马疱疹病毒1型分离毒株对叙利亚金黄地鼠的致病性

doi:10.11843/j.issn.0366-6964.2025.01.030

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (1): 327-334.doi: 10.11843/j.issn.0366-6964.2025.01.030

马疱疹病毒1型分离毒株对叙利亚金黄地鼠的致病性

刘建华¹^,²^,*(), 撒瑞雪¹^,²(), 张嗣玉¹^,², 李银涛¹^,², 邓智超¹^,², 贾晗铎¹^,², 赵敏¹^,², 付玉¹^,², 杨一明¹^,², 冉多良¹^,², 加尔肯¹^,²

1. 新疆农业大学动物医学学院动物传染病实验室, 乌鲁木齐 830052
2. 新疆农业大学动物医学学院新疆草食动物新药研究与创制重点实验室, 乌鲁木齐 830052

收稿日期:2024-03-11 出版日期:2025-01-23 发布日期:2025-01-18
通讯作者: 刘建华 E-mail:liujianhua627@126.com;1715019231@qq.com
作者简介:刘建华(1972-)，男，重庆人，副教授，硕士，主要从事动物传染病诊断与防治研究，E-mail: liujianhua627@126.comliujianhua627@126.com
撒瑞雪(1997-)，女，新疆乌鲁木齐人，回族，硕士生，主要从事动物传染病的诊断与防治研究，E-mail：1715019231@qq.com
第一联系人：
刘建华和撒瑞雪为同等贡献作者
基金资助:
中央引导地方科技发展专项资金项目(ZYYD2023C03)；新疆农业大学大学生创新训练计划项目(S202210758063)

Pathogenicity of Equine Herpesvirus Type 1 Isolates to Syrian Golden Hamsters

LIU Jianhua¹^,²^,*(), SA Ruixue¹^,²(), ZHANG Siyu¹^,², LI Yintao¹^,², DENG Zhichao¹^,², JIA Handuo¹^,², ZHAO Min¹^,², FU Yu¹^,², YANG Yiming¹^,², RAN Duoliang¹^,², JA Erken¹^,²

1. Laboratory of Animal Infectious Diseases, College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China
2. Xinjiang Key Laboratory of New Drug Research and Development for Herbivores, College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China

Received:2024-03-11 Online:2025-01-23 Published:2025-01-18
Contact: LIU Jianhua E-mail:liujianhua627@126.com;1715019231@qq.com

摘要/Abstract

摘要：

为了解从伊犁地区分离的EHV-1/China/YL2023的生物学特性及其对叙利亚金黄地鼠的致病性，采用蚀斑试验、病毒培养与增殖、饱和硫酸铵浓缩及蔗糖密度梯度离心等方法，对分离株进行纯化。使用不同剂量的EHV-1/China/YL2023病毒，以鼻内接种的方式感染叙利亚金黄地鼠，探究该分离株的致病效果。结果表明，纯化后的EHV-1/China/YL2023分离株病毒滴度达10^6.64 TCID₅₀·mL^-1。EHV-1/China/YL2023分离株感染4~5周龄叙利亚鼠后，14 d内的出现精神沉郁、食量减退、口部流涎、蜷缩、弓背、皮毛凌乱掉落及不同程度的神经症状。耐过地鼠的平均体重下降25.9%，10⁷~10⁵ TCID₅₀·0.1 mL^-1剂量组的存活率均为50%。病理结果显示，不同剂量组的病毒对叙利亚鼠的肺部脑部造成不同程度的损伤，即肺泡壁增厚，脑部神经元肿胀、大量中性粒细胞、淋巴细胞浸润，出血充血等。不同组织病毒载量测定结果显示，叙利亚鼠的肺、脑及淋巴结中均含有病毒DNA，且在肺和脑中的含量较高。综上所述，EHV-1/China/YL2023分离株对4~5周龄叙利亚金黄地鼠具有较高的致病性。本研究为EHV-1生物学特性研究及其致病机理提供依据，为后期解决EHV-1的流传及疫苗的研发问题提供支持。

关键词: 马疱疹病毒1型, 蚀斑纯化, 病毒增殖, 致病性

Abstract:

In order to understand the biological characteristics of EHV-1/China/YL2023 isolated from Yili and its pathogenicity to Syrian golden hamsters, the isolates were purified by plaque assay, virus culture and proliferation, saturated ammonium sulfate concentration and sucrose density gradient centrifugation. To investigate the pathogenicity of EHV-1/China/YL2023 virus isolate, Syrian golden hamsters were infected with different doses of EHV-1/China/YL2023 virus by intranasal inoculation. The results showed that the titer of purified EHV-1/China/YL2023 strain reached 10^6.64 TCID₅₀·mL^-1. Syrian mice aged 4-5 weeks infected with EHV-1/China/YL2023 strain showed depression, decreased food intake, drooling, curled up, hunched back, unkily dropped fur and varying degrees of neurological symptoms within 14 days. The average body weight of the recovered hamsters decreased by 25.9%, and the survival rate of the 10⁷-10⁵ TCID₅₀·0.1 mL^-1 dose groups was 50%.The pathological results showed that different doses of virus caused different degrees of damage to the lungs and brains of Syrian mice, including alveolar wall thickening, brain neuron swelling, a large number of neutrophils and lymphocytes infiltration, hemorrhage and congestion. The viral load measurement results of different tissues showed that the Syrian rats contained viral DNA in the lungs, brain and lymph nodes, and the content was high in the lungs and brain. In conclusion, the EHV-1/China/YL2023 isolates were highly pathogenic to Syrian golden hamsters aged 4 to 5 weeks. This study provides a basis for the study of the biological characteristics and pathogenic mechanism of EHV-1, and provides support for the future development of EHV-1 vaccine.

Key words: EHV-1, purification of plaque, multiplication of virus, pathogenicity

中图分类号:

刘建华, 撒瑞雪, 张嗣玉, 李银涛, 邓智超, 贾晗铎, 赵敏, 付玉, 杨一明, 冉多良, 加尔肯. 马疱疹病毒1型分离毒株对叙利亚金黄地鼠的致病性[J]. 畜牧兽医学报, 2025, 56(1): 327-334.

LIU Jianhua, SA Ruixue, ZHANG Siyu, LI Yintao, DENG Zhichao, JIA Handuo, ZHAO Min, FU Yu, YANG Yiming, RAN Duoliang, JA Erken. Pathogenicity of Equine Herpesvirus Type 1 Isolates to Syrian Golden Hamsters[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 327-334.

图/表 6

图 1

图 2

图 3

图 4

图 5

图 6

参考文献 16

1	MARENZONI M L , DE WAURE C , TIMONEY P J . Efficacy of vaccination against equine herpesvirus type 1 (EHV-1) infection: systematic review and meta-analysis of randomised controlled challenge trials[J]. Equine Vet J, 2023, 55 (3): 389- 404. doi: 10.1111/evj.13870
2	MAHMOUD H Y A H , ANDOH K , HATTORI S , et al. Characterization of glycoproteins in equine herpesvirus-1[J]. J Vet Med Sci, 2013, 75 (10): 1317- 1321. doi: 10.1292/jvms.13-0168
3	BARRANDEGUY M , VISSANI A , OLGUIN C , et al. Experimental reactivation of equine herpesvirus-3 following corticosteroid treatment[J]. Equine Vet J, 2008, 40 (6): 593- 595. doi: 10.2746/042516408X333399
4	NEHAL S S. Preliminary trials for the production of equine viral abortion inactivated vaccine[D]. Benha: Virology Benha University, 2006.
5	OLADUNNI F S , HOROHOV D W , CHAMBERS T M . EHV-1: A constant threat to the horse industry[J]. Front Microbiol, 2019, 10, 2668. doi: 10.3389/fmicb.2019.02668
6	MIAO J X , CHARD L S , WANG Z M , et al. Syrian hamster as an animal model for the study on infectious diseases[J]. Front Immunol, 2019, 10, 2329. doi: 10.3389/fimmu.2019.02329
7	车传忠, 鲍子磊, 胡月, 等. 马疱疹病毒1型感染马疾病模型的建立及评价[J]. 中国畜牧兽医, 2021, 48 (6): 2196- 2203.
	CHE C Z , BAO Z L , HU Y , et al. Establishment and evaluation of equine herpesvirus type 1 infection equine disease model[J]. China Animal Husbandry & Veterinary Medcine, 2021, 48 (6): 2196- 2203.
8	胡月, 刘建华, 王雪竹, 等. 马疱疹病毒1型TaqMan实时荧光定量PCR检测方法的建立[J]. 中国兽医学报, 2021, 41 (12): 2345- 2350.
	HU Y , LIU J H , WANG X Z , et al. Establishment of TaqMan real-time PCR for detecting equid Herpesvirus 1[J]. Chinese Journal of Veterinary Science, 2021, 41 (12): 2345- 2350.
9	杨永龙, 刘建华, 宋焕堂, 等. 新疆伊犁地区马疱疹病毒1型的分离与鉴定[J]. 中国预防兽医学报, 2016, 38 (7): 550- 553.
	YANG Y L , LIU J H , SONG H T , et al. Isolation and identification of equine herpesvirus 1 in Xinjiang[J]. Chinese Journal of Preventive Veterinary Medicine, 2016, 38 (7): 550- 553.
10	段汝丽, 佟盼盼, 邓海峰, 等. Ⅷ群神经致病型马疱疹病毒1型的分离及鉴定[J]. 中国预防兽医学报, 2022, 44 (9): 940- 945.
	DUAN R L , TONG P P , DENG H F , et al. Isolation and identification of neuropathogenic equid herpesvirus 1 within group Ⅷ[J]. Chinese Journal of Preventive Veterinary Medicine, 2022, 44 (9): 940- 945.
11	HUSSEY S B , CLARK R , LUNN K F , et al. Detection and quantification of equine herpesvirus-1 viremia and nasal shedding by real-time polymerase chain reaction[J]. J Vet Diagn Invest, 2006, 18 (4): 335- 342. doi: 10.1177/104063870601800403
12	PAILLOT R , SUTTON G , THIEULENT C , et al. New EHV-1 variant identified[J]. Vet Rec, 2020, 186 (17): 573.
13	BURTON E A , WECHUCK J B , WENDELL S K , et al. Multiple applications for replication-defective herpes simplex virus vectors[J]. Stem Cells, 2001, 19 (5): 358- 377. doi: 10.1634/stemcells.19-5-358
14	AWAN A R , CHONG Y C , FIELD H J . The pathogenesis of equine herpesvirus type 1 in the mouse: a new model for studying host responses to the infection[J]. J General Virol, 1990, 71 (5): 1131- 1140. doi: 10.1099/0022-1317-71-5-1131
15	KIM S K , SHAKYA A K , O'CALLAGHAN D J . Immunization with attenuated equine herpesvirus 1 strain KyA induces innate immune responses that protect mice from lethal challenge[J]. J Virol, 2016, 90 (18): 8090- 8104. doi: 10.1128/JVI.00986-16
16	SALEH A G , EL-HABASHI N , ABD-ELLATIEFF H A , et al. Comparative study of the pathogenesis of rhinopneumonitis induced by intranasal inoculation of hamsters with equine herpesvirus-9, equine herpesvirus-1 strain Ab4p and zebra-borne equine herpesvirus-1[J]. J Comp Pathol, 2020, 180, 35- 45. doi: 10.1016/j.jcpa.2020.08.002

[1]	范维, 刘昕昕, 翟艺禄, 张新玉, 王唯, 付佳棋, 孙福亮. 羊源肺炎克雷伯菌分离鉴定及其外膜囊泡提取方法的建立[J]. 畜牧兽医学报, 2025, 56(1): 353-364.
[2]	王浩, 王世玉, 肖金龙, 沈珏, 潘天灵, 张靖松, 肖鹏, 高洪. 猪源大肠杆菌高致病性毒力岛结构基因进化分析及其对TNF/NF-κB通路的影响[J]. 畜牧兽医学报, 2025, 56(1): 392-403.
[3]	张姗, 刘大虎, 刘宝京, 梁琳, 梁瑞英, 汤新明, 仇旭升, 丁铲, 丁家波, 侯绍华. 一株鸽副黏病毒Ⅰ型分离鉴定及致病性分析[J]. 畜牧兽医学报, 2024, 55(9): 4051-4060.
[4]	谢碧林, 林志敏, 林彬彬, 徐以娟, 林锋强, 闫露, 伍慧妮, 李翠婷, 周海欧, 李兆龙. 鸭疫里氏杆菌LC1和CX1株的分离鉴定及致病性分析[J]. 畜牧兽医学报, 2024, 55(9): 4196-4203.
[5]	王艳, 高亚东, 蒋成辉, 曾巧英. 一株鹅源禽腺病毒4型的分离及致病性[J]. 畜牧兽医学报, 2024, 55(9): 4232-4240.
[6]	张帆帆, 李杰茂, 谭佳, 黄江南, 吴玲, 韦启鹏, 康昭风. 禽偏肺病毒的研究进展[J]. 畜牧兽医学报, 2024, 55(8): 3344-3353.
[7]	孙雨点, 宋紫玥, 张洪亮, 秦志华, 单虎, 杨瑞梅. 鸭短喙与侏儒综合征病毒分离与鉴定[J]. 畜牧兽医学报, 2024, 55(8): 3623-3630.
[8]	刘维哲, 罗成刚, 袁蓉, 廖艺杰, 文艺悯, 孙莹, 俞恩波, 曹三杰, 黄小波. 一株猪流行性腹泻病毒强毒株的分离与鉴定[J]. 畜牧兽医学报, 2024, 55(7): 3049-3063.
[9]	班玛王清, 陈曦, 岳怡, 苏玉蓉, 岳华, 汤承. 一株牛呼吸道冠状病毒的分离鉴定及部分生物学特征[J]. 畜牧兽医学报, 2024, 55(7): 3094-3104.
[10]	刘博华, 符汉宇, 王玉恒, 索朗斯珠, 牛家强, 包玉花, 李家奎, 徐业芬. 西藏那曲市牦牛源B型多杀性巴氏杆菌的分离鉴定及基因组分析[J]. 畜牧兽医学报, 2024, 55(7): 3105-3118.
[11]	杨坤, 马静文, 周新瑞, 罗烈柱, 刘喆, 胡自强, 武星辰, 梁立滨, 高诗敏. 三株传染性法氏囊病病毒重组毒株的致病性研究[J]. 畜牧兽医学报, 2024, 55(6): 2550-2559.
[12]	周宁, 汤承, 许佳, 岳华, 陈曦. 猫泛白细胞减少症病毒A91S变异株对猫的致病性及基因组特征研究[J]. 畜牧兽医学报, 2024, 55(6): 2560-2568.
[13]	熊挺, 何献铭, 赵希雅, 庄婷婷, 黄美珍, 梁世金, 余传照, 梁雪静, 陈瑞爱. 三株鸡传染性支气管炎病毒优势流行毒株全基因组分析及其致病性[J]. 畜牧兽医学报, 2024, 55(5): 2109-2122.
[14]	郑芮, 刘紫石, 张康友, 颜勇, 魏玲, 泽仁翁姆, 丁则德米, 黄建钧, 王利, 魏勇. 花生茎源茉莉炭疽菌的分离鉴定及生物学特性研究[J]. 畜牧兽医学报, 2024, 55(5): 2206-2213.
[15]	秦祎, 胡文洁, 方小伟, 郭骞, 田篮鑫, 刘芳, 方春. 脂磷壁酸合成酶ltaS基因缺失对产单核细胞李氏杆菌致病性的影响[J]. 畜牧兽医学报, 2024, 55(2): 670-679.

马疱疹病毒1型分离毒株对叙利亚金黄地鼠的致病性

Pathogenicity of Equine Herpesvirus Type 1 Isolates to Syrian Golden Hamsters

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 16

相关文章 15

编辑推荐

Metrics

本文评价