博落回提取物和抗生素联合用药对蛋种鸡自然感染滑液囊支原体的治疗效果分析

doi:10.11843/j.issn.0366-6964.2023.11.036

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (11): 4827-4838.doi: 10.11843/j.issn.0366-6964.2023.11.036

博落回提取物和抗生素联合用药对蛋种鸡自然感染滑液囊支原体的治疗效果分析

王晨燕¹, 周峰², 邵国青^1,3, 侯博^1*

1. 福建省农业科学院畜牧兽医研究所/福建省畜禽疫病疾病防治工程技术研究中心, 福州 350013;
2. 佛山科学技术学院生命科学与工程学院, 佛山 528231;
3. 江苏省农业科学院兽医研究所, 南京 210014

收稿日期:2023-04-06 出版日期:2023-11-23 发布日期:2023-11-26
通讯作者: 侯博,主要从事兽医传染病学研究,E-mail:houbohouwei@126.com
作者简介:王晨燕(1988-),女,福建福州人,硕士,助理研究员,主要从事兽医传染病学研究,E-mail:15396022617@163.com
基金资助:
福建省属公益类科研院所基本科研专项(2023R11010032);福建省农业科学院科技创新团队(CXTD2021014-3);福建省农业科学院"5511"协同创新重点项目(XTCXGC2021008);福建省农业科学院东西部协作项目(DKBF-2023-9)

Analysis of the Effect of Macleaya Cordata Extract in Conjunction with Antibiotics Treatment on the Natural Infection of Mycoplasma Synovia in Commercial Breeding Hens

WANG Chenyan¹, ZHOU Feng², SHAO Guoqing^1,3, HOU Bo^1*

1. Fujian Animal Diseases Control Technology Development Center, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
2. College of Life Science and Engineering, Foshan University, Foshan 528231, China;
3. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2023-04-06 Online:2023-11-23 Published:2023-11-26

摘要/Abstract

摘要： 旨在分析博落回提取物和抗生素联合用药对蛋种鸡滑液囊支原体(Mycoplasma synovia, MS)自然感染病例的治疗效果。选取80只124日龄的海兰褐父母代蛋种鸡,随机分为博落回提取物组(G1)、无处理组(G2)、盐酸多西环素+博落回提取物组(G3)、林可-大观霉素+博落回提取物组(G4)、酒石酸泰乐菌素+博落回提取物组(G5)、酒石酸泰万菌素+博落回提取物组(G6)、磷酸替米考星+博落回提取物组(G7)、延胡索酸泰妙菌素+博落回提取物组(G8)。G2组正常饮食和饮水,其余各组饲料中全程添加博落回提取物,此外G3~G8组通过饮水进行抗生素给药,连续使用5 d,停3 d后,再使用5 d。各组在用药前以及用药后第17天、第24天、第38天和第59天分别采集上颚裂拭子,并在用药后第59天采集输卵管膨大部和子宫部拭子进行MS病原荧光定量PCR检测。各组在用药前以及用药后第17天、第31天和第59天分别采血进行MS抗体检测。在用药后第59天剖检观察并统计包括气囊炎、脚垫肿胀和输卵管炎的发病率,同时采集气管、输卵管膨大部和子宫部固定并进行病理损伤观察。开产后,统计 6周的周日均产蛋数,并测定各组第4、5和6周的蛋品质。结果显示:1)MS病原检测结果表明:用药后第17天和第24天,G4组的上颚裂拭子MS载量相较于G2组分别极显著和显著下降(P＜0.001和P＜0.01),用药后第17天,G6和G7组的上颚裂拭子MS载量相较于G2组显著下降(P＜0.01);在用药后第17天和第24天,用药组上颚裂拭子的MS病原阳性率(G3和G5组除外)均下降,在用药后第59天,用药组(G4、G6和G8组除外)阳性率都恢复到用药前水平;G2和G8组的输卵管膨大部中MS病原阳性率为20%,G4、G5和G7组的阳性率均为10%,而G1、G3和G6组的输卵管膨大部以及各组子宫部均未检测到MS病原。2)MS抗体检测结果表明:G1和G4组用药后第59天抗体平均滴度较用药前下降60%左右。3)病理损伤观察发现:各组鸡用药前后气管均表现为不同程度的炎性细胞浸润,输卵管未发现任何病理损伤。4)蛋品质测定结果表明:G1、G3、G4和G6组开产后的周日均产蛋量都较G2组高;G8组开产后第4周的平均蛋重显著低于G2组(P＜0.01),其余各组均未发现有显著差异;G4组在产蛋后第6周的中蛋(53＜n≤63g)比率(60%)较其他组更高。综上,为了治疗蛋种鸡的MS自然感染,开产前采用博落回提取物或者博落回提取物联合抗生素,且开产后持续添加博落回提取物可降低上颚裂拭子的病原载量和阳性率,以及血清MS抗体平均滴度,同时周日均产蛋数明显增加,此外抗生素和博落回提取物联用还可提高中蛋的相对丰度。因此,本研究中的药物治疗方案可在一定程度上改善MS自然感染对蛋种鸡产蛋前期生产性能的影响。

关键词: 滑液囊支原体, 抗生素, 博落回, 药物治疗

Abstract: The purpose of this study was to analyse the effect of Macleaya cordata extract and its combination with antibiotics on the Mycoplasma synovia natural infection in breeding hens. Eighty breeding hens of Hy-line Brown Parent with 124-day-old were randomly divided into Macleaya cordata extract group (G1), non-treated group (G2), doxycycline hydrochloride + Macleaya cordata extract group (G3), linco-spectin+ Macleaya cordata extract group (G4), tylosin tartrate + Macleaya cordata extract group (G5), tylvalosin + Macleaya cordata extract group (G6), temicoxacin phosphate + Macleaya cordata extract group (G7), and tiamulin fumarate + Macleaya cordata extract group (G8), respectively. G2 hens were treated normally, Macleaya cordata extract were added to the feed throughout the experiment in all groups except G2, antibiotics were added to the drinking water in the group 3, 4, 5, 6, 7, 8 for 5 days, and added for another 5 days after 3 days withdrawal. The swabs of the cleft palate before treatment, on day 17, 24, 38 and 59 after treatment, the swab of the magnum and the uterus of the oviduct on day 59 after treatment were collected for MS detection by fluorescent quantitative PCR. The blood before treatment, on day 17, 31 and 59 after treatment was collected for the MS antibody detection. On day 59 after treatment, the morbidities of the air sacculitis, foot pad swelling and salpingitis were counted by dissection, and the trachea, the magnum and the uterus of the oviduct were harvested, fixed and sujected to histopathological observation. After delivery, the average daily egg production per week for each group was counted for 6 weeks, and egg quality was measured at the 4th, 5th and 6th week. The results showed that: 1) Results of MS antigen dectection: MS loads of cleft palate swabs in G4 on day 17 and 24 after treatment were extremely significantly and significantly lower than those of G2 (P＜0.001 and P＜0.01), respectively, and MS loads of cleft palate swabs in G6 and G7 on day 17 after treatment were significantly lower than that of G2 (P＜0.01); MS positive rate of cleft palate swabs in the treatment groups (except G3 and G5) decreased on the 17th or 24th day after treatment, and increased to the level before treatment (except G4, G6 and G8) on the 59th day; The positive rate of MS antigen of the magnum of the oviduct in G2 and G8 was 20%, in G4, G5 and G7 was 10%, no MS antigen was found in the the magnum in G1, G3 and G6 and none in uterus of all groups. 2) Results of MS antibody detection: The average titer of MS antibody in G1 and G4 decreased by about 60% on the 59th day after treatment compared with those before treatment. 3) Results of pathological lesions observation: Varying degrees of infiltrating inflanmatory cells were found in the trachea before and after treatment, and no histopathological lesions were observed in the ovduct. 4) Results of egg quality: The average daily egg production per week of G1, G3, G4 and G6 was higher than G2 group; The average daily egg production per week of G8 was significantly lower than that of G2 at the 4th week after delivery (P＜0.01), no significant difference was found in the egg weight and egg quality between groups at other time points; The proportion of medium egg (53＜n≤63) of G4 was higher than other groups at the 6th week (60%) after laying. In conclusion, for the MS natural infection, the use of Macleaya cordata extract alone or Macleaya cordata extract in conjunction with antibiotics before delivery, and the continuous use of Macleaya cordata extract after delivery could decrease the antigen loads, the positive rates of the cleft palate swabs and the average titer of MS antibody in serum, and also increase the average daily egg production per week. Furthermore, the use of Macleaya cordata extract in conjunction with antibiotics also improve the relative abundance of medium egg. Therefore, the medical treatments used in the current study could do some extent improve to the early production performance of breeding hens.

Key words: Mycoplasma Synoviae, antibiotics, Macleaya cordata, medical treatment

中图分类号:

S854.5

王晨燕, 周峰, 邵国青, 侯博. 博落回提取物和抗生素联合用药对蛋种鸡自然感染滑液囊支原体的治疗效果分析[J]. 畜牧兽医学报, 2023, 54(11): 4827-4838.

WANG Chenyan, ZHOU Feng, SHAO Guoqing, HOU Bo. Analysis of the Effect of Macleaya Cordata Extract in Conjunction with Antibiotics Treatment on the Natural Infection of Mycoplasma Synovia in Commercial Breeding Hens[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4827-4838.

参考文献 34

[1]	CATANIA S,GOBBO F,BILATO D,et al.Two strains of Mycoplasma synoviae from chicken flocks on the same layer farm differ in their ability to produce eggshell apex abnormality[J].Vet Microbiol,2016,193:60-66.
[2]	SENTÍES-CUÉ G,SHIVAPRASAD H L,CHIN R P.Systemic Mycoplasma synoviae infection in broiler chickens[J].Avian Pathol,2005,34(2):137-142.
[3]	KLEVEN S H,FERGUSON-NOEL N,RAVIV Z,et al.Serological responses of chickens to low challenge doses of Mycoplasma synoviae[J].Avian Dis,2007,51(3):738-743.
[4]	SUN S K,LIN X,CHEN F,et al.Epidemiological investigation of Mycoplasma Synoviae in native chicken breeds in China[J].BMC Vet Res,2017,13(1):115.
[5]	XUE J,XU M Y,MA Z J,et al.Serological investigation of Mycoplasma synoviae infection in China from 2010 to 2015[J].Poult Sci,2017,96(9):3109-3112.
[6]	GOLE V C,CHOUSALKAR K K,ROBERTS J R.Prevalence of antibodies to Mycoplasma synoviae in laying hens and possible effects on egg shell quality[J].Prev Vet Med,2012,106(1):75-78.
[7]	FEBERWEE A,DE WIT J J,LANDMAN W J M.Induction of eggshell apex abnormalities by Mycoplasma synoviae:field and experimental studies[J].Avian Pathol,2009,38(1):77-85.
[8]	FEBERWEE A,DIJKMAN R,KLINKENBERG D,et al.Quantification of the horizontal transmission of Mycoplasma synoviae in non-vaccinated and MS-H-vaccinated layers[J].Avian Pathol,2017,46(4):346-358.
[9]	FEBERWEE A,MORROW C J,GHORASHI S A,et al.Effect of a live Mycoplasma synoviae vaccine on the production of eggshell apex abnormalities induced by a M. synoviae infection preceded by an infection with infectious bronchitis virus D1466[J].Avian Pathol,2009,38(5):333-340.
[10]	JONES J F,WHITHEAR K G,SCOTT P C,et al.Determination of the effective dose of the live Mycoplasma synoviae vaccine,vaxsafe MS (strain MS-H) by protection against experimental challenge[J].Avian Dis,2006,50(1):88-91.
[11]	GONG X W,CHEN Q W,FERGUSON-NOEL N,et al.Evaluation of protective efficacy of inactivated Mycoplasma synoviae vaccine with different adjuvants[J].Vet Immunol Immunopathol,2020,220:109995.
[12]	EWING M L,COOKSON K C,PHILLIPS R A,et al.Experimental infection and transmissibility of Mycoplasma synoviae with delayed serologic response in chickens[J].Avian Dis,1998,42(2):230-238.
[13]	王晨燕,罗忠宝,黄宝钦,等.福建省2018年~2020年非免疫鸡群滑液囊支原体感染的流行病学调查[J].中国预防兽医学报,2022,44(7):697-703.WANG C Y,LUO Z B,HUANG B Q,et al.Investigation of Mycoplasma synoviae infection in unvaccinated chicken flocks during 2018—2020 in Fujian[J].Chinese Journal of Preventive Veterinary Medicine,2022,44(7):697-703.(in Chinese)
[14]	马爽,郭莉莉,宋新宇,等.鸡滑液囊支原体感染的流行病学调查与分析[J].中国家禽,2016,38(23):68-71.MA S,GUO L L,SONG X Y,et al.Epidemiological investigation and analysis of Mycoplasma synoviae infection[J].China Poultry,2016,38(23):68-71.(in Chinese)
[15]	MORROW C J,KREIZINGER Z,ACHARI R R,et al.Antimicrobial susceptibility of pathogenic mycoplasmas in chickens in Asia[J].Vet Microbiol,2020,250:108840.
[16]	BUIM M R,BUZINHANI M,YAMAGUTI M,et al.Mycoplasma synoviae cell invasion:elucidation of the Mycoplasma pathogenesis in chicken[J].Comp Immunol Microbiol,2011,34(1):41-47.
[17]	HONG Y H,KWON J S,LEE H J,et al.Eradication of Mycoplasma synoviae from a multi-age broiler breeder farm using antibiotics therapy[J].Poult Sci,2015,94(10):2364-2368.
[18]	XUE G D,WU S B,CHOCT M,et al.Impact of a Macleaya cordata-derived alkaloid extract on necrotic enteritis in broilers[J].Poult Sci,2017,96(10):3581-3585.
[19]	GUO S C,LEI J X,LIU L L,et al.Effects of Macleaya cordata extract on laying performance,egg quality,and serum indices in Xuefeng black-bone chicken[J].Poult Sci,2021,100(4):101031.
[20]	NIU X F,FAN T,LI W F,et al.The anti-inflammatory effects of sanguinarine and its modulation of inflammatory mediators from peritoneal macrophages[J].Eur J Pharmacol,2012,689(1-3):262-269.
[21]	CHATURVEDI M M,KUMAR A,DARNAY B G,et al.Sanguinarine (pseudochelerythrine) is a potent inhibitor of NF-κB activation,IκBα phosphorylation,and degradation[J].J Biol Chem,1997,272(48):30129-30134.
[22]	HU Z W,HU H,HU Z L,et al.Sanguinarine,isolated from Macleaya cordata,exhibits potent antifungal efficacy against Candida albicans through inhibiting ergosterol synthesis[J].Front Microbiol,2022,13:908461.
[23]	LU C,ZHANG N,KOU S,et al.Sanguinarine synergistically potentiates aminoglycoside-mediated bacterial killing[J].Microb Biotechnol,2022,15(7):2055-2070.
[24]	中华人民共和国农业部.NY/T 553—2015 禽支原体PCR检测方法[S].北京:中国农业出版社,2015.Ministry of Agriculture of the PRC.NY/T 553—2015 Detection of avian Mycoplasmas by polymerase chain reaction (PCR)[S].Beijing:China Agriculture Press,2015.(in Chinese)
[25]	SUN S K,LIN X,LIU J M,et al.Phylogenetic and pathogenic analysis of Mycoplasma Synoviae isolated from native chicken breeds in China[J].Poult Sci,2017,96(7):2057-2063.
[26]	DUFOUR-GESBERT F,DHEILLY A,MAROIS C,et al.Epidemiological study on Mycoplasma synoviae infection in layers[J].Vet Microbiol,2006,114(1-2):148-154.
[27]	ZHANG X R,GUO M J,XIE D,et al.Antibiotic resistance of Mycoplasma Synoviae strains isolated in China from 2016 to 2019[J].BMC Vet Res,2022,18(1):1.
[28]	GAUTIER-BOUCHARDON A V,REINHARDT A K,KOBISCH M,et al.In vitro development of resistance to enrofloxacin,erythromycin,tylosin,tiamulin and oxytetracycline in Mycoplasma gallisepticum,Mycoplasma iowae and Mycoplasma synoviae[J].Vet Microbiol,2002,88(1):47-58.
[29]	KHIN M,JONES A M,CECH N B,et al.Phytochemical analysis and antimicrobial efficacy of Macleaya cordata against extensively drug-resistant Staphylococcus aureus[J].Nat Prod Commun,2018,13(11):10.
[30]	FIORENTIN L,SONCINI R A,DA COSTA J L A,et al.Apparent eradication of Mycoplasma synoviae in broiler breeders subjected to intensive antibiotic treatment directed to control Escherichia coli[J].Avian Pathol,2003,32(2):213-216.
[31]	STANLEY W A,HOFACRE C L,SPEKSNIJDER G,et al.Monitoring Mycoplasma gallisepticum and Mycoplasma synoviae infection in breeder chickens after treatment with enrofloxacin[J].Avian Dis,2001,45(2):534-539.
[32]	KURSA O,PAKUȽA A,TOMCZYK G,et al.Eggshell apex abnormalities caused by two different Mycoplasma synoviae genotypes and evaluation of eggshell anomalies by full-field optical coherence tomography[J].BMC Vet Res,2019,15(1):1.
[33]	CATANIA S,BILATO D,GOBBO F,et al.Treatment of eggshell abnormalities and reduced egg production caused by Mycoplasma synoviae infection[J].Avian Dis,2010,54(2):961-964.
[34]	JEON E O,KIM J N,LEE H R,et al.Eggshell apex abnormalities associated with Mycoplasma synoviae infection in layers[J].J Vet Sci,2014,15(4):579-582.

博落回提取物和抗生素联合用药对蛋种鸡自然感染滑液囊支原体的治疗效果分析

Analysis of the Effect of Macleaya Cordata Extract in Conjunction with Antibiotics Treatment on the Natural Infection of Mycoplasma Synovia in Commercial Breeding Hens

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 34

相关文章 15

编辑推荐

Metrics

本文评价

[1]	陶洁, 李本强, 程靖华, 石迎, 刘佩红, 刘惠莉. 肉鸽肠道微生物菌群差异分析和抗生素耐药基因预测[J]. 畜牧兽医学报, 2023, 54(12): 5293-5300.
[2]	孙盼盼, 曹志刚, 凌小雅, 孙娜, 孙耀贵, 李宏全. 苦参碱联合不同抗生素抗炎作用比较研究[J]. 畜牧兽医学报, 2023, 54(10): 4411-4421.
[3]	操庆国, 郭钦, 彭凯, 蔡佳惠, 任成万, 李昀庭. 盐酸小檗碱与左氧氟沙星联合抑制MRSA及其机制研究[J]. 畜牧兽医学报, 2022, 53(9): 3208-3220.
[4]	董嘉琪, 张旺东, 姚万玲, 薛姣, 刘莹发, 魏彦明, 纪鹏. 红芪多糖-1-1的分离纯化及其调节抗生素诱导小鼠肠道菌群失调的最佳剂量分析[J]. 畜牧兽医学报, 2022, 53(8): 2794-2811.
[5]	许李锋, 贾晨宇, 陈福再, 方梦园, 刘孝丹, 陈吉龙, 李训良. 1株鸡滑液囊支原体的分离鉴定与全基因组分析[J]. 畜牧兽医学报, 2022, 53(8): 2663-2676.
[6]	何绿琴, 闫雪锋, 文心田, 曹三杰, 黄小波, 伍锐, 赵勤, 文翼平. 副猪嗜血杆菌qseB、qseC双基因缺失株的构建及生物学特性[J]. 畜牧兽医学报, 2022, 53(2): 529-537.
[7]	刘丽佳, 王誉, 张焕容, 王嘉博. 7株鸡滑液囊支原体四川分离株全基因组分析[J]. 畜牧兽医学报, 2022, 53(2): 505-519.
[8]	秦明星, 宫晓炜, 陈启伟, 王燕萍, 权衡, 朱雨佳, 郑福英, 蔺国珍. 鸭疫里默氏杆菌RA-LZ01株GE296_RS01450基因介导其对重金属离子产生耐受性的分析[J]. 畜牧兽医学报, 2021, 52(10): 2864-2873.
[9]	张仕泓, 王少林. 动物源产气荚膜梭菌耐药性研究进展[J]. 畜牧兽医学报, 2021, 52(10): 2762-2771.
[10]	戴青, 杨秀玉, 韩宁宁, 王轩, 赵晖. 泰万菌素兽用国家标准品的研制[J]. 畜牧兽医学报, 2020, 51(8): 1985-1992.
[11]	董朕, 陈晨, 李冰, 周绪正, 张继瑜. 糖肽类抗生素的研究进展[J]. 畜牧兽医学报, 2020, 51(7): 1488-1498.
[12]	吴香云, 刘亚娜, 周喆麒, 潘源虎, 郝海红, 程古月, 王玉莲. 多糖类化合物的抗菌作用及其机制研究进展[J]. 畜牧兽医学报, 2020, 51(6): 1167-1176.
[13]	赵佳琦, 文勇立, 安雅静, 李子谦, 齐沛森, 李强, 候定超. 牦牛瘤胃微生物抗生素抗性基因对3种外源性刺激因子的响应[J]. 畜牧兽医学报, 2020, 51(5): 1126-1137.
[14]	马强, 王艺晖, 常佳伟, 万佳宏, 魏彦琴, 王桂琴. 金黄色葡萄球菌细胞壁变化与β-内酰胺类抗生素耐药的关系[J]. 畜牧兽医学报, 2020, 51(3): 602-611.
[15]	佟盼盼, 马凯琪, 刘争辉, 谢金鑫, 苏红, 王栋, 孙雪, 高姣姣, 苏战强. 新疆牛源产志贺毒素大肠杆菌耐药性及其ESBLs基因鉴定[J]. 畜牧兽医学报, 2019, 50(4): 887-892.