Abstract:

The aim of this study was to provide reference for genetic evolution, antigenic specificity and pathogenicity of goose parvovirus (GPV) in Anhui Province of China. The complete genomic sequences of two GPV strains isolated from Muscovy ducks (GPV Y stain) and geese (GPV E stain) in Anhui Province were obtained by polymerase chain reaction method and then aligned with the sequences of GPV and Muscovy duck parvovirus (MDPV) published in GenBank using the neighbor-joining method. The results showed that the genome of the GPV Y stain (5 106 bp) was shorter than that of the GPV E stain (5 125 bp), which was attributed to a 1-bp deletion in the ITR region and a 22-bp addition in the region (4646-4668 nt) between the end of VP3 ORF and the beginning of its poly(A) tail in the GPV E stain. The phylogenetic analyses revealed that these two strains along with those from Taiwan Province of China belonged to the GPV subgroup IIa, while most of other GPV stains isolated in mainland China were clustered in the GPV subgroup IIb. This result further confirmed the high homology of the two isolates to those from Taiwan Province. Compared with the standard virulent GPV B strain, the two isolates missed the deduced 703-705NRT glycosylation site in VP region, which may affect the viral virulence and even cause immune escape.