博落回提取物和抗生素联合用药对蛋种鸡自然感染滑液囊支原体的治疗效果分析

doi:10.11843/j.issn.0366-6964.2023.11.036

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (11): 4827-4838.doi: 10.11843/j.issn.0366-6964.2023.11.036

博落回提取物和抗生素联合用药对蛋种鸡自然感染滑液囊支原体的治疗效果分析

王晨燕¹, 周峰², 邵国青^1,3, 侯博^1*

1. 福建省农业科学院畜牧兽医研究所/福建省畜禽疫病疾病防治工程技术研究中心, 福州 350013;
2. 佛山科学技术学院生命科学与工程学院, 佛山 528231;
3. 江苏省农业科学院兽医研究所, 南京 210014

收稿日期:2023-04-06 出版日期:2023-11-23 发布日期:2023-11-26
通讯作者: 侯博,主要从事兽医传染病学研究,E-mail:houbohouwei@126.com
作者简介:王晨燕(1988-),女,福建福州人,硕士,助理研究员,主要从事兽医传染病学研究,E-mail:15396022617@163.com
基金资助:
福建省属公益类科研院所基本科研专项(2023R11010032);福建省农业科学院科技创新团队(CXTD2021014-3);福建省农业科学院"5511"协同创新重点项目(XTCXGC2021008);福建省农业科学院东西部协作项目(DKBF-2023-9)

Analysis of the Effect of Macleaya Cordata Extract in Conjunction with Antibiotics Treatment on the Natural Infection of Mycoplasma Synovia in Commercial Breeding Hens

WANG Chenyan¹, ZHOU Feng², SHAO Guoqing^1,3, HOU Bo^1*

1. Fujian Animal Diseases Control Technology Development Center, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
2. College of Life Science and Engineering, Foshan University, Foshan 528231, China;
3. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2023-04-06 Online:2023-11-23 Published:2023-11-26

摘要/Abstract

摘要： 旨在分析博落回提取物和抗生素联合用药对蛋种鸡滑液囊支原体(Mycoplasma synovia, MS)自然感染病例的治疗效果。选取80只124日龄的海兰褐父母代蛋种鸡,随机分为博落回提取物组(G1)、无处理组(G2)、盐酸多西环素+博落回提取物组(G3)、林可-大观霉素+博落回提取物组(G4)、酒石酸泰乐菌素+博落回提取物组(G5)、酒石酸泰万菌素+博落回提取物组(G6)、磷酸替米考星+博落回提取物组(G7)、延胡索酸泰妙菌素+博落回提取物组(G8)。G2组正常饮食和饮水,其余各组饲料中全程添加博落回提取物,此外G3~G8组通过饮水进行抗生素给药,连续使用5 d,停3 d后,再使用5 d。各组在用药前以及用药后第17天、第24天、第38天和第59天分别采集上颚裂拭子,并在用药后第59天采集输卵管膨大部和子宫部拭子进行MS病原荧光定量PCR检测。各组在用药前以及用药后第17天、第31天和第59天分别采血进行MS抗体检测。在用药后第59天剖检观察并统计包括气囊炎、脚垫肿胀和输卵管炎的发病率,同时采集气管、输卵管膨大部和子宫部固定并进行病理损伤观察。开产后,统计 6周的周日均产蛋数,并测定各组第4、5和6周的蛋品质。结果显示:1)MS病原检测结果表明:用药后第17天和第24天,G4组的上颚裂拭子MS载量相较于G2组分别极显著和显著下降(P＜0.001和P＜0.01),用药后第17天,G6和G7组的上颚裂拭子MS载量相较于G2组显著下降(P＜0.01);在用药后第17天和第24天,用药组上颚裂拭子的MS病原阳性率(G3和G5组除外)均下降,在用药后第59天,用药组(G4、G6和G8组除外)阳性率都恢复到用药前水平;G2和G8组的输卵管膨大部中MS病原阳性率为20%,G4、G5和G7组的阳性率均为10%,而G1、G3和G6组的输卵管膨大部以及各组子宫部均未检测到MS病原。2)MS抗体检测结果表明:G1和G4组用药后第59天抗体平均滴度较用药前下降60%左右。3)病理损伤观察发现:各组鸡用药前后气管均表现为不同程度的炎性细胞浸润,输卵管未发现任何病理损伤。4)蛋品质测定结果表明:G1、G3、G4和G6组开产后的周日均产蛋量都较G2组高;G8组开产后第4周的平均蛋重显著低于G2组(P＜0.01),其余各组均未发现有显著差异;G4组在产蛋后第6周的中蛋(53＜n≤63g)比率(60%)较其他组更高。综上,为了治疗蛋种鸡的MS自然感染,开产前采用博落回提取物或者博落回提取物联合抗生素,且开产后持续添加博落回提取物可降低上颚裂拭子的病原载量和阳性率,以及血清MS抗体平均滴度,同时周日均产蛋数明显增加,此外抗生素和博落回提取物联用还可提高中蛋的相对丰度。因此,本研究中的药物治疗方案可在一定程度上改善MS自然感染对蛋种鸡产蛋前期生产性能的影响。

关键词: 滑液囊支原体, 抗生素, 博落回, 药物治疗

Abstract: The purpose of this study was to analyse the effect of Macleaya cordata extract and its combination with antibiotics on the Mycoplasma synovia natural infection in breeding hens. Eighty breeding hens of Hy-line Brown Parent with 124-day-old were randomly divided into Macleaya cordata extract group (G1), non-treated group (G2), doxycycline hydrochloride + Macleaya cordata extract group (G3), linco-spectin+ Macleaya cordata extract group (G4), tylosin tartrate + Macleaya cordata extract group (G5), tylvalosin + Macleaya cordata extract group (G6), temicoxacin phosphate + Macleaya cordata extract group (G7), and tiamulin fumarate + Macleaya cordata extract group (G8), respectively. G2 hens were treated normally, Macleaya cordata extract were added to the feed throughout the experiment in all groups except G2, antibiotics were added to the drinking water in the group 3, 4, 5, 6, 7, 8 for 5 days, and added for another 5 days after 3 days withdrawal. The swabs of the cleft palate before treatment, on day 17, 24, 38 and 59 after treatment, the swab of the magnum and the uterus of the oviduct on day 59 after treatment were collected for MS detection by fluorescent quantitative PCR. The blood before treatment, on day 17, 31 and 59 after treatment was collected for the MS antibody detection. On day 59 after treatment, the morbidities of the air sacculitis, foot pad swelling and salpingitis were counted by dissection, and the trachea, the magnum and the uterus of the oviduct were harvested, fixed and sujected to histopathological observation. After delivery, the average daily egg production per week for each group was counted for 6 weeks, and egg quality was measured at the 4th, 5th and 6th week. The results showed that: 1) Results of MS antigen dectection: MS loads of cleft palate swabs in G4 on day 17 and 24 after treatment were extremely significantly and significantly lower than those of G2 (P＜0.001 and P＜0.01), respectively, and MS loads of cleft palate swabs in G6 and G7 on day 17 after treatment were significantly lower than that of G2 (P＜0.01); MS positive rate of cleft palate swabs in the treatment groups (except G3 and G5) decreased on the 17th or 24th day after treatment, and increased to the level before treatment (except G4, G6 and G8) on the 59th day; The positive rate of MS antigen of the magnum of the oviduct in G2 and G8 was 20%, in G4, G5 and G7 was 10%, no MS antigen was found in the the magnum in G1, G3 and G6 and none in uterus of all groups. 2) Results of MS antibody detection: The average titer of MS antibody in G1 and G4 decreased by about 60% on the 59th day after treatment compared with those before treatment. 3) Results of pathological lesions observation: Varying degrees of infiltrating inflanmatory cells were found in the trachea before and after treatment, and no histopathological lesions were observed in the ovduct. 4) Results of egg quality: The average daily egg production per week of G1, G3, G4 and G6 was higher than G2 group; The average daily egg production per week of G8 was significantly lower than that of G2 at the 4th week after delivery (P＜0.01), no significant difference was found in the egg weight and egg quality between groups at other time points; The proportion of medium egg (53＜n≤63) of G4 was higher than other groups at the 6th week (60%) after laying. In conclusion, for the MS natural infection, the use of Macleaya cordata extract alone or Macleaya cordata extract in conjunction with antibiotics before delivery, and the continuous use of Macleaya cordata extract after delivery could decrease the antigen loads, the positive rates of the cleft palate swabs and the average titer of MS antibody in serum, and also increase the average daily egg production per week. Furthermore, the use of Macleaya cordata extract in conjunction with antibiotics also improve the relative abundance of medium egg. Therefore, the medical treatments used in the current study could do some extent improve to the early production performance of breeding hens.

Key words: Mycoplasma Synoviae, antibiotics, Macleaya cordata, medical treatment

中图分类号:

S854.5

王晨燕, 周峰, 邵国青, 侯博. 博落回提取物和抗生素联合用药对蛋种鸡自然感染滑液囊支原体的治疗效果分析[J]. 畜牧兽医学报, 2023, 54(11): 4827-4838.

WANG Chenyan, ZHOU Feng, SHAO Guoqing, HOU Bo. Analysis of the Effect of Macleaya Cordata Extract in Conjunction with Antibiotics Treatment on the Natural Infection of Mycoplasma Synovia in Commercial Breeding Hens[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4827-4838.

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博落回提取物和抗生素联合用药对蛋种鸡自然感染滑液囊支原体的治疗效果分析

Analysis of the Effect of Macleaya Cordata Extract in Conjunction with Antibiotics Treatment on the Natural Infection of Mycoplasma Synovia in Commercial Breeding Hens

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