猪流产胎儿中猪圆环病毒3型的检测及其遗传演化分析

doi:10.11843/j.issn.0366-6964.2022.05.020

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (5): 1527-1535.doi: 10.11843/j.issn.0366-6964.2022.05.020

猪流产胎儿中猪圆环病毒3型的检测及其遗传演化分析

何庆¹, 李周勉¹, 王欢², 李丹彤¹, 谭庆辉³, 邹亚文¹, 苏建明¹, 邓治邦¹, 杨毅¹, 王乃东^1*

1. 湖南农业大学动物医学院兽用蛋白质工程疫苗湖南省重点实验室兽用疫苗逆向创制湖南省工程研究中心, 长沙 410128;
2. 长沙市望城区高塘岭街道农业农村综合服务中心, 长沙 410128;
3. 娄底市动物疫病预防控制中心, 娄底 417000

收稿日期:2021-09-09 出版日期:2022-05-23 发布日期:2022-05-25
通讯作者: 王乃东,主要从事兽医临床微生物组学与生物技术研究,E-mail:naidongwang@hunau.edu.cn
作者简介:何庆(1997-),男,湖南永州人,硕士生,主要从事临床兽医微生物组学与生物技术研究,E-mail:qinghe@stu.hunau.edu.cn;李周勉(1995-),湖南长沙人,硕士生,主要从事临床兽医微生物组学与生物技术研究,E-mail:290249925@qq.com。
基金资助:
国家自然科学基金(32172844)；湖南省自然科学基金面上项目(2018JJ2177)；湖南农业大学“双一流”建设项目(SYL2019048)

Detection and Genetic Evolution of Porcine Circovirus Type 3 in Swine Aborted Fetus

HE Qing¹, LI Zhoumian¹, WANG Huan², LI Dantong¹, TAN Qinghui³, ZOU Yawen¹, SU Jianming¹, DENG Zhibang¹, YANG Yi¹, WANG Naidong^1*

1. College of Veterinary Medicine, Hunan Agricultural University, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, R & D Center for Animal Reverse Vaccinology of Hunan Province, Changsha 410128, China;
2. Gaotangling Street Agricultural and Rural Comprehensive Service Center in Wangcheng District, Changsha 410128, China;
3. Animal Disease Prevention and Control Center, Loudi 417000, China

Received:2021-09-09 Online:2022-05-23 Published:2022-05-25

摘要/Abstract

摘要： 旨在分析猪流产胎儿中猪圆环病毒3型(porcine circovirus type 3，PCV3)的感染及其遗传进化情况。对2015—2017年采集的湖南省680份猪流产胎儿病料样品进行PCV3检测、扩增和测序，并利用生物信息学软件对获得的序列进行遗传进化分析。结果显示，猪流产胎儿样品中PCV3阳性率为24.4%(166/680)，其中，2015—2017年阳性率分别为18.2%(26/143)、22.7%(54/238)和28.8%(86/299)。从PCV3阳性样本中获得了1株PCV3基因组全长序列，命名为PCV3/CN/Hunan-57(GenBank序列登录号为MZ934695)，与国内外毒株基因组序列相比，相似性为97.8%~99.2%；获得了5株PCV3 ORF2基因序列，它们的核苷酸和氨基酸相似性为97.5%~98.3%和96.7%~99.7%，与国内外参考毒株ORF2基因序列相比，其核苷酸和氨基酸相似性为96.3%~99.2%和96.8%~100%。基于ORF2基因序列构建遗传进化树和进行多重序列比对，发现获得的PCV3阳性序列分别属于PCV3a(1株)和PCV3c(4株)，并鉴定出T100A、G113S和A184S突变位点。综上表明，猪流产胎儿中PCV3感染率较高，且存在不同类型的PCV3毒株感染，为后续深入研究PCV3遗传变异及其相关致病性提供了重要参考信息。

关键词: 猪圆环病毒3型, 全基因组, Cap, 遗传演化分析

Abstract: The study aimed to explore the prevalence and genetic evolution of porcine circovirus type 3 (PCV3) in aborted fetuses. In this study, PCV3 detection, amplification and sequencing were performed on 680 samples of porcine abortion fetus in Hunan Province from 2015 to 2017, and the obtained sequences were analyzed by bioinformatics software for genetic evolution. The results showed that the positive rate of PCV3 in fetal samples of porcine abortion was 24.4% (166/680), and the positive rates from 2015 to 2017 were 18.2% (26/143), 22.7% (54/238) and 28.8% (86/299), respectively. A full-length genomic sequence of PCV3 was obtained from PCV3 positive samples, which was named PCV3/CN/Hunan-57 (GenBank accession No. MZ934695). Compared with the genome sequences of PCV3 strains at home and abroad, the homology was 97.8%-99.2%. The ORF2 gene sequences of five PCV3 strains were obtained, and their nucleotide and amino acid homology were 97.5%-98.3% and 96.7%-99.7%, respectively. Compared with the ORF2 gene sequences of reference strains in China and abroad, their nucleotide and amino acid homology were 96.3%-99.2% and 96.8%-100.0%, respectively. The genetic evolution tree was constructed based on the ORF2 gene sequence and multiple sequence alignment was performed. The obtained positive sequence belonged to PCV3a (1 strain) and PCV3c (4 strains), respectively, and the mutation sites of T100A, G113S and A184S were identified. This study showed that the infection rate of PCV3 in porcine aborted fetuses was high. There were different types of PCV3 infection in porcine aborted fetuses, which provided important reference information for further study of PCV3 genetic variation and its pathogenicity.

Key words: porcine circovirus type 3, genome, Cap, genetic evolution

中图分类号:

S852.659.2

何庆, 李周勉, 王欢, 李丹彤, 谭庆辉, 邹亚文, 苏建明, 邓治邦, 杨毅, 王乃东. 猪流产胎儿中猪圆环病毒3型的检测及其遗传演化分析[J]. 畜牧兽医学报, 2022, 53(5): 1527-1535.

HE Qing, LI Zhoumian, WANG Huan, LI Dantong, TAN Qinghui, ZOU Yawen, SU Jianming, DENG Zhibang, YANG Yi, WANG Naidong. Detection and Genetic Evolution of Porcine Circovirus Type 3 in Swine Aborted Fetus[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1527-1535.

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猪流产胎儿中猪圆环病毒3型的检测及其遗传演化分析

Detection and Genetic Evolution of Porcine Circovirus Type 3 in Swine Aborted Fetus

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