Abstract:

The objective of this study is to investigate the relationship between pathological lesion and transcription, expression and distribution of heat shock protein 60 (HSP60) in kidney of chickens infected by Marek's disease virus (MDV). Tumor animal model was successfully established by infecting 1 day old chickens with MDV. Real-time RT-PCR, histopathological, immunohistochemistrical methods were used to detect the pathological lesion, transcription level, expression level and distribution of HSP60 in the kidney. After 21 days post-infection (PI), obvious pathological damage appeared in the kidneys of chickens infected by MDV. HSP60 was mainly distributed in the cytoplasm of the oncocyte and interstitial macrophages in the tumor regions. Within the course of MD, the contents of HSP60 of effected group were always higher than blank control group and vaccine control group, which was significantly higher after 28 days, and the mostly was about 8.608 and 12.752 times at the age of 28 days. The tendency changes looked like a downward parabola, during 7 to 21-day-old, the level of HSP60 mRNA transcription of effected group was in the rising stage, and then gradually dropped. When 21-day-old the maximum was came on, which was about 1.222 and 1.179 times of two control groups. Throughout all stages the level of transcription was higher than blank control group, and during 14 to 28-day-old was significantly higher (P<0.01). The MDV infection caused resistance to infection and antitumor response in the progress of MD, the stress related kidney damage resulted in the over-expression of HSP60 in tumor lesions and surrounding tissues. A corresponding increase came to the mRNA transcription level of it in kidney tissues. The rapid expression changes of HSP60 was basic consistent with its mRNA transportation level, but not completely linear. And they were closely related with the onset and progression of tumors, which might be hallmarks to diagnose and determine the process of tumor caused by MDV.