蛋鸡低蛋白日粮研究进展

doi:10.11843/j.issn.0366-6964.2022.10.004

摘要/Abstract

摘要： 蛋白质饲料原料资源紧张和养殖氮排放污染是我国蛋鸡业面临的主要难题。为此，蛋鸡低蛋白日粮在近年来应运而生，逐渐成熟。相关研究多以标准回肠可消化氨基酸为依据来配制蛋鸡低蛋白日粮，以期减少蛋白质饲料原料消耗、降低养殖成本、减少氮排放。本文简述了低蛋白日粮技术的理论基础及其在蛋鸡上的研究进展，旨在为低蛋白饲料在蛋鸡生产上的应用提供参考。

关键词: 蛋鸡, 低蛋白日粮, 氨基酸平衡, 能量平衡, 功能性氨基酸, 蛋清品质

Abstract: Scarcity of feed protein source and nitrogen emission pollution are important problems in poultry industry. For this reason, low-protein diets for laying hens came into being in recent years and was gradually widely used. Low-protein diets of laying hens in recent studies were mainly formulated according to the standard ileal digestible amino acid content of feedstuffs, in order to reduce protein resources consumption, feeding cost, and nitrogen emission. Here, the theoretical basis of low-protein diet and its research progress in laying hens were briefly reviewed, aiming to improve the application of low-protein diet in laying hens.

Key words: laying hens, low-protein diets, amino acid balance, energy balance, functional amino acid, albumen quality

中图分类号:

S831.5

邱凯, 常心雨, 车彦卓, 王晶, 张海军, 齐广海, 武书庚. 蛋鸡低蛋白日粮研究进展[J]. 畜牧兽医学报, 2022, 53(10): 3305-3315.

QIU Kai,CHANG Xinyu,CHE Yanzhuo,WANG Jing,ZHANG Haijun,QI Guanghai,WU Shugeng. Research Advances on Low-Protein Diets of Laying Hens[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3305-3315.

参考文献 95

[1]	赵卿尧, 顾宪红.低蛋白日粮应用前景与研究进展[J].家畜生态学报, 2020, 41(3):9-14.ZHAO Q Y, GU X H.Application prospect and research progress of low protein diet[J].Acta Ecologae Animalis Domastici, 2020, 41(3):9-14.(in Chinese)
[2]	邵钰, 沈杰, 王志跃, 等.低蛋白饲粮在家禽生产中的研究与应用[J].中国饲料, 2021(15):64-67.SHAO Y, SHEN J, WANG Z Y, et al.Research and application of low protein diet in poultry production[J].China Feed, 2021(15):64-67.(in Chinese)
[3]	宋博, 尹杰, 郑昌炳, 等.低蛋白质日粮在畜禽生产中的应用研究进展[J].中国饲料, 2020(3):8-15.SONG B, YIN J, ZHENG C B, et al.Advances in application of low protein diets in livestock and poultry production[J].China Feed, 2020(3):8-15.(in Chinese)
[4]	郭丹.正常及低蛋白饲粮下产蛋高峰期蛋鸡蛋氨酸需要量的研究[D].哈尔滨:东北农业大学, 2016.GUO D.Research on methionine requirement under nomal protein and low protein diet for laying hens[D].Harbin:Northeast Agricultural University, 2016.(in Chinese)
[5]	任冰.理想氨基酸模式下低蛋白日粮对产蛋鸡生产性能及氨氮排放的影响[D].杨凌:西北农林科技大学, 2012.REN B.Effects of low protein diet in ideal amino acid pattern on laying performance and N excretion of laying Hens[D].Yangling:Northwest A&F University, 2012.(in Chinese)
[6]	刘庚, 武书庚, 计峰, 等.30~38周龄产蛋鸡理想氨基酸模式的研究[J].动物营养学报, 2012, 24(8):1447-1458.LIU G, WU S G, JI F, et al.Ideal amino acid pattern for laying hens aged thirty to thirty-eight weeks[J].Chinese Journal of Animal Nutrition, 2012, 24(8):1447-1458.(in Chinese)
[7]	ALAGAWANY M, EL-HINDAWY M M, EL-HACK M E A, et al.Influence of low-protein diet with different levels of amino acids on laying hen performance, quality and egg composition[J].An Acad Bras Ciênc, 2020, 92(1):e20180230.
[8]	齐广海, 岳洪源, 武书庚, 等.蛋鸡氨基酸营养的研究进展[J].动物营养学报, 2014, 26(10):3108-3113.QI G H, YUE H Y, WU S G, et al.Recent development on amino acid nutrition in laying hens[J].Chinese Journal of Animal Nutrition, 2014, 26(10):3108-3113.(in Chinese)
[9]	LEMME A, RAVINDRAN V, BRYDEN W L.Ileal digestibility of amino acids in feed ingredients for broilers[J].World's Poultry Sci J, 2004, 60(4):423-438.
[10]	STEIN H H, TROTTIER N L, BELLAVER C, et al.The effect of feeding level and physiological status on total flow and amino acid composition of endogenous protein at the distal ileum in swine[J].J Anim Sci, 1999, 77(5):1180-1187.
[11]	BREGENDAHL K, ROBERTS S A, KERR B, et al.Ideal ratios of isoleucine, methionine, methionine plus cystine, threonine, tryptophan, and valine relative to lysine for white leghorn-type laying hens of twenty-eight to thirty-four weeks of age[J].Poult Sci, 2008, 87(4):744-758.
[12]	LEMME A.Amino acid recommendations for laying hens[J].Lohmann Information, 2009, 44(2):21-31.
[13]	练泳洋.低蛋白饲料在蛋鸡生产有效利用及推广[J].中国畜禽种业, 2019, 15(10):181-182.LIAN Y Y.Effective utilization and promotion of low protein feed in layer production[J].The Chinese Livestock and Poultry Breeding, 2019, 15(10):181-182.(in Chinese)
[14]	陈晓慧, 孙冬艳, 王通, 等.低蛋白氨基酸平衡饲粮对蛋鸡育成阶段生长性能的影响[J].饲料工业, 2021, 42(18):24-28.CHEN X H, SUN D Y, WANG T, et al.Effects of low protein and amino acid balanced diet on growth performance of laying hens at growing stage[J].Feed Industry, 2021, 42(18):24-28.(in Chinese)
[15]	TORKI M, MOHEBBIFAR A, GHASEMI H A, et al.Response of laying hens to feeding low-protein amino acid-supplemented diets under high ambient temperature:performance, egg quality, leukocyte profile, blood lipids, and excreta pH[J].Int J Biometeorol, 2015, 59(5):575-84.
[16]	张健.产蛋鸡低蛋白饲粮主要必需氨基酸适宜水平研究[D].哈尔滨:东北农业大学, 2018.ZHANG J.Estimation of optimum requirements for major essential amino acids in low-protein, amino acid-supplemented diets foraying hens[D].Harbin:Northeast Agricultural University, 2018.(in Chinese)
[17]	GENG S H, HUANG S M, MA Q G, et al.Alterations and correlations of the gut microbiome, performance, egg quality, and serum biochemical indexes in laying hens with low-protein amino acid-deficient diets[J].ACS Omega, 2021, 6(20):13094-13104.
[18]	MA M L, GENG S J, LIU M L, et al.Effects of different methionine levels in low protein diets on production performance, reproductive system, metabolism, and gut microbiota in laying hens[J].Front Nutr, 2021, 8:739676.
[19]	SUN M F, ZHAO J P, WANG X J, et al.Use of encapsulated L-lysine-HCl and DL-methionine improves postprandial amino acid balance in laying hens[J].J Anim Sci, 2020, 98(10):skaa315.
[20]	张恒硕.低蛋白日粮对蛋鸡产蛋后期生产性能影响及营养调控技术研究[D].杭州:浙江大学, 2020.ZHANG H S.Effects of low protein diet on late production performance and nutritional regulation of laving lens[D].Hangzhou:Zhejiang University, 2020.(in Chinese)
[21]	WHITE D, ADHIKARI R, WANG J Q, et al.Effects of dietary protein, energy and β-mannanase on laying performance, egg quality, and ileal amino acid digestibility in laying hens[J].Poult Sci, 2021, 100(9):101312.
[22]	LESUISSE J, LI C, SCHALLIER S, et al.Multigenerational effects of a reduced balanced protein diet during the rearing and laying period of broiler breeders.1.Performance of the F1 breeder generation[J].Poult Sci, 2018, 97(5):1651-1665.
[23]	LI H, WANG T A, XU C L, et al.Transcriptome profile of liver at different physiological stages reveals potential mode for lipid metabolism in laying hens[J].BMC Genomics, 2015, 16(1):763.
[24]	ROZENBOIM I, MAHATO J, COHEN N A, et al.Low protein and high-energy diet:A possible natural cause of fatty liver hemorrhagic syndrome in caged White Leghorn laying hens[J].Poult Sci, 2016, 95(3):612-621.
[25]	姜家麟.高能低蛋白日粮致FLHS蛋鸡的血清代谢组学分析[D].南昌:江西农业大学, 2019.JIANG J L.Serum metabolomics analysis of laying hens with FLHs induced by high energy and low protein diet[D].Nanchang:Jiangxi Agricultural University, 2019.(in Chinese)
[26]	GAO X N, LIU P, WU C, et al.Effects of fatty liver hemorrhagic syndrome on the AMP-activated protein kinase signaling pathway in laying hens[J].Poult Sci, 2019, 98(5):2201-2210.
[27]	ZHUANG Y, XING C H, CAO H B, et al.Insulin resistance and metabonomics analysis of fatty liver haemorrhagic syndrome in laying hens induced by a high-energy low-protein diet[J].Sci Rep, 2019, 9(1):10141.
[28]	王玉洁, 刘佳, 魏庆, 等.高能低蛋白日粮诱导的脂肪肝出血综合征(FLHS)蛋鸡脂肪组织代谢差异的比较[J].中国兽医学报, 2020, 40(10):2061-2065.WANG Y J, LIU J, WEI Q, et al.Comparison of adipose tissue metabolism differences in fatty liver hemorrhage syndrome(FLHS) hens induced by high energy and low protein diet[J].Chinese Journal of Veterinary Science, 2020, 40(10):2061-2065.(in Chinese)
[29]	郭小权.高能低蛋白日粮致脂肪肝出血综合征鸡抗氧化能力和肝损伤的研究[D].南昌:江西农业大学, 2020.GUO X Q.Study on the antioxidant capacity and liver damage of fatty liver hemorrhagic syndrome chickens induced by high-energy and low-protein diet[D].Nanchang:Jiangxi Agricultural University, 2020.(in Chinese)
[30]	PENG G, HUANG E F, RUAN J M, et al.Effects of a high energy and low protein diet on hepatic and plasma characteristics and Cidea and Cidec mRNA expression in liver and adipose tissue of laying hens with fatty liver hemorrhagic syndrome[J].Anim Sci J, 2019, 90(2):247-254.
[31]	王子强.利用低蛋白净能拟合日粮对蛋鸡生产性能的影响研究[D].济南:山东农业大学, 2012.WANG Z Q.Effect of diet formulation with low protein and net energy on the laying performance of hens[D].Ji'nan:Shandong Agricultural University, 2012.(in Chinese)
[32]	何维明, 杨福有, 刘慧珍, 等.饲粮蛋白能量比值与产蛋鸡脂肪肝综合症发生率的关系研究[J].畜牧兽医学报, 1992, 23(2):107-111.HE W M, YANG F Y, LIU H Z, et al.Study on relationship between energy-protein ratio and frequency of fatty liver syndrome in laying hens[J].Acta Veterinaria et Zootechnica Sinica, 1992, 23(2):107-111.(in Chinese)
[33]	LI F, ZHANG L M, WU X H, et al.Effects of metabolizable energy and balanced protein on egg production, quality, and components of Lohmann Brown laying hens[J].J Appl Poultry Res, 2013, 22(1):36-46.
[34]	陆江, 朱道仙, 卢劲晔, 等.卵磷脂对饲喂高能低蛋白饲粮青年蛋鸡肝脏脂肪代谢的影响[J].中国家禽, 2018, 40(12):55-58.LU J, ZHU D X, LU J Y, et al.Effect and mechanism of lecithin on liver lipometabolism by high-energy diet in yong layers[J].China Poultry, 2018, 40(12):55-58.(in Chinese)
[35]	郭小权, 曹华斌, 胡国良, 等.高能量低蛋白质日粮中添加生物素对蛋鸡脂类代谢的影响[J].中国兽医学报, 2012, 32(5):754-758.GUO X Q, CAO H B, HU G L, et al.Effect of high-energy low-protein diet supplemented with biotin on fat metabolism of laying hens[J].Chinese Journal of Veterinary Science, 2012, 32(5):754-758.(in Chinese)
[36]	刘海隆, 崔静, 王令令, 等.鸡骨草提取液对高能低蛋白饲料饲喂蛋鸡血脂指标的影响[J].饲料研究, 2021, 44(8):41-44.LIU H L, CUI J, WANG L L, et al.Effect of Abrus cantoniensis Hance extract on blood lipid of laying hen feed with high energy and low protein diet[J].Feed Research, 2021, 44(8):41-44.(in Chinese)
[37]	陈毅燕.盐酸小檗碱对高能低蛋白日粮诱导的蛋鸡脂肪肝出血综合征防治作用研究[D].南昌:江西农业大学, 2020.CHEN Y Y.The prevention and treatment effect of berberine hydrochloride on fatty liver hemorrhagic syndrome in laying hens induced by high-energy and low-protein diet[D].Nanchang:Jiangxi Agricultural University, 2020.(in Chinese)
[38]	杨长进.苜草素对蛋鸡脂肪肝出血综合征预防效果的研究[D].杨凌:西北农林科技大学, 2015.YANG C J.Prophylactic effect of poly savone on fatty liver hemorrhagic syndrome in laying hens[D].Yangling:Northwest A&F University, 2015.(in Chinese)
[39]	OMER N A, HU Y, IDRISS A A, et al.Dietary betaine improves egg-laying rate in hens through hypomethylation and glucocorticoid receptor-mediated activation of hepatic lipogenesis-related genes[J].Poult Sci, 2020, 99(6):3121-3132.
[40]	BAVARSADI M, MAHDAVI A H, ANSARI-MAHYARI S, et al.Effects of different levels of sanguinarine on antioxidant indices, immunological responses, ileal microbial counts and jejunal morphology of laying hens fed diets with different levels of crude protein[J].J Anim Physiol Anim Nutr, 2017, 101(5):936-948.
[41]	BAVARSADI M, MAHDAVI A H, ANSARI-MAHYARI S, et al.Sanguinarine improved nutrient digestibility, hepatic health indices and productive performance in laying hens fed low crude protein diets[J].Vet Med Sci, 2021, 7(3):800-811.
[42]	WANG X Y, XING C, YANG F, et al.Abnormal expression of liver autophagy and apoptosis-related mRNA in fatty liver haemorrhagic syndrome and improvement function of resveratrol in laying hens[J].Avian Pathol, 2020, 49(2):171-178.
[43]	XING C H, WANG Y, DAI X Y, et al.The protective effects of resveratrol on antioxidant function and the mRNA expression of inflammatory cytokines in the ovaries of hens with fatty liver hemorrhagic syndrome[J].Poult Sci, 2020, 99(2):1019-1027.
[44]	QIU K, ZHAO Q, WANG J, et al.Effects of pyrroloquinoline quinone on lipid metabolism and anti-oxidative capacity in a high-fat-diet metabolic dysfunction-associated fatty liver disease chick model[J].Int J Mol Sci, 2021, 22(3):1458.
[45]	邓雪娟.肉仔鸡饲料原料可消化氨基酸和代谢能的生物学评定及可加性研究[D].北京:中国农业科学院, 2008.DENG X J.Study on additivity and biological value of digestible amino acid and metabolizable energy in ingredients for broilers[D].Beijing:Chinese Academy of Agricultural Sciences, 2008.(in Chinese)
[46]	付胜勇, 武书庚, 张海军, 等.饲粮代谢能和粗蛋白质水平对21~34周龄海兰灰蛋鸡生产性能与蛋品质的影响[J].动物营养学报, 2013, 25(11):2601-2611.FU S Y, WU S G, ZHANG H J, et al.Effects of dietary metabolizable energy and crude protein levels on performance and egg quality of Hy-Line laying hens aged 21 to 34 weeks[J].Chinese Journal of Animal Nutrition, 2013, 25(11):2601-2611.(in Chinese)
[47]	焦莉, 王春明, 王洪芳, 等.低营养水平日粮添加复合酶对蛋鸡生产性能、蛋品质和抗氧化的影响[J].中国饲料, 2021(6):63-66.JIAO L, WANG C M, WANG H F, et al.Effects of adding complex enzymes to low nutrient level diets on performance, egg quality and antioxidant activity of laying hens[J].China Feed, 2021(6):63-66.(in Chinese)
[48]	张利敏.氨基酸平衡饲粮的代谢能和粗蛋白质水平对产蛋鸡生产性能及养分利用的影响[D].杨凌:西北农林科技大学, 2009.ZHANG L M.Effects of dietary metabolizable energy and crude protein on performance and nutrient retention of laying hens[D].Yangling:Northwest A&F University, 2009.(in Chinese)
[49]	KIM S W, MATEO R D, YIN Y L, et al.Functional amino acids and fatty acids for enhancing production performance of sows and piglets[J].Asian-Australas J Anim Sci, 2007, 20(2):295-306.
[50]	WU G Y, BAZER F W, DAVIS T A, et al.Important roles for the arginine family of amino acids in swine nutrition and production[J].Livest Sci, 2007, 112(1-2):8-22.
[51]	侯海锋, 李茜.低蛋白质日粮添加色氨酸对蛋鸡生产性能、蛋品质及蛋白质代谢的影响[J].中国饲料, 2017(13):13-15, 23.HOU H F, LI Q.Effects of adding tryptophan in low-protein diets on production performance, egg quality and protein metabolism of laying hens[J].China Feed, 2017(13):13-15, 23.(in Chinese)
[52]	刘国花, 邹晓庭, 谢正军, 等.低蛋白饲粮添加苏氨酸对蛋鸡生产性能及蛋品质的影响[J].中国家禽, 2012, 34(9):33-36.LIU G H, ZOU X T, XIE Z J, et al.Effects of adding threonine in low-protein diet on production performance and egg quality in laying hens[J].China Poultry, 2012, 34(9):33-36.(in Chinese)
[53]	AZZAM M M M, DONG X Y, ZOU X T.Effect of dietary threonine on laying performance and intestinal immunity of laying hens fed low-crude-protein diets during the peak production period[J].J Anim Physiol Anim Nutr, 2017, 101(5):e55-e66.
[54]	DONG X Y, AZZAM M M M, ZOU X T.Effects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying hens[J].Poult Sci, 2017, 96(10):3654-3663.
[55]	AZZAM M M, ALHOTAN R, AL-ABDULLATIF A, et al.Threonine requirements in dietary low crude protein for laying hens under high-temperature environmental climate[J].Animals, 2019, 9(9):586.
[56]	OSPINA-ROJAS I G, MURAKAMI A, FANHANI J C, et al.Tryptophan, threonine and isoleucine supplementation in lowprotein diets for commercial laying hens[J].Semina:Ciências Agrárias, 2015, 36(3):1735-1743.
[57]	HARRIS R A, KOBAYASHI R, MURAKAMI T, et al.Regulation of branched-chain α-keto acid dehydrogenase kinase expression in Rat Liver[J].J Nutr, 2001, 131(3):841S-845S.
[58]	OSPINA-ROJAS I C, MURAKAMI A E, DUARTE C R A, et al.Leucine and valine supplementation of low-protein diets for broiler chickens from 21 to 42 days of age[J].Poult Sci, 2017, 96(4):914-922.
[59]	DONG X Y, AZZAM M M M, ZOU X T.Effects of dietary L-isoleucine on laying performance and immunomodulation of laying hens[J].Poult Sci, 2016, 95(10):2297-2305.
[60]	PARENTEAU I A, STEVENSON M, KIARIE E G.Egg production and quality responses to increasing isoleucine supplementation in Shaver white hens fed a low crude protein corn-soybean meal diet fortified with synthetic amino acids between 20 and 46 weeks of age[J].Poult Sci, 2020, 99(3):1444-1453.
[61]	STAMP D, JENKINS G J S.An overview of bile-acid synthesis, chemistry and function[J].Issues in Toxicology, 2008, doi:10.1039/9781847558336-00001.
[62]	HAN Y K, THACKER P A.Influence of energy level and glycine supplementation on performance, nutrient digestibility and egg quality in laying hens[J].Asian-Australas J Anim Sci, 2011, 24(10):1447-1455.
[63]	ALZAWQARI M H, KERMANSHAHI H, MOGHADDAM H N.The effect of glycine and desiccated ox bile supplementation on performance, fat digestibility, blood chemistry and ileal digesta viscosity of broiler chickens[J].Global Veterinaria, 2010, 5(3):1945-1953.
[64]	李亚杰, 刘禹辰, 曾丹, 等.添加不同水平丝氨酸对蛋鸡生产性能和蛋品质的影响[J].中国畜牧杂志, 2019, 55(2):86-89.LI Y J, LIU Y C, ZENG D, et al.Effects of adding different levels of serine on layer performance and egg quality[J].Chinese Journal of Animal Science, 2019, 55(2):86-89.(in Chinese)
[65]	陶红旭, 隋炳毅, 龙烁, 等.低蛋白日粮对蛋鸡生产性能和蛋品质的影响[J].中国家禽, 2014, 36(24):38-42.TAO H X, SUI B Y, LONG S, et al.Effect of low-protein diet on production performance and egg quality of laying hens[J].China Poultry, 2014, 36(24):38-42.(in Chinese)
[66]	ZHOU J M, QIU K, WANG J, et al.Effect of dietary serine supplementation on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed a low crude protein diet[J].Poult Sci, 2021, 100(12):101465.
[67]	DEAN D W, BIDNER T D, SOUTHERN L L.Glycine supplementation to low protein, amino acid-supplemented diets supports optimal performance of broiler chicks[J].Poult Sci, 2006, 85(2):288.
[68]	WANG Y Y, WANG Z H, SHAN Y Y.Assessment of the relationship between ovomucin and albumen quality of shell eggs during storage[J].Poult Sci, 2019, 98(1):473-479.
[69]	王晓翠, 武书庚, 岳洪源, 等.卵黏蛋白:结构组成、理化性质、在浓蛋白液化中的作用及营养调控[J].动物营养学报, 2015, 27(2):327-333.WANG X C, WU S G, YUE H Y, et al.Ovomucin:structure composition, physicochemical properties the role in egg white thinning and nutritional modulation[J].Chinese Journal of Animal Nutrition, 2015, 27(2):327-333.(in Chinese)
[70]	YUAN C, BU X C, YAN H X, et al.Dietary L-arginine levels affect the liver protein turnover and alter the expression of genes related to protein synthesis and proteolysis of laying hens[J].Poult Sci, 2016, 95(2):261-267.
[71]	袁超.精氨酸对蛋鸡采食及组织蛋白质代谢调控的机理研究[D].杭州:浙江大学, 2016.YUAN C.Research on the mechanism of l-arginine on the regulation of feed intake and tissue protein metabolism in laying hens[D].Hangzhou:Zhejiang University, 2016.(in Chinese)
[72]	李君.蛋鸡低蛋白日粮应用合成氨基酸的制约因素研究[D].泰安:山东农业大学, 2017.LI J.A study of constraints on the use of crystalline amino acids in low-protein diets for laying hens[D].Tai'an:Shandong Agricultural University, 2017.(in Chinese)
[73]	WANG X C, ZHANG H J, WANG H, et al.Effect of different protein ingredients on performance, egg quality, organ health, and jejunum morphology of laying hens[J].Poult Sci, 2017, 96(5):1316-1324.
[74]	HE T, ZHANG H J, WANG J, et al.Application of low-gossypol cottonseed meal in laying hens' diet[J].Poult Sci, 2015, 94(10):2456-2463.
[75]	何涛.脱酚棉籽蛋白对鸡蛋品质的影响及其机理[D].北京:中国农业科学院, 2016.HE T.Study on the application of low-gossypol cottonseed meal in laving hen diets[D].Beijing:Chinese Academy of Agricultural Sciences, 2016.(in Chinese)
[76]	WANG X C, ZHANG H J, WANG H, et al.Effect of dietary protein sources on production performance, egg quality, and plasma parameters of laying hens[J].Asian-Australas J Anim Sci, 2017, 30(3):400-409.
[77]	王晓翠.理想蛋白模式下饲粮蛋白源对蛋品质的影响及其机理研究[D].哈尔滨:东北农业大学, 2015.WANG X C.Effects of dietary protein sources on egg quality and its mechanism under ideal protein mode[D].Harbin:Northeast Agricultural University, 2015.(in Chinese)
[78]	KOÇER B, BOZKURT M, EGE G, et al.Effects of sunflower meal supplementation in the diet on productive performance, egg quality and gastrointestinal tract traits of laying hens[J].Br Poultry Sci, 2020, 62(1):101-109.
[79]	王娇, 李军国, 谷旭, 等.膨化大豆代替豆粕对鸡蛋营养指标和蛋品质的影响[J].饲料工业, 2019, 40(3):39-43.WANG J, LI J G, GU X, et al.Effects of extruded soybean substitution for soybean meal on nutritional indicator of eggs and egg quality of laying hens[J].Feed Industry, 2019, 40(3):39-43.(in Chinese)
[80]	SECCI G, BOVERA F, NIZZA S, et al.Quality of eggs from Lohmann Brown Classic laying hens fed black soldier fly meal as substitute for soya bean[J].Animal, 2018, 12(10):2191-2197.
[81]	MARONO S, LOPONTE R, LOMBARDI P, et al.Productive performance and blood profiles of laying hens fed Hermetia illucens larvae meal as total replacement of soybean meal from 24 to 45 weeks of age[J].Poult Sci, 2017, 96(6):1783-1790.
[82]	宋宇琨, 何俊.4种动物性蛋白质饲料的营养特性及其在畜禽养殖中的应用[J].动物营养学报, 2019, 31(1):109-118.SONG Y K, HE J.Nutritional characteristics of four animal protein feeds and their application in livestock and poultry breeding[J].Chinese Journal of Animal Nutrition, 2019, 31(1):109-118.(in Chinese)
[83]	车彦卓, 黄振吾, 武书庚, 等.不同饲粮粗蛋白质水平下黑水虻蛋白替代豆粕对蛋鸡生产性能、蛋清品质及血清蛋白质代谢指标的影响[J].动物营养学报, 2020, 32(4):1632-1640.CHE Y Z, HUANG Z W, WU S G, et al.Effects of soybean meal replaced by Hermitia illucens L. protein in different dietary crude protein levels on performance, albumen quality and serum proteometabolism indexes of laying hens[J].Chinese Journal of Animal Nutrition, 2020, 32(4):1632-1640.(in Chinese)
[84]	车彦卓, 宣秋希, 武书庚, 等.不同饲粮粗蛋白质水平下蝇蛆蛋白替代豆粕对蛋鸡生产性能、蛋清品质及血清蛋白质代谢指标的影响[J].动物营养学报, 2020, 32(4):1624-1631.CHE Y Z, XUAN Q X, WU S G, et al.Effects of soybean meal replaced by fly maggot protein in different dietary crude protein levels on performance, albumen quality and serum proteometabolism indexes of laying hens[J].Chinese Journal of Animal Nutrition, 2020, 32(4):1624-1631.(in Chinese)
[85]	张京和, 杨久仙, 彭晓培, 等.黄粉虫对蛋鸡产蛋性能和蛋品质影响的研究[J].饲料研究, 2013(6):62-64.ZHANG J H, YANG J X, PENG X P, et al.The effect of Tenebrio molitor on laying performance and egg quality of laying hens[J].Feed Research, 2013(6):62-64.(in Chinese)
[86]	LOLLI S, GRILLI G, FERRARI L, et al.Effect of different percentage of Camelina sativa cake in laying hens diet:Performance, welfare, and eggshell quality[J].Animals, 2020, 10(8):1396.
[87]	张琦.蛋鸡输卵管卵清蛋白表达的研究[D].泰安:山东农业大学, 2015.ZHANG Q.A study on the expression of ovalbumin in the oviduct magnum of laying hens[D].Tai'an:Shandong Agricultural University, 2017.(in Chinese)
[88]	KESHAVARZ K, AUSTIC R E.The Use of Low-protein, low-phosphorus, amino acid- and phytase-supplemented diets on laying hen performance and nitrogen and phosphorus excretion[J].Poult Sci, 2004, 83(1):75-83.
[89]	BURLEY H K, PATTERSON P H, ELLIOT M A.Effect of a reduced crude protein, amino acid-balanced diet on hen performance, production costs, and ammonia emissions in a commercial laying hen flock[J].J Appl Poultry Res, 2013, 22(2):217-228.
[90]	JI F, FU S Y, REN B, et al.Evaluation of amino-acid supplemented diets varying in protein levels for laying hens[J].J Appl Poult Res, 2014, 23(3):384-392.
[91]	KESHAVARZ K, JACKSON M E.Performance of growing pullets and laying hens fed low-protein, amino acid-supplemented diets[J].Poult Sci, 1992, 71(5):905-918.
[92]	孟艳莉, 白修云, 张甦寅, 等.低蛋白质平衡氨基酸日粮对蛋鸡生产性能、蛋品质和氮排泄的影响[J].中国饲料, 2014(10):20-24.MENG Y L, BAI X Y, ZHANG S Y, et al.Effects of low protein diets balanced with amino acids on laying performance, egg quality and nitrogen excretion in laying hens[J].China Feed, 2014(10):20-24.(in Chinese)
[93]	KHAJALI F, KHOSHOUIE E A, DEHKORDI S K, et al.Production performance and egg quality of Hy-line w36 laying hens fed reduced-protein diets at a constant total sulfur amino acid:lysine ratio[J].J Appl Poult Res, 2008, 17(3):390-397.
[94]	李永洙.氨基酸对蛋鸡生产性能及盲肠微生物菌群结构的影响[J].中国农业大学学报, 2012, 17(2):108-116.LI Y Z.Effect of amino acids supplementation in low-protein feed on the performance and cecal microflora structure of laying hens[J].Journal of China Agricultural University, 2012, 17(2):108-116.(in Chinese)
[95]	BEZERRA R M, COSTA F G P, GIVISIEZ P E N, et al.Glutamic acid supplementation on low protein diets for laying hens[J].Acta Sci, 2015, 37(2):129.