2017—2021年成都市猫细小病毒的检测及其VP2基因的变异分析

doi:10.11843/j.issn.0366-6964.2022.04.031

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (4): 1303-1309.doi: 10.11843/j.issn.0366-6964.2022.04.031

2017—2021年成都市猫细小病毒的检测及其VP2基因的变异分析

周群¹, 杨苑¹, 宋鑫¹, 何欣怡¹, 曹慧¹, 张斌^1,2*

1. 西南民族大学畜牧兽医学院, 成都 610041;
2. 青藏高原动物遗传资源保护与利用教育部/四川省重点实验室, 成都 610041

收稿日期:2021-06-28 出版日期:2022-04-23 发布日期:2022-04-25
通讯作者: 张斌,主要从事动物病原分子生物学研究,E-mail:binovy@sina.com
作者简介:周群(1995-),女,四川遂宁人,主要从事动物病原分子生物学研究,E-mail:2297248747@qq.com
基金资助:
四川应用基础研究计划(2020YJ0247)；西南民族大学入选人才计划配套项目(RQD2021004)

Detection of Feline Parvovirus and Mutation Analysis of VP2 Gene in Chengdu from 2017 to 2021

ZHOU Qun¹, YANG Yuan¹, SONG Xin¹, HE Xinyi¹, CAO Hui¹, ZHANG Bin^1,2*

1. College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China;
2. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education/Sichuan Provine, Chengdu 610041, China

Received:2021-06-28 Online:2022-04-23 Published:2022-04-25

摘要/Abstract

摘要： 本研究旨在通过对成都地区家养猫细小病毒(feline parvovirus,FPV)的分子检测，了解FPV在成都地区的流行及遗传变异情况。2017-2021年分别从四川成都地区采集168份家养猫的腹泻粪便样本，用PCR方法对168份样本进行FPV检测，并选取13份阳性样本进行VP2基因全长的克隆和测序，用MegAlign软件分析FPV核苷酸和氨基酸序列的同源性及变异，通过MEGA 7.0软件用邻近法构建系统进化树分析成都地区FPV的遗传进化情况。结果表明，168份猫样本中检出FPV阳性样本118份，阳性率为70.2%。本研究获得的13株FPV VP2基因与参考毒株之间的同源性为97.1%~100%。VP2蛋白氨基酸分析表明，SMU-D18和SMU-D14毒株序列与new CPV-2a一致，为猫源犬细小毒株。有趣的是，本研究中SMU-D46毒株在第293和297位氨基酸均出现了独特的由Ser (S)到Phe (F)的替换。此外，系统进化分析显示，国内外FPV毒株在进化树中聚类为3个基因群(G1、G2和G3)，本研究获得的毒株分别位于G1和G3群中，值得注意的是，商品化的FPV疫苗株位于G2群中，与我国流行的FPV毒株亲缘关系较远。本研究表明，成都市猫群中FPV的流行十分普遍，急需重新评估现有的商品化疫苗的保护效率。

关键词: 猫细小病毒, 分子流行病学, VP2基因, 变体

Abstract: In order to investigate the prevalence and genetic variation of feline parvovirus (FPV) in domestic cats in Chengdu region. From 2017 to 2021, 168 diarrheal fecal samples were collected from domestic cats in Chengdu region of Sichuan province. FPV was detected in 168 samples, and 13 positive samples’nucleotide sequence of VP2 genes were obtained. The homology and variation of FPV nucleotide and amino acid (aa) sequences was analyzed by MegAlign software, and the phylogenetic tree was constructed by neighbor-joining method through MEGA 7.0. Of the 168 samples examined, 70.2% (118/168, 95% CI=62.7%-77.0%) samples were positive for FPV. Sequence homology analysis showed that the 13 full-length VP2 genes shared 97.1% to 100% homology to reference strains. The aa sequence of SMU-D18 and SMU-D14 strains were consistent with new CPV-2a from cats. Interestingly, the SMU-D46 strain showed a unique substitution from Ser (S) to Phe (F) at both 293aa and 297aa. Furthermore, phylogenetic analysis showed that the FPV strains from China and other countries clustered into three subgroups (G1, G2 and G3). The FPV isolates obtained in this study were located in G1 and G3, respectively. It was worth noting that the commercial FPV vaccine strain was located in G2, which had distantly related to the FPV strains circulating in China. The results demonstrated a very high infectious rate of FPV among cats in Chengdu region. The protective efficiency of the existing commercial vaccines should be re-evaluated as soon as possible.

Key words: feline parvovirus, molecular epidemiology, VP2 gene, variant

中图分类号:

周群, 杨苑, 宋鑫, 何欣怡, 曹慧, 张斌. 2017—2021年成都市猫细小病毒的检测及其VP2基因的变异分析[J]. 畜牧兽医学报, 2022, 53(4): 1303-1309.

ZHOU Qun, YANG Yuan, SONG Xin, HE Xinyi, CAO Hui, ZHANG Bin. Detection of Feline Parvovirus and Mutation Analysis of VP2 Gene in Chengdu from 2017 to 2021[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1303-1309.

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2017—2021年成都市猫细小病毒的检测及其VP2基因的变异分析

Detection of Feline Parvovirus and Mutation Analysis of VP2 Gene in Chengdu from 2017 to 2021

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