奶牛早期妊娠诊断蛋白的研究

doi:10.11843/j.issn.0366-6964.2024.02.004

摘要/Abstract

摘要： 奶牛早期胚胎死亡导致的妊娠失败,可造成奶牛场巨大的经济损失。对未妊娠奶牛进行及早且准确的妊娠诊断,将提高奶牛妊娠效率,缩短空怀天数。本文综述了近年来国内外有关妊娠相关蛋白、奶牛早期妊娠阶段血液、尿液、乳液等体液蛋白及蛋白组学研究,以确认早期未妊娠的潜在蛋白,从而在妊娠早期对奶牛进行准确的妊娠诊断,提高奶牛的生产效益。

关键词: 奶牛, 蛋白组学, 早期妊娠诊断, 生物标志物, 妊娠相关物质

Abstract: Pregnancy failure caused by early embryonic death in dairy cows can result in significant economic losses to the dairy farm. Early and accurate pregnancy diagnosis in non-pregnant cows will improve the efficiency of pregnancy and shorten the number of days to nulliparity. This paper reviews recent domestic and international studies on pregnancy-associated proteins, body fluid proteins such as blood, urine, and milk of cows in early pregnancy stages, and proteomics to identify potential proteins in early non-pregnancy for accurate pregnancy diagnosis in early pregnancy and to improve dairy production efficiency.

Key words: dairy cows, proteomics, early pregnancy diagnosis, biomarkers, pregnancy-related substances

中图分类号:

S823.91

张馨蕊, 付予, 杨卓, 沈文娟, 陶金忠. 奶牛早期妊娠诊断蛋白的研究[J]. 畜牧兽医学报, 2024, 55(2): 451-460.

ZHANG Xinrui, FU Yu, YANG Zhuo, SHEN Wenjuan, TAO Jinzhong. Study of Early Pregnancy Diagnostic Proteins in Dairy Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 451-460.

参考文献

[1] 冯肖艺,郝海生,杜卫华,等.能量负平衡导致奶牛繁殖力下降的机制进展[J].畜牧兽医学报,2023,54(10):4050-4060.
FENG X Y,HAO H S,DU W H,et al.Progress in mechanism of reduced fertility in dairy cows due to negative energy balance[J].Acta Veterinaria et Zootechnica Sinica,2023,54(10):4050-4060.(in Chinese)
[2] SMITH M F,GEISERT R D,PARRISH J J.Reproduction in domestic ruminants during the past 50 yr:discovery to application[J].J Anim Sci,2018,96(7):2952-2970.
[3] FORDE N,LONERGAN P.Interferon-tau and fertility in ruminants[J].Reproduction,2017,154(5):F33-F43.
[4] SPECKHART S L,OLIVER M A,EALY A D.Developmental hurdles that can compromise pregnancy during the first month of gestation in cattle[J].Animals (Basel),2023,13(11):1760.
[5] 罗芳,张萌,李亚超,等.影响奶牛早期胚胎丢失的因素[J].畜牧兽医学报,2020,51(5):907-913.
LUO F,ZHANG M,LI Y C,et al.Factors associated with early embryo loss in dairy cows[J].Acta Veterinaria et Zootechnica Sinica,2020,51(5):907-913.(in Chinese)
[6] 赵璐.山羊早期胚胎基因组激活过程中高表达lncRNA的筛选和功能研究[D].杨凌:西北农林科技大学,2020.
ZHAO L.Screening and functional study of highly expressed lncRNA during embryonic genome activation in goat[D].Yangling:Northwest A&F University,2020.(in Chinese)
[7] BERG D K,LEDGARD A,DONNISON M,et al.The first week following insemination is the period of major pregnancy failure in pasture-grazed dairy cows[J].J Dairy Sci,2022,105(11):9253-9270.
[8] ADHIKARI B,LEE C N,KHADKA V S,et al.RNA-Sequencing based analysis of bovine endometrium during the maternal recognition of pregnancy[J].BMC Genomics,2022,23(1):494.
[9] GARCIA-ISPIERTO I,LÓPEZ-HELGUERA I,SERRANO-PÉREZ B,et al.Progesterone supplementation during the time of pregnancy recognition after artificial insemination improves conception rates in high-producing dairy cows[J].Theriogenology,2016,85(7):1343-1347.
[10] 魏士昊,戴天姝,王锋,等.奶牛早孕诊断技术研究进展[J].畜牧兽医学报,2022,53(7):2047-2056.
WEI S H,DAI T S,WANG F,et al.Research progress of early pregnancy diagnosis in dairy cows[J].Acta Veterinaria et Zootechnica Sinica,2022,53(7):2047-2056.(in Chinese)
[11] EALY A D,SEEKFORD Z K.Symposium review:predicting pregnancy loss in dairy cattle[J].J Dairy Sci,2019,102(12):11798-11804.
[12] JAŚKOWSKI J M,KACZMAROWSKI M,KULUS J,et al.Rectal palpation for pregnancy checks in cows-the past or an alternative to modern diagnostic methods[J].Med Weter,2019,75(1):6156-2019.
[13] SAMIR H,KANDIEL M M M.Accuracy of subjective evaluation of luteal blood flow by color Doppler ultrasonography for early diagnosis of pregnancy in Egyptian buffalo[J].Anim Reprod Sci,2019,208:106129.
[14] 李福慧.规模化奶牛场B超早期妊娠诊断规程的研究[D].呼和浩特:内蒙古农业大学,2021.
LI F H.Research on Early pregnancy diagnosis procedure by ultrasonography in scaled dairy farm[D].Hohhot:Inner Mongolia Agricultural University,2021.(in Chinese)
[15] FRICKE P M,RICCI A,GIORDANO J O,et al.Methods for and implementation of pregnancy diagnosis in dairy cows[J].Vet Clin North Am Food Anim Pract,2016,32(1):165-180.
[16] 苏妮,孙振师,张化恒.奶牛三种早孕诊断方法的比较[J].山东畜牧兽医,2012,33(4):17-18.
SU N,SUN Z S,ZHANG H H.Comparison of three methods for diagnosing early pregnancy in dairy cows[J].Shandong Journal of Animal Science and Veterinary Medicine,2012,33(4):17-18.(in Chinese)
[17] 李助南,唐登华,郭良辉.血小板计数法对奶牛超早期妊娠的诊断研究[J].黑龙江畜牧兽医,2005(9):15-16.
LI Z N,TANG D H,GUO L H.A diagnostic study of platelet count in ultra-early pregnancy in dairy cows[J].Heilongjiang Animal Science and Veterinary Medicine,2005(9):15-16.(in Chinese)
[18] 罗芳,陶金忠.基于UHPLC-QTOF-MS代谢组学方法筛选奶牛不同妊娠状态时的候选生物标志物[J].畜牧兽医学报,2021,52(11):3118-3125.
LUO F,TAO J Z.Screen of candidate biomarkers for different pregnancy status of cows based on UHPLC-QTOF-MS metabolomics[J].Acta Veterinaria et Zootechnica Sinica,2021,52(11):3118-3125.(in Chinese)
[19] FORDE N,SIMINTIRAS C A,STURMEY R,et al.Amino acids in the uterine luminal fluid reflects the temporal changes in transporter expression in the endometrium and conceptus during early pregnancy in cattle[J].PLoS One,2014,9(6):e100010.
[20] 原开敏,时玉新,董智豪,等.母牛早期妊娠诊断技术研究进展[J].畜牧兽医学报,2023,54(6):2223-2230.
YUAN K M,SHI Y X,DONG Z H,et al.Research progress on early pregnancy diagnosis technologies in cows[J].Acta Veterinaria et Zootechnica Sinica,2023,54(6):2223-2230.(in Chinese)
[21] FAN X G,ZHENG Z Q,FAN X G,et al.A study of early pregnancy factor activity in preimplantation[J].Am J Reprod Immunol,1997,37(5):359-364.
[22] 白云.奶牛四种妊娠诊断方法与结果的比较[D].石河子:石河子大学,2013.
BAI Y.The diagnostic results comparison of four pregnancy diagnostic methods in cows[D].Shihezi:Shihezi University,2013.(in Chinese)
[23] ROMERO J J,ANTONIAZZI A Q,NETT T M,et al.Temporal release,paracrine and endocrine actions of ovine conceptus-derived interferon-tau during early pregnancy[J].Biol Reprod,2015,93(6):146.
[24] GREEN J A,PARKS T E,AVALLE M P,et al.The establishment of an ELISA for the detection of pregnancy-associated glycoproteins (PAGs) in the serum of pregnant cows and heifers[J].Theriogenology, 2005,63(5):1481-1503.
[25] 赵生贤,樊江峰,余四九,等.早孕因子(EPF)在雌性牦牛主要生殖器官中的表达[J].农业生物技术学报,2020,28(5):876-883.
ZHAO S X,FAN J F,YU S J,et al.Expression of early pregnancy factors (EPF) in the main reproductive organs of female yak (Bos grunnien)[J].Journal of Agricultural Biotechnology,2020,28(5):876-883.(in Chinese)
[26] NAHHAS F,BARNEA E.Human embryonic origin early pregnancy factor before and after implantation[J]. Am J Reprod Immunol,1990,22(3-4):105-108.
[27] GROSSO M C,BELLINGERI R V,MOTTA C E,et al.Immunohistochemical distribution of early pregnancy factor in ovary,oviduct and placenta of pregnant gilts[J].Biotech Histochem,2015,90(1):14-24.
[28] OTT T L.Immunological detection of pregnancy:evidence for systemic immune modulation during early pregnancy in ruminants[J].Theriogenology,2020,150:498-503.
[29] 李雯乔,李发弟,王新基,等.家畜早期妊娠诊断的研究进展[J].畜牧兽医学报,2023,54(5):1782-1791.
LI W Q,LI F D,WANG X J,et al.Advance of early pregnancy diagnosis in livestock[J].Acta Veterinaria et Zootechnica Sinica,2023,54(5):1782-1791.(in Chinese)
[30] LALEH V G,LALEH R G,PIRANY N,et al.Measurement of EPF for detection of cow pregnancy using rosette inhibition test[J].Theriogenology,2008,70(1):105-107.
[31] 张宏刚,陈树林,王晓珊,等.孕牛血清早孕因子的生物活性检测及妊娠率分析[J].动物医学进展,2010,31(8):30-33.
ZHANG H G,CHEN S L,WANG X S,et al.Detection of biological activity of EPF from sera of pregnant cows and analysis of the pregnant rate[J].Progress in Veterinary Medicine,2010,31(8):30-33.(in Chinese)
[32] 刘水喜.奶牛早期妊娠诊断几种方法的比较[J].畜禽业,2016,27(12):35-36.
LIU S X.Comparison of several methods for early pregnancy diagnosis in dairy cows[J].Livestock and Poultry Industry,2016,27(12):35-36.(in Chinese)
[33] HAN X,WANG S Y,REN Y,et al.Selection of early pregnancy specific proteins and development a rapid immunochromatographic test strip in cows[J].Theriogenology,2022,187:127-134.
[34] LIU J F,WU Z M,GUO S,et al.IFN-τ attenuates LPS-induced endometritis by restraining HMGB1/NF-κB activation in bEECs[J].Inflammation,2021,44(4):1478-1489.
[35] HIRAYAMA H,MORIYASU S,KAGEYAMA S,et al.Enhancement of maternal recognition of pregnancy with parthenogenetic embryos in bovine embryo transfer[J].Theriogenology,2014,81(8):1108-1115.
[36] TOJI N,SHIGENO S,KIZAKI K,et al.Evaluation of interferon-stimulated genes in peripheral blood granulocytes as sensitive responders to bovine early conceptus signals[J].Vet J,2017,229:37-44.
[37] SUN M,HAO F,LI W L,et al.Temporal dynamics of the ruminant type I IFN-induced antiviral state against homologous parainfluenza virus 3 challenge in vitro[J].Viruses,2022,14(5):1025.
[38] HARA K,SHIRASUNA K,USUI F,et al.Interferon-tau attenuates uptake of nanoparticles and secretion of interleukin-1β in macrophages[J].PLoS One,2014,9(12):e113974.
[39] 朱喆.干扰素-τ调节妊娠早期奶牛子宫内膜BoLA-Ⅰ机制研究[D].武汉:华中农业大学,2017.
ZHU Z.The mechanism of interferon-τ regulate endometrial BoLA-Ⅰ during early pregnancy in dairy cows[D].Wuhan:Huazhong Agricultural University,2017.(in Chinese)
[40] 程蕾,王定发,刘晓华,等.ISG15和OAS1在奶牛早期妊娠阶段外周血中的表达规律[J].畜牧兽医学报,2015,46(1):77-84.
CHENG L,WANG D F,LIU X H,et al.Study on ISG15 and OAS1 transcriptions in peripheral blood of dairy cows during early pregnancy[J].Acta Veterinaria et Zootechnica Sinica,2015,46(1):77-84.(in Chinese)
[41] CASANO A B,BARILE V L,MENCHETTI L,et al.Interferon tau (IFNt) and interferon-stimulated genes (ISGs) expression in peripheral blood leukocytes and correlation with circulating pregnancy-associated glycoproteins (PAGs) during peri-implantation and early pregnancy in buffalo cows[J].Animals (Basel), 2022, 12(22):3068.
[42] PUGLIESI G,MIAGAWA B T,PAIVA Y N,et al.Conceptus-induced changes in the gene expression of blood immune cells and the ultrasound-accessed luteal function in beef cattle:how early can we detect pregnancy?[J].Biol Reprod,2014,91(4):95.
[43] DE MELO G D,MELLO B P,FERREIRA C A,et al.Applied use of interferon-tau stimulated genes expression in polymorphonuclear cells to detect pregnancy compared to other early predictors in beef cattle[J]. Theriogenology, 2020,152:94-105.
[44] YOSHINO H,TOJI N,SASAKI K,et al.A predictive threshold value for the diagnosis of early pregnancy in cows using interferon-stimulated genes in granulocytes[J].Theriogenology,2018,107:188-193.
[45] POHLER K G,REESE S T,FRANCO G A,et al.New approaches to diagnose and target reproductive failure in cattle[J].Anim Reprod,2020,17(3):e20200057.
[46] 骆金红,陈祥,尚以顺,等.转录组测序筛选山羊妊娠早期胚胎附植相关基因[J].畜牧兽医学报,2022,53(5):1465-1474.
LUO J H,CHEN X,SHANG Y S,et al.Transcriptome sequencing screening the genes related to goat embryo attachment in early pregnancy[J].Acta Veterinaria et Zootechnica Sinica,2022,53(5):1465-1474.(in Chinese)
[47] GIORDANO J O,GUENTHER J N,LOPES G,et al.Changes in serum pregnancy-associated glycoprotein,pregnancy-specific protein B,and progesterone concentrations before and after induction of pregnancy loss in lactating dairy cows[J].J Dairy Sci,2012,95(2):683-697.
[48] REESE S T,PEREIRA M H C,EDWARDS J L,et al.Pregnancy diagnosis in cattle using pregnancy associated glycoprotein concentration in circulation at day 24 of gestation[J].Theriogenology,2018,106:178-185.
[49] 钟旭,薛立群,田见晖.妊娠相关糖蛋白(PAG)在奶牛早期妊娠诊断上的应用现状[J].中国畜牧兽医,2011,38(11):140-143.
ZHONG X,XUE L Q,TIAN J H.Early pregnancy diagnosis by pregnancy associated glycoprotein in dairy cattle[J].China Animal Husbandry & Veterinary Medicine,2011,38(11):140-143.(in Chinese)
[50] FRIEDRICH M,HOLTZ W.Establishment of an ELISA for measuring bovine pregnancy-associated glycoprotein in serum or milk and its application for early pregnancy detection[J].Reprod Domest Anim,2010,45(1):142-146.
[51] DUROCHER J,MOORE R K,CASTONGUAY M H,et al.Bayesian estimation of sensitivity and specificity of a milk pregnancy-associated glycoprotein ELISA test for pregnancy diagnosis between 23 and 27 days after insemination in Holstein dairy cows[J].J Dairy Sci,2022,105(8):6985-6996.
[52] POHLER K G,PEREIRA M H C,LOPES F R,et al.Circulating concentrations of bovine pregnancy-associated glycoproteins and late embryonic mortality in lactating dairy herds[J].J Dairy Sci,2016,99(2):1584-1594.
[53] FRISÉE V,RIGAUX G,DUFOUR P,et al.American Bison (Bison bison) reproductive endocrinology:serum Pregnancy Associated Glycoproteins (PAG),Progesterone,Estrone and Estrone-Sulfate in non pregnant animals and during gestation[J].Domest Anim Endocrinol,2022,78:106684.
[54] VELEK K,MICHAUD S,BOUCHER K,等.爱德士牛怀孕检测[C]//中国奶业协会第26次繁殖学术年会暨国家肉牛牦牛/奶牛产业技术体系第3届全国牛病防治学术研讨会论文集.兰州,2011:140-143.
VELEK K,MICHAUD S,BOUCHER K,et al.IDEXX cattle pregnancy test[C]//Proceedings of the 26th Annual Conference of Breeding of China Dairy Association and the 3rd National Symposium on Cattle Disease Prevention and Control of National Beef Cattle,Yak/Dairy Cattle Industry Technology System.Lanzhou,2011:140-143.(in Chinese)
[55] BAHUGUNA C,SHARMA M.Proteomic analysis of serum protein during early pregnancy[J].J Proteins Proteomics,2022,13(1):1-15.
[56] PANDA B S K,MOHAPATRA S K,CHAUDHARY D,et al.Proteomics and transcriptomics study reveals the utility of ISGs as novel molecules for early pregnancy diagnosis in dairy cows[J].J Reprod Immunol,2020, 140:103148.
[57] JOHNSTON D,MALO ESTEPA I,EBHARDT H A,et al.Differences in the bovine milk whey proteome between early pregnancy and the estrous cycle[J].Theriogenology,2018,114:301-307.
[58] RAWAT P,BATHLA S,BAITHALU R,et al.Identification of potential protein biomarkers for early detection of pregnancy in cow urine using 2D DIGE and label free quantitation[J].Clin Proteomics,2016,13:15.
[59] ARANCIAGA N,MORTON J D,MAES E,et al.Proteomic determinants of uterine receptivity for pregnancy in early and mid-postpartum dairy cows[J].Biol Reprod,2021,105(6):1458-1473.
[60] MORAES J G N,BEHURA S K,BISHOP J V,et al.Analysis of the uterine lumen in fertility-classified heifers:II.Proteins and metabolites[J].Biol Reprod,2020,102(3):571-587.
[61] KIZAKI K,SHICHIJO-KIZAKI A,FURUSAWA T,et al.Differential neutrophil gene expression in early bovine pregnancy[J].Reprod Biol Endocrinol,2013,11:6.
[62] 余婕,向敏,刘辰晖,等.干扰素-τ调控牛母体妊娠识别分子机制的研究进展[J].中国奶牛,2021(8):28-32.
YU J,XIANG M,LIU C H,et al.Research progress on molecular mechanism of maternal recognition of pregnancy regulated by interferon-tau in cows[J].China Dairy Cattle,2021(8):28-32.(in Chinese)
[63] SZELÉNYI Z,SZENCI O,BODÓ S,et al.Noninfectious causes of pregnancy loss at the late embryonic/early fetal stage in dairy cattle[J].Animals (Basel),2023,13(21):3390.
[64] HAN R X,KIM H R,DIAO Y F,et al.Detection of early pregnancy-specific proteins in Holstein milk[J].J Proteomics,2012,75(11):3221-3229.
[65] KUMAR R,ALI S A,SINGH S K,et al.Peptide profiling in cow urine reveals molecular signature of physiology-driven pathways and in-silico predicted bioactive properties[J].Sci Rep,2021,11(1):12427.
[66] SPENCER T E,FORDE N,LONERGAN P.Insights into conceptus elongation and establishment of pregnancy in ruminants[J].Reprod Fertil Dev,2017,29(1):84-100.
[67] LAMY J,LABAS V,HARICHAUX G,et al.Regulation[LL]of the bovine oviductal fluid proteome[J]. Reproduction, 2016,152(6):629-644.
[68] MYERS T D,PALLADINO M J.Newly discovered roles of triosephosphate isomerase including functions within the nucleus[J].Mol Med,2023,29(1):18.
[69] BELTMAN M E,MULLEN M P,ELIA G,et al.Global proteomic characterization of uterine histotroph recovered from beef heifers yielding good quality and degenerate day 7 embryos[J].Domest Anim Endocrinol, 2014, 46:49-57.
[70] ARTUS J,HUE I,ACLOQUE H.Preimplantation development in ungulates:a 'ménage à quatre' scenario[J]. Reproduction,2020,159(3):R151-R172.
[71] 张航,杨柏高,徐茜,等.热应激影响奶牛胚胎发育作用机制的研究进展[J].畜牧兽医学报,2023, 54(7):2692-2700.
ZHANG H,YANG B G,XU X,et al.Research progress on the mechanism of heat stress affecting the development of dairy cow embryos[J].Acta Veterinaria et Zootechnica Sinica,2023,54(7):2692-2700.(in Chinese)
[72] MUÑOZ M,CORRALES F J,CAAMANÑO J N,et al.Proteome of the early embryo-maternal dialogue in the cattle uterus[J].J Proteome Res,2012,11(2):751-766.