZF-mediated spermatogonial stem cell differentiation[D].Nanjing:Nanjing Agricultural University,2016.(in Chinese) | |
| [5] | 彭红波,刘 晃,宋小燕,等.代谢组学技术对非梗阻性无精子症的诊断价值[J].检验医学与临床,2023,20(5):625-629. |
| PENG H B,LIU H,SONG X Y,et al.Diagnostic value of metabolomics technology in non-obstructive azoospermia[J].Laboratory Medicine and Clinic,2023,20(5):625-629.(in Chinese) | |
| [6] | ZHU Z,LI R,FENG C,et al.Exogenous oleic acid and palmitic acid improve boar sperm motility via enhancing mitochondrial beta-oxidation for ATP generation[J].Animals (Basel),2020,10(4):591. |
| [7] | KARAHALIL B.Overview of systems biology and omics technologies[J].Curr Med Chem,2016,23(37):4221-4230. |
| [8] | ZHAO Q,HUANG J F,CHENG Y,et al.Polyamine metabolism links gut microbiota and testicular dysfunction[J].Microbiome,2021,9(1):224. |
| [9] | SETIAWAN R,PRIYADARSHANA C,MIYAZAKI H,et al.Functional difference of ATP-generating pathways in rooster sperm (Gallus gallus domesticus)[J].Anim Reprod Sci,2021,233:106843. |
| [10] | 赵延辉,陈 余,王 梁,等.基于RNA-seq数据筛选鸡睾丸附睾间差异表达基因及其功能分析[J].中国畜牧杂志,2022,58(1):117-122. |
| ZHAO Y H,CHEN Y,WANG L,et al.Screening of differentially expressed genes between testis and epididymis of chickens based on RNA-seq data and their functional analysis[J].Chinese Journal of Animal Science,2022,58(1):117-122.(in Chinese) | |
| [11] | KUANG W,ZHANG J,LAN Z,et al.SLC22A14 is a mitochondrial riboflavin transporter required for sperm oxidative phosphorylation and male fertility[J].Cell Rep,2021,35(3):109025. |
| [12] | 赵志显,常雪蕊,郭 勇,等.种公鸡精液品质营养调控的研究进展[J].畜牧兽医学报,2022,53(8):2435-2443. |
| ZHAO Z X,CHANG X R,GUO Y,et al.Research progress on nutritional regulation of breeder rooster semen quality[J].Acta Veterinaria et Zootechnica Sinica,2022,53(8):2435-2443.(in Chinese) | |
| [13] | JERYSZ A,LUKASZEWICZ E.Effect of dietary selenium and vitamin E on ganders' response to semen collection and ejaculate characteristics[J].Biol Trace Elem Res,2013,153(1-3):196-204. |
| [14] | WANG Y,FU X,LI H.Mechanisms of oxidative stress-induced sperm dysfunction[J].Front Endocrinol (Lausanne),2025,16:1520835. |
| [15] | NICHOLSON J K,LINDON J C,HOLMES E.'Metabonomics':understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J].Xenobiotica,1999,29(11):1181-1189. |
| [16] | HAN G,HONG S H,LEE S J,et al.Transcriptome analysis of testicular aging in mice[J].Cells,2021,10(11):2895. |
| [17] | PATTI G J,YANES O,SIUZDAK G.Innovation:Metabolomics:the apogee of the omics trilogy[J].Nat Rev Mol Cell Biol,2012,13(4):263-269. |
| [18] | YANG K,XIA B,WANG W,et al.A comprehensive analysis of metabolomics and transcriptomics in cervical cancer[J].Sci Rep,2017,7:43353. |
| [19] | WANG Y T,YANG Y,SUN X L,et al.Development of a widely-targeted metabolomics method based on gas chromatography-mass spectrometry[J].Se Pu,2023,41(6):520-526. |
| [20] | STOCKL J B,SCHMID N,FLENKENTHALER F,et al.Proteomic insights into senescence of testicular peritubular cells from a nonhuman primate model[J].Cells,2020,9(11):2498. |
| [21] | ALPAUGH W F,VOIGT A L,DARDARI R,et al.Loss of ubiquitin carboxy-terminal hydrolase L1 impairs long-term differentiation competence and metabolic regulation in murine spermatogonial stem cells[J].Cells,2021,10(9):2265. |
| [22] | CAI X,WU S,MIPAM T,et al.Testis transcriptome profiling identified lncRNAs involved in spermatogenic arrest of cattleyak[J].Funct Integr Genomics,2021,21(5-6):665-678. |
| [23] | O'DONNELL L,REBOURCET D,DAGLEY L F,et al.Sperm proteins and cancer-testis antigens are released by the seminiferous tubules in mice and men[J].FASEB J,2021,35(3):e21397. |
| [24] | WENK M R.Lipidomics:new tools and applications[J].Cell,2010,143(6):888-895. |
| [25] | IUCHI K,TAKAI T,HISATOMI H.Cell Death via lipid peroxidation and protein aggregation diseases[J].Biology (Basel),2021,10(5):399. |
| [26] | LEE W K,LAM T K Y,TANG H C,et al.PFOS-elicited metabolic perturbation in liver and fatty acid metabolites in testis of adult mice[J].Front Endocrinol (Lausanne),2023,14:1302965. |
| [27] | 覃佐剑.基于LC-MS/MS氧化脂肪酸数据库的构建和应用[D].北京:中国农业科学院,2020. |
| QIN Z J.Construction and application of oxidized fatty acid database based on LC-MS/MS[D].Beijing:Chinese Academy of Agricultural Sciences,2020.(in Chinese) | |
| [28] | 陈嘉康,吕春荣,苗永旺,等.脂质组学技术在家畜精液质量评价和体外保存研究中的应用研究进展[J].中国畜牧兽医,2022,49(12):4697-4706. |
| CHEN J K,LV C R,MIAO Y W,et al.Research progress on the application of lipidomics technology in livestock semen quality evaluation and in vitro preservation[J].China Animal Husbandry & Veterinary Medicine,2022,49(12):4697-4706.(in Chinese) | |
| [29] | FLESCH F M,GADELLA B M.Dynamics of the mammalian sperm plasma membrane in the process of fertilization[J].Biochim Biophys Acta,2000,1469(3):197-235. |
| [30] | 胡学春.脂肪酸对小鼠睾丸支持细胞存活率的影响及机制研究[D].南京:南京大学,2018. |
| HU X C.Study on the effect and mechanism of fatty acids on the survival rate of mouse testicular Sertoli cells[D].Nanjing:Nanjing University,2018.(in Chinese) | |
| [31] | ILICETO M,STENSEN M H,ANDERSEN J M,et al.Levels of L-carnitine in human seminal plasma are associated with sperm fatty acid composition[J].Asian J Androl,2022,24(5):451-457. |
| [32] | ARSLAN E,KOYUNCU I,TEMIZ E,et al.Comparison of sperm carnitine profiles of normospermic,oligospermic and azospermic individuals[J].Eur Rev Med Pharmacol Sci,2023,27(17):8154-8162. |
| [33] | MATEUS F G,MOREIRA S,MARTINS A D,et al.L-carnitine and male fertility:Is supplementation beneficial?[J].J Clin Med,2023,12(18):5796. |
| [34] | VAN MEER G,VOELKER D R,FEIGENSON G W.Membrane lipids:where they are and how they behave[J].Nat Rev Mol Cell Biol,2008,9(2):112-124. |
| [35] | 张宇霆.猪精子差异耐冻性机制与耐冻性生物标志物筛选[D].哈尔滨:东北农业大学,2021. |
| ZHANG Y T.Mechanism of differential freezing tolerance of boar sperm and screening of freezing tolerance biomarkers[D].Harbin:Northeast Agricultural University,2021.(in Chinese) | |
| [36] | ENGEL K M,BAUMANN S,ROLLE-KAMPCZYK U,et al.Metabolomic profiling reveals correlations between spermiogram parameters and the metabolites present in human spermatozoa and seminal plasma[J].PLoS One,2019,14(2):e0211679. |
| [37] | LI Y,HU Y,WANG Z,et al.IKBA phosphorylation governs human sperm motility through ACC-mediated fatty acid beta-oxidation[J].Commun Biol,2023,6(1):323. |
| [38] | 何慧慧,刘卫红,贾 瑞,等.当归芍药散对PM2.5致雄性生殖损伤大鼠睾丸组织代谢组学的影响[J].郑州大学学报(医学版),2022,57(1):38-44. |
| HE H H,LIU W H,JIA R,et al.Effect of Danggui Shaoyao Powder on metabolomics of testicular tissue in rats with male reproductive injury induced by PM2.5[J].Journal of Zhengzhou University (Medical Sciences),2022,57(1):38-44.(in Chinese) | |
| [39] | AM-IN N,KIRKWOOD R N,TECHAKUMPHU M,et al.Lipid profiles of sperm and seminal plasma from boars having normal or low sperm motility[J].Theriogenology,2011,75(5):897-903. |
| [40] | 陈佳颖,马博闻,赵青余,等.公猪精液质量与精子脂质成分相关性研究[J].中国畜牧兽医,2024,51(4):1537-1548. |
| CHEN J Y,MA B W,ZHAO Q Y,et al.Study on the correlation between boar semen quality and sperm lipid components[J].China Animal Husbandry & Veterinary Medicine,2024,51(4):1537-1548.(in Chinese) |
| [1] | 李晨阳, 张传美, 惠宇韬, 吕莹, 杨敏. 15种血清型的鸭疫里默氏杆菌毒力基因检测和耐药性分析[J]. 畜牧兽医学报, 2026, 57(1): 360-368. |
| [2] | 王雅君, 刘奇, 王扬扬, 何雷, 魏颖, 陈松彪, 余祖华, 丁轲, 陈建. EAV-HP在SLCO1B3基因5’端非编码区整合影响芦花鸡肝脏代谢组学研究[J]. 畜牧兽医学报, 2026, 57(1): 476-485. |
| [3] | 董佳宁, 胡樱凡, 窦宇飞, 李俊, 石磊, 任有蛇. 高硒通过PI3K/AKT/FoxO1信号通路对绵羊睾丸间质细胞自噬的影响[J]. 畜牧兽医学报, 2025, 56(8): 3837-3848. |
| [4] | 屠民航, 蔡根谭, 宋彦泽, 安贵花, 吴姜衡, 马龙飞, 施振旦, 韩国锋, 陈哲, 王恬, 王超. 枸杞黄酮通过调控Nrf2/HO-1信号通路改善肉鸭肌肉品质[J]. 畜牧兽医学报, 2025, 56(8): 3922-3932. |
| [5] | 陈柳, 相生瑞, 云涛, 倪征, 华炯钢, 朱寅初, 张存, 叶伟成. 鸭瘟病毒疫苗株UL35缺失株的构建及其免疫保护效果评价[J]. 畜牧兽医学报, 2025, 56(8): 3933-3941. |
| [6] | 郑浩, 罗芳, 宋承磊, 陶金忠. 基于代谢组学技术筛选人工授精后未妊娠奶牛血浆潜在生物标志物的研究[J]. 畜牧兽医学报, 2025, 56(7): 3252-3264. |
| [7] | 霍振, 庄蕾, 周伟, 王帅钦, 谢明, 侯水生, 唐静. 叶酸对1~21日龄北京鸭生产性能、血浆生化指标和抗氧化能力的影响[J]. 畜牧兽医学报, 2025, 56(7): 3327-3334. |
| [8] | 张虔, 马睿, 崔燕, 余四九. AMPK/SIRT1介导脂联素促进牦牛睾丸支持细胞乳酸转运的分子机制[J]. 畜牧兽医学报, 2025, 56(7): 3495-3506. |
| [9] | 朱爱文, 王健, 朱戈辉, 刘海霞, 平措班旦, 王军, 德庆卓嘎, 闫伟, 韩大勇. 玉米赤霉烯酮致彭波半细毛羊睾丸支持细胞增殖凋亡、氧化应激及NAC保护机制[J]. 畜牧兽医学报, 2025, 56(6): 2752-2764. |
| [10] | 李婷, 张成成, 王秀玲, 张小荣, 吴艳涛. 一株新型鸭呼肠孤病毒的分离鉴定及其σC基因序列分析[J]. 畜牧兽医学报, 2025, 56(5): 2520-2524. |
| [11] | 孟祥旭, 李佳, 任德明, 陈奎蓉, 和艺云, 王立贤, 盛熙晖, 王立刚. 民猪猪繁殖与呼吸综合征恢复力高低组血清代谢组学研究[J]. 畜牧兽医学报, 2025, 56(4): 1689-1699. |
| [12] | 叶润根, 刘渊博, 路丽丽, Collins Amponsah Asiamah, 苏瑛. miR-215-5p在雷州黑鸭组织中的表达及其对卵泡颗粒细胞增殖和凋亡的影响[J]. 畜牧兽医学报, 2025, 56(4): 1722-1730. |
| [13] | 王昕昕, 刘小英, 王宜, 王芳, 赵晗, 杜志强, 杨彩侠. 急性热应激通过降低牛磺酸水平影响猪睾丸支持细胞的功能[J]. 畜牧兽医学报, 2025, 56(4): 1779-1790. |
| [14] | 于江玮, 程慧敏, 林健, 杨宝琳, 黄程, 杨志远, 胡格. 鸭瘟病毒TaqMan荧光定量PCR检测方法的建立及应用[J]. 畜牧兽医学报, 2025, 56(2): 765-773. |
| [15] | 吴佳辉, 沈世彦, 邓锦波, 吴海阳, 任芷欣, 吴杨博, 黄娟, 黄浩滨, 潘伟雄, 赵锃珏, 何容肖, 孙崇军, 张玲华. 诱导型表达H5N1亚型禽流感病毒HA蛋白的乳酸乳球菌的构建及其对鸭的免疫原性分析[J]. 畜牧兽医学报, 2025, 56(2): 774-787. |
| 阅读次数 | ||||||
|
全文 |
|
|||||
|
摘要 |
|
|||||