营养转运相关基因调控区多态性与黄羽肉鸡饲料转化率关联分析

doi:10.11843/j.issn.0366-6964.2023.06.014

摘要/Abstract

摘要： 旨在探讨多个营养转运相关基因调控区的多态性,并筛选影响肉鸡饲料转化率的关键变异位点。本研究采集了439只雄性黄羽肉鸡的血液DNA样本,利用PCR直接测序的方法在鸡群高、低饲料转化率的两尾样本组中(高FCR组>3.40,低FCR组<2.35,样本数均为12个)对3个单糖转运基因(GLUT2、GLUT5和SGLT1)、8个氨基酸转运基因(B⁰AT1、B⁰⁺AT、CAT1、CAT2、LAT1、y⁺LAT1、y⁺LAT2和rBAT)和1个营养调控基因(FGF1)上游1 kb内的多态性位点进行检测。依据两尾样本组中基因型频率的差异,筛选潜在影响饲料转化率的变异位点。采用PCR-RFLP技术在全群样本中对变异位点进行基因分型,并分析这些多态性位点与饲料转化率等性状的关联性。结果显示,本研究在12个基因的上游1 kb范围内共寻找到111个SNPs位点,其中有8个SNPs位点在两尾样本组中的基因型频率存在差异,分别为GLUT2基因的-379A>G,SGLT1基因的-490G>A,B⁰AT1基因的-639T>C,rBAT基因的-46G>A、-90G>T、-180T>C、-235G>A和FGF1基因的-884T>C位点。在全群样本中对GLUT2基因的-379A>G、B⁰AT1基因的-639T>C和FGF1基因的-884T>C位点的基因分型和关联分析发现,B⁰AT1基因的-639T>C位点与饲料转化率存在显著相关(P<0.05),TC基因型个体的饲料转化率显著低于TT和CC基因型个体(P<0.05),表现出杂合子优势。FGF1基因的-884T>C位点也与饲料转化率显著相关(P<0.05),CC和TT基因型个体的饲料转化率显著低于TC基因型个体(P<0.05),此外,GLUT2基因的-379A>G与饲料转化率或生长性状均无显著相关。本研究分析了多个营养转运相关基因调控区的遗传变异,为将这些变异位点作为遗传标记用于提高黄羽肉鸡饲料转化率的分子选育提供了依据。

关键词: 黄羽肉鸡, 饲料转化率, 关联分析, 营养转运

Abstract: The study aimed to investigate the polymorphism of regulatory regions of several nutrition transport-related genes and screen the key variations affecting the feed conversion ratio of broilers. The blood DNA samples of 439 male yellow-feather broilers were collected in this study, and the polymorphic loci within 1 kb upstream of 3 monosaccharide transporter genes (GLUT2, GLUT5 and SGLT1), 8 amino acid transporter genes (B⁰AT1, B⁰⁺AT, CAT1, CAT2, LAT1, y⁺LAT1, y⁺LAT2 and rBAT) and 1 nutrient regulation gene (FGF1) were detected in the two-tail sample group with high and low feed conversion ratio by PCR sequencing. According to the difference in genotype frequency in the two-tail sample groups, the genetic variations with potential influence on feed conversion ratio were screened. The PCR-RFLP method was used to genotype the polymorphic loci in the whole sample and the association between these polymorphic loci with feed conversion ratio and other traits was analyzed. The results showed that a total of 111 SNPs sites were found within 1 kb upstream of 12 genes in this study, among which 8 SNPs sites had different genotype frequencies in the two-tail sample groups,including -379A>G of GLUT2, -490G>A of SGLT1, -639T>C of B⁰AT1, -46G>A, -90G>T, -180T>C, -235G>A of rBAT, and -884T>C of FGF1. Further whole population genotyping and association analysis showed that the -639T>C of B⁰AT1 was significantly associated with feed conversion ratio (P<0.05), individuals with TC genotype had significantly lower feed conversion ratio than those with TT and CC genotypes (P<0.05), showed a heterozygous advantage. The -884T>C of FGF1 was also significantly associated with feed conversion ratio (P<0.05), and the individuals with CC and TT genotypes had significantly lower feed conversion ratio than those with the TC genotype (P<0.05). In addition, the -379A>G of GLUT2 was not significantly associated with feed conversion or growth traits. In this study, the genetic variation of regulatory regions of several nutrient transport-related genes was analyzed, which provides the basis for using these variation sites as genetic markers to improve the feed conversion ratio of yellow-feather broilers.

Key words: yellow-feather boiler, feed conversion ratio, association study, nutrient transport

中图分类号:

S831.2

王政, 郭文婕, 程瑾, 原一桐, 罗榕, 薛毅, 张利环, 朱芷葳, 李慧锋. 营养转运相关基因调控区多态性与黄羽肉鸡饲料转化率关联分析[J]. 畜牧兽医学报, 2023, 54(6): 2343-2352.

WANG Zheng, GUO Wenjie, CHENG Jin, YUAN Yitong, LUO Rong, XUE Yi, ZHANG Lihuan, ZHU Zhiwei, LI Huifeng. Association Study of Polymorphism in the Regulatory Region of Nutrient Transport-related Genes and Feed Conversion Ratio in Yellow-feather Broiler[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2343-2352.

参考文献 18

[1]	李森,杜永旺,文杰,等.快速型黄羽肉鸡饲料利用效率性状的基因组选择研究[J].畜牧兽医学报,2021,52(8):2151-2161.LI S,DU Y W,WEN J,et al.A study of genomic selection for feed efficiency traits in fast-growing yellow-feathered broilers[J].Acta Veterinaria et Zootechnica Sinica,2021,52(8):2151-2161.(in Chinese)
[2]	SELIM S,ABDEL-MEGEID N S,KHALIFA H K,et al.Efficacy of various feed additives on performance,nutrient digestibility,bone quality,blood constituents,and phosphorus absorption and utilization of broiler chickens fed low phosphorus diet[J].Animals (Basel),2022,12(14):1742.
[3]	SUNDARAM S,BORTHAKUR A.Altered intestinal epithelial nutrient transport:an underappreciated factor in obesity modulated by diet and microbiota[J].Biochem J,2021,478(5):975-995.
[4]	KOEPSELL H.Glucose transporters in the small intestine in health and disease[J].Pflugers Arch,2020,472(9):1207-1248.
[5]	张利环,张若男,贾浩,等.益生菌互作对肉鸡生长性能、肠道消化吸收及糖转运蛋白GLUT2影响的研究[J].畜牧兽医学报, 2020,51(9):2165-2176.ZHANG L H,ZHANG R N,JIA H,et al.Effects of Probiotics interaction on growth performance,intestinal digestion and absorption,and sugar transporter GLUT2 in broilers[J].Acta Veterinaria et Zootechnica Sinica,2020,51(9):2165-2176.(in Chinese)
[6]	LIANG R J,TAYLOR S,NAHIYAAN N,et al.GLUT5(SLC2A5) enables fructose-mediated proliferation independent of ketohexokinase[J].Cancer Metab,2021,9(1):12.
[7]	BESKI S S M,SWICK R A,IJI P A.Specialized protein products in broiler chicken nutrition:a review[J].Anim Nutr, 2015, 1(2):47-53.
[8]	LIU L J,ZHANG S Y,BAO J Y,et al.Melatonin improves laying performance by enhancing intestinal amino acids transport in hens[J].Front Endocrinol (Lausanne),2018,9:426.
[9]	陈赛娟,刘亚娟,袁万哲,等.家兔小肠不同区段营养物质转运载体相关基因的分布规律[J].畜牧兽医学报, 2019,50(7):1518-1524.CHEN S J,LIU Y J,YUAN W Z,et al.Distribution of nutrient transporter related genes in different segments of small intestine of rabbits[J].Acta Veterinaria et Zootechnica Sinica,2019,50(7):1518-1524.(in Chinese)
[10]	JAVED K,FAIRWEATHER S J.Amino acid transporters in the regulation of insulin secretion and signalling[J].Biochem Soc Trans,2019,47(2):571-590.
[11]	KLETA R,ROMEO E,RISTIC Z,et al.Mutations in SLC6A19,encoding B0AT1,cause Hartnup disorder[J].Nat Genet,2004, 36(9):999-1002.
[12]	SANCAR G,LIU S H,GASSER E,et al.FGF1 and insulin control lipolysis by convergent pathways[J].Cell Metab,2022, 34(1): 171-183.e6.
[13]	SUH J M,JONKER J W,AHMADIAN M,et al.Endocrinization of FGF1 produces a neomorphic and potent insulin sensitizer[J]. Nature,2014,513(7518):436-439.
[14]	MARCHESI J A O,ONO R K,CANTÃO M E,et al.Exploring the genetic architecture of feed efficiency traits in chickens[J].Sci Rep,2021,11(1):4622.
[15]	TRELEAVEN T,ZADA M,NAGARAJAH R,et al.Stage-specific L-proline uptake by amino acid transporter Slc6a19/B0AT1 is required for optimal preimplantation embryo development in mice[J].Cells,2022,12(1):18.
[16]	BRÖER S.The role of the neutral amino acid transporter B0AT1(SLC6A19) in Hartnup disorder and protein nutrition[J]. IUBMB Life,2009,61(6):591-599.
[17]	JAVED K,CHENG Q,CARROLL A J,et al.Development of biomarkers for inhibition of SLC6A19(B0AT1)—a potential target to treat metabolic disorders[J].Int J Mol Sci,2018,19(11):3597.
[18]	YANG H S,FU D Z,SHAO H,et al.Impacts of birth weight on plasma,liver and skeletal muscle neutral amino acid profiles and intestinal amino acid transporters in suckling Huanjiang mini-piglets[J].PLoS One,2012,7(12):e50921.