猪圆环病毒3型的分离及致病性分析

doi:10.11843/j.issn.0366-6964.2023.04.020

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (4): 1568-1578.doi: 10.11843/j.issn.0366-6964.2023.04.020

猪圆环病毒3型的分离及致病性分析

库旭钢^1,3,4, 于学祥^1,2,3, 孙琪^1,2,3, 李盼盼^1,3, 张梦佳^1,2, 罗锐^1,2, 钱平^1,2*, 何启盖^1,2,3,4*

1. 华中农业大学动物医学院, 武汉 430070;
2. 农业微生物学国家重点实验室, 武汉 430070;
3. 生猪健康养殖协同创新中心, 武汉 430070);
4. 华中农业大学动物疫病诊断中心, 武汉 430070

收稿日期:2022-04-26 出版日期:2023-04-23 发布日期:2023-04-27
通讯作者: 何启盖,主要从事猪传染病防控与净化研究,E-mail:he628@mail.hzau.edu.cn
作者简介:库旭钢(1984-),男,河南许昌人,博士,主要从事动物传染病研究,E-mail:kuxugang84@163.com
基金资助:
财政部和农业农村部：国家生猪产业技术体系（CARS-35）

Isolation and Pathogenicity Analysis of Porcine Circovirus Type 3

KU Xugang^1,3,4, YU Xuexiang^1,2,3, SUN Qi^1,2,3, LI Panpan^1,3, ZHANG Mengjia^1,2, LUO Rui^1,2, QIAN Ping^1,2*, HE Qigai^1,2,3,4*

1. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China;
2. State Key Laboratory of Agricultural Microbiology, Wuhan 430070, China;
3. The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China;
4. Animal Disease Diagnosis Center, Huazhong Agricultural University, Wuhan 430070, China

Received:2022-04-26 Online:2023-04-23 Published:2023-04-27

摘要/Abstract

摘要： 猪圆环病毒3型（porcine circovirus type 3，PCV3）是2016年由美国首次报道引起母猪繁殖障碍的一种新型猪圆环病毒，该病毒的致病性尚不完全清楚。为研究PCV3对断奶仔猪的致病性，本研究用PK-15细胞自临床病料分离到1株PCV3毒株，命名为PCV3/CH/HB/XY/2018，全基因组测序分析表明，该毒株属于PCV3a-IM基因亚型。分别用PCV3阳性病料初始匀浆液和第8代细胞培养物采用滴鼻和肌肉注射2种方式感染28日龄断奶仔猪，每组试验猪5头，并设置空白对照组。通过观察和分析试验猪的临床表现、体温、增重、病毒血症、各组织的病毒载量和病理变化等方面评价仔猪感染试验结果。结果显示：PCV3阳性病料初始匀浆液和细胞培养物在感染断奶仔猪后均会引起仔猪出现体温上升、消瘦、皮炎和生长缓慢等现象；病毒血症时间超过14 d，但21 d消失，病毒的组织分布结果表明，PCV3主要感染扁桃体、淋巴结等免疫器官，在脾、肺和扁桃体等组织中的病毒载量最高；感染猪可出现间质性肺炎、淋巴小结增生和嗜酸性粒细胞增多等病理变化。本研究表明PCV3/CH/HB/XY/2018毒株对断奶仔猪有致病性，为PCV3的致病机制研究奠定了基础。

关键词: 猪圆环病毒3型, 分离, 致病性, 病毒载量

Abstract: Porcine circovirus type 3 (PCV3) is a new genotype of porcine circovirus firstly reported cause reproductive in sows in the United States in 2016, pathogenesis is not fully known. In order to study the pathogenicity of PCV3 in weaned piglets, a PCV3 strain was isolated using PK-15 cells from clinical tissue samples in this study and was named as PCV3/CH/HB/XY/2018, which belonged to PCV3a-IM genotype indicated by whole genome sequencing (WGS). Groups of five 28-day old weaned piglets were infected by both nasal inoculation and intramuscular injection with PCV3 positive tissue homogenate and the 8^th generation virus culture, respectively, meanwhile, a blank control group was set up. The results of infection test in piglets were evaluated in terms of clinical manifestation, body temperature, weight gain, viremia, tissue distribution and pathological changes. The results showed that after infection with PCV3 positive tissue homogenate or virus culture, the piglets in both groups showed high body temperature, emaciation, dermatitis and slow growth, and viremia lasted 14 days but disappear before 21 days post infection. Meanwhile, PCV3 was mainly found in tonsils and lymph nodes, and the highest viral load of PCV3 was found in spleen, lung and tonsils, the pathological changes in the lungs were characterized by interstitial pneumonia, lymphoid nodular hyperplasia and eosinophilia. This study proved that PCV3/CH/HB/XY/2018 was pathogenic to weaned piglets, and the isolation of this virus laid a foundation for the PCV3's pathogenesis mechanism research of PCV3-associate disease.

Key words: porcine circovirus type 3, isolation, pathogenicity, viral load

中图分类号:

S852.659.2

库旭钢, 于学祥, 孙琪, 李盼盼, 张梦佳, 罗锐, 钱平, 何启盖. 猪圆环病毒3型的分离及致病性分析[J]. 畜牧兽医学报, 2023, 54(4): 1568-1578.

KU Xugang, YU Xuexiang, SUN Qi, LI Panpan, ZHANG Mengjia, LUO Rui, QIAN Ping, HE Qigai. Isolation and Pathogenicity Analysis of Porcine Circovirus Type 3[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1568-1578.

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猪圆环病毒3型的分离及致病性分析

Isolation and Pathogenicity Analysis of Porcine Circovirus Type 3

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