华西牛胴体及原始分割肉块重量性状遗传参数估计与全基因组关联分析

doi:10.11843/j.issn.0366-6964.2023.09.008

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (9): 3664-3676.doi: 10.11843/j.issn.0366-6964.2023.09.008

华西牛胴体及原始分割肉块重量性状遗传参数估计与全基因组关联分析

李柯安宁¹, 杜丽丽¹, 安炳星¹, 邓天宇^1,2, 梁忙¹, 曹晟^1,3, 杜悦莹^1,4, 徐凌洋¹, 高雪¹, 张路培¹, 李俊雅¹, 高会江^1*

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 西北农林科技大学动物科技学院, 杨凌 712100;
3. 天津农学院计算机与信息工程学院, 天津 300384;
4. 青岛农业大学动物科技学院, 青岛 266109

收稿日期:2022-10-31 发布日期:2023-09-22
通讯作者: 高会江,主要从事动物遗传育种与繁殖研究,E-mail:gaohuijiang@caas.cn
作者简介:李柯安宁(1998-),男,浙江玉环人,硕士生,主要从事动物遗传育种与繁殖研究,E-mail:likeanning@163.com
基金资助:
国家自然科学基金(31872975);内蒙古自治区科学技术计划(2020GG0210);国家肉牛牦牛产业技术体系(CARS-37);中央级公益性科研院所基本科研业务费专项资金项目(2022-YWF-ZX-04);财政部和农业农村部:国家现代农业产业技术体系资助

Genetic Parameter Estimation and Genome-Wide Association Study for Carcass Traits and Primal Cuts Weight Traits in Huaxi Cattle

LI Keanning¹, DU Lili¹, AN Bingxing¹, DENG Tianyu^1,2, LIANG Mang¹, CAO Sheng^1,3, DU Yueying^1,4, XU Lingyang¹, GAO Xue¹, ZHANG Lupei¹, LI Junya¹, GAO Huijiang^1*

1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China;
3. College of Computer and Information Engineering, Tianjin Agricultural University, Tianjin 300384, China;
4. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China

Received:2022-10-31 Published:2023-09-22

摘要/Abstract

摘要： 旨在利用基因组信息来估计华西牛胴体性状与原始分割肉块重量性状遗传参数并挖掘与之相关的重要候选基因。本研究以1 585头18月龄华西牛屠宰个体17个胴体及原始分割肉块重量性状为研究对象,利用GCTA与R软件以及770K高密度芯片数据,对17个性状进行遗传参数估计以及候选基因挖掘。结果显示,四肢及臀部区域原始分割肉块重量性状属于高遗传力性状(0.41~0.57),躯干部分原始分割肉块重量以及出栏重、胴体重、屠宰率、净肉率属于中等遗传力性状(0.19~0.39)。GWAS分析共检测出26个显著SNPs位点并定位到24个候选基因,富集分析显示相关候选基因参与细胞增殖、脂质代谢及能量转换等过程,NSMF、NOXA、TEFM、MATN1、MKX、FOXO1等基因为华西牛肌肉生长候选基因。结果为华西牛后续育种策略的细化提供参考。

关键词: 华西牛, 胴体性状, 原始分割肉块重量性状, 遗传参数, 全基因组关联分析

Abstract: This study aimed to estimate the genetic parameters for carcass and primal cuts weight traits and excavate the related candidate genes in Huaxi cattle by using genomic information. In this study, 17 carcass traits and primal cuts weight traits from 1 585 18-months-old slaughter Huaxi cattle were selected as the research subjects, the R program, GCTA software and 770K high density chip data were utilized to estimate the genetic parameters and excavate the related candidate genes for these 17 traits. The results showed that the primal cuts weight in extremities and hip area were high heritability traits (0.41-0.57), while the primal cuts weight in trunk, marketing weight, carcass weight, dressing percentage and meat percentage were medium heritability traits (0.19-0.39). Ultimately, 26 significant SNPs were detected by genome-wide association study and 24 candidate genes were located. Enrichment analysis showed these candidate genes were associated with the cell proliferation, lipid metabolism, cellular energy conversion, etc. NSMF, NOXA, TEFM, MATN1, MKX, FOXO1 were the promising candidate genes for muscle growth in Huaxi cattle. These results might provide reference for the optimization of the subsequent breeding program of Huaxi cattle.

Key words: Huaxi cattle, carcass trait, primal cuts weight trait, genetic parameter, genome-wide association study

中图分类号:

S823.2

李柯安宁, 杜丽丽, 安炳星, 邓天宇, 梁忙, 曹晟, 杜悦莹, 徐凌洋, 高雪, 张路培, 李俊雅, 高会江. 华西牛胴体及原始分割肉块重量性状遗传参数估计与全基因组关联分析[J]. 畜牧兽医学报, 2023, 54(9): 3664-3676.

LI Keanning, DU Lili, AN Bingxing, DENG Tianyu, LIANG Mang, CAO Sheng, DU Yueying, XU Lingyang, GAO Xue, ZHANG Lupei, LI Junya, GAO Huijiang. Genetic Parameter Estimation and Genome-Wide Association Study for Carcass Traits and Primal Cuts Weight Traits in Huaxi Cattle[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3664-3676.

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华西牛胴体及原始分割肉块重量性状遗传参数估计与全基因组关联分析

Genetic Parameter Estimation and Genome-Wide Association Study for Carcass Traits and Primal Cuts Weight Traits in Huaxi Cattle

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