十足目虹彩病毒病诊断方法的研究进展

doi:10.11843/j.issn.0366-6964.2025.04.008

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (4): 1549-1560.doi: 10.11843/j.issn.0366-6964.2025.04.008

十足目虹彩病毒病诊断方法的研究进展

邢静怡¹^,²(), 郭晓萌²^,³, 王蒙²^,⁴, 管鑫²^,⁴, 邱亮²^,⁴^,*(), 李安琪¹^,*(), 张庆利¹^,²^,³^,⁴, 黄倢²^,³

1. 天津农学院水产学院, 天津 300392
2. 海水养殖生物育种与可持续产出全国重点实验室, 青岛海洋科技中心海洋渔业科学与食物产出过程功能实验室, 农业农村部海水养殖病害防治重点实验室, 青岛市海水养殖流行病学与生物安保重点实验室(中国水产科学研究院黄海水产研究所)青岛 266071
3. 上海海洋大学水产与生命学院, 上海 201306
4. 鲁东大学农学院, 烟台 264011

收稿日期:2024-06-12 出版日期:2025-04-23 发布日期:2025-04-28
通讯作者: 邱亮,李安琪 E-mail:xing-jingyi@foxmail.com;qiuliang@ysfri.ac.cn;anqi826523329@163.com
作者简介:邢静怡(1998-), 女, 河南安阳人, 硕士生, 主要从事虾蟹疫病诊断研究, E-mail: xing-jingyi@foxmail.com
基金资助:
国家重点研发计划项目(2023YFD2402200)；中国-东盟海水养殖技术“一带一路”联合实验室建设与海水养殖技术联合研究项目(2022YFE0203900)；山东省自然科学基金项目(ZR2021QC144)；中国水产科学研究院基本科研业务费(2023TD42)；黄海水产研究所基本科研业务费项目(20603022024019)

Advances in Diagnostic Methods of Infection with Decapod Iridescent Virus 1

XING Jingyi¹^,²(), GUO Xiaomeng²^,³, WANG Meng²^,⁴, GUAN Xin²^,⁴, QIU Liang²^,⁴^,*(), LI Anqi¹^,*(), ZHANG Qingli¹^,²^,³^,⁴, HUANG Jie²^,³

1. College of Aquaculture, Tianjin Agricultural University, Tianjin 300392, China
2. State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Key Laboratory of Maricultural Organism Disease Control of Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences), Qingdao 266071, China
3. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
4. School of Agriculture, Ludong University, Yantai 264011, China

Received:2024-06-12 Online:2025-04-23 Published:2025-04-28
Contact: QIU Liang, LI Anqi E-mail:xing-jingyi@foxmail.com;qiuliang@ysfri.ac.cn;anqi826523329@163.com

摘要/Abstract

摘要：

十足目虹彩病毒病是一种新发的甲壳动物疫病，由十足目虹彩病毒1(Decapod iridescent virus 1，DIV1)感染引起。该病毒包含两个原始分离株，即虾血细胞虹彩病毒(shrimp hemocyte iridescent virus，SHIV 20141215)和红螯螯虾虹彩病毒(Cherax quadricarinatus iridovirus，CQIV CN01)。DIV1传播速度快、宿主范围广、致死率高，在近年来广泛流行于虾蟹养殖业中，给产业造成巨大的经济损失。目前，针对此疫病已开发了多种诊断技术，本文对此进行了总结，以期为十足目虹彩病毒病的准确诊断和防控提供参考。

关键词: 十足目虹彩病毒1(DIV1), 虾血细胞虹彩病毒(SHIV), 红螯螯虾虹彩病毒(CQIV), 疫病诊断, 甲壳动物

Abstract:

Infection with Decapod iridescent virus 1 (DIV1) is a newly emerged crustacean viral disease caused by DIV1. The virus consists of two original isolates, namely shrimp hemocyte iridescent virus (SHIV 20141215) and Cherax quadricarinatus iridovirus (CQIV CN01). DIV1 is fast spreading, with a wide host range and high lethality, and it has become widely prevalent in shrimp and crab aquaculture in recent years, causing significant economic losses to the industry. Currently, various diagnostic techniques have been developed for infection with DIV1. This paper provides a summary of these techniques, with the aim of providing reference for the accurate diagnosis, prevention and control of this disease.

Key words: decapod iridescent virus 1, shrimp hemocyte iridescent virus (SHIV), Cherax quadricarinatus iridovirus (CQIV), disease diagnosis, crustacea

中图分类号:

S852.659.1

邢静怡, 郭晓萌, 王蒙, 管鑫, 邱亮, 李安琪, 张庆利, 黄倢. 十足目虹彩病毒病诊断方法的研究进展[J]. 畜牧兽医学报, 2025, 56(4): 1549-1560.

XING Jingyi, GUO Xiaomeng, WANG Meng, GUAN Xin, QIU Liang, LI Anqi, ZHANG Qingli, HUANG Jie. Advances in Diagnostic Methods of Infection with Decapod Iridescent Virus 1[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(4): 1549-1560.

图/表 4

图 1

图 2

图 3

表 1

DIV1各种检测方法的对比"

诊断/检测技术 Diagnostic/ Testing technology	检测靶标 Detection target	定性/定量 Qualitative/ Quantitative	特异性 Specificity	WOAH试验验证所处阶段 Stage of WOAH test validation	最低检出限 Limit of detection (LOD)	弊端 Disadvantage	适用场景 Applicable Scenarios	检测温度 Detection temperature	参考文献 References
外观症状 Appearance symptom	-	定性	-	无	-	依赖专业人员	实验室/现场	-	-
组织病理学 Histopathology	-	定性	-	无	-	步骤繁琐、依赖专业人员	实验室	-	-
透射电镜 TEM	-	定性	-	无	-	人员	实验室	-	-
原位杂交检测技术(ISH) In situ hybridization	MCP	定性	-	无	-	步骤繁琐、依赖设备和人员	实验室	需要高温变性DNA	[4]
									[30]
原位地高辛标记的环介导等温扩增ISDL	DNA-依赖的RNA聚合酶Ⅱ的第二大亚基	定性	-	无	-	步骤繁琐、依赖设备和人员	实验室	需要高温变性DNA	[5]
									[30]
PCR	MCP	定性	-	无	-	依赖设备	实验室	变温	[3]
套式PCR Nested PCR	ATPase	定性	强	阶段1	36 fg样品总DNA	依赖设备	实验室	变温	[30]
半嵌套式PCR Semi-nested PCR	MCP ATPase	定性	强	阶段1 阶段1	1.0×10¹拷贝·反应^-1	依赖设备	实验室	变温	[9]
TaqMan qPCR	ATPase	定量	强	阶段2	4拷贝·反应^-1	依赖设备	实验室	变温	[31]
TaqMan qPCR	MCP	定量	强	阶段2	1.2拷贝·反应^-1	依赖设备	实验室	变温	[32]
TaqMan qPCR	11L	定量	强	阶段2	1.0拷贝·反应^-1	依赖设备	实验室	变温	[36]
TaqMan qPCR	ATPase	定量	强	阶段2	9.5×10¹拷贝·反应^-1	依赖设备	实验室	变温	[33]
SYBR Green qPCR	51R	定量	强	阶段2	3.1拷贝·反应^-1	依赖设备	实验室	变温	[37]
	105R			阶段2	2.2拷贝·反应^-1
	114R			阶段2	8.6拷贝·反应^-1
	124R			阶段2	2.9拷贝·反应^-1
	MCP	定量	强	阶段2^*	1.24×10²拷贝·反应^-1	依赖设备	实验室	变温	[38]
Eva Green qPCR	MCP	定量	强	阶段2	1.0×10²拷贝·反应^-1	容易因核酸染料非特异地与DNA结合，造成假阳性	实验室	变温	[39]
LAMP	MCP	定量	强	阶段2^*	3.54×10²拷贝·反应^-1	容易因非特异性扩增导致假阳性	实验室/现场	64.4 ℃	[34]
qLAMP	ATPase	定量	强	阶段2	3.8×10²拷贝·反应^-1	对目标DNA定量的准确性略低于qPCR	实验室/ 现场	63 ℃	[33]
LAMP、LAMP-dye、LAMP-LFD	DNA-依赖的RNA聚合酶Ⅱ的第二大亚基	定性	强	阶段1	3.95×10³拷贝·反应^-1	灵敏度较低	实验室/现场	60 ℃	[42]
LAMP Micro-detection Slide	ATPase	定性	强	阶段2	1.7×10²拷贝·反应^-1	可能出现假阴性结果	实验室/现场	65 ℃	[43]
RPA	ATPase	定性	强	阶段2^*	1.4×10¹拷贝·反应^-1	试剂成本高于qPCR	实验室/现场	30 ℃	[48]
RPA	ATPase	定性	强	阶段2	8拷贝·反应^-1	试剂成本高于qPCR	实验室/现场	37 ℃	[49]
Real-time RPA	MCP	定性	强	阶段1	1.1×10¹拷贝·反应^-1	试剂成本高于qPCR	实验室/现场	39 ℃	[35]
Real-time RPA	MCP	定性	强	阶段2^*	2.3×10¹拷贝·反应^-1	试剂成本高于qPCR	实验室/现场	39 ℃	[50]
qRPA	ATPase	定量	强	阶段1	2拷贝·反应^-1	试剂成本高于qPCR	实验室	42 ℃	[51]
DIV1-RPA-SYBR Green I		定性		阶段1	2.0×10³拷贝·反应^-1		实验室/现场
间接酶联免疫吸附试验(间接ELISA) Indirect ELISA	ORF064L	定量	强	阶段2	5 ng·mL^-1	步骤繁琐，成本较高	实验室/现场	常温	[53]
点印迹试验 Dot blot test				阶段2	6.25 ng·点^-1

表 1

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十足目虹彩病毒病诊断方法的研究进展

Advances in Diagnostic Methods of Infection with Decapod Iridescent Virus 1

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