芦荟大黄素对衰老蛋鸡卵泡氧化应激的缓解作用

doi:10.11843/j.issn.0366-6964.2022.06.031

摘要/Abstract

摘要： 旨在提高衰老蛋鸡的产蛋量，蛋鸡腹腔注射10 mg·kg^-1芦荟大黄素，每天1次，连续7 d，研究天然的芦荟大黄素对580日龄(D580)衰老蛋鸡卵巢重量、卵泡数量和体重变化的影响。本试验建立D-gal诱导的衰老卵泡模型，探究芦荟大黄素对卵泡衰老过程中抗氧化能力、细胞凋亡和增殖的影响，然后检测芦荟大黄素对体外衰老卵泡模型中Nrf2/HO-1信号通路相关蛋白和基因表达水平的影响，以及Nrf2激活剂和阻断剂对芦荟大黄素引起的衰老卵泡氧化应激缓解作用的变化。结果表明，芦荟大黄素处理可提高各级卵泡的数量(P < 0.05)，其中，大黄卵泡、小黄卵泡、大白卵泡和小白卵泡数量分别提高32.00%、34.62%、50.00%和54.21%。同时，芦荟大黄素处理使D580蛋鸡血清中雌二醇(E₂)和孕酮(P₄)水平分别提高34.14%和680.00%。芦荟大黄素处理还可提高卵泡中雌激素受体(ERα和ERβ)和肝中甘油三酯(triglyceride, TG)的含量(P < 0.05)。D580蛋鸡肝组织中脂肪酸合成相关基因的转录水平显著下调，但芦荟大黄素处理可提升这些基因的转录水平。此外，芦荟大黄素通过提高卵泡抗氧化酶的活性、Nrf2/HO-1信号通路相关蛋白和基因的表达，缓解自然衰老和D-gal诱导的衰老卵泡的氧化应激。芦荟大黄素处理可促进衰老卵泡细胞增殖，抑制其凋亡。综上表明，芦荟大黄素通过激活Nrf2/HO-1信号通路缓解蛋鸡卵泡的氧化应激，提升蛋鸡衰老过程中卵泡雌激素受体基因和肝卵黄生成相关基因的表达量、血清E₂和P₄水平、卵泡抗氧化能力以及肝TG合成能力，从而缓解卵泡衰老。

关键词: 芦荟大黄素, 蛋鸡, 卵泡, 氧化应激

Abstract: To improve the egg production of aging laying hens, we administered intraperitoneal injections of 10 mg·kg^-1 aloe-emodin (AE) to laying hens once a day for 7 days. The effects of natural AE on ovarian weight, follicle number and body weight of 580 day old laying hens were studied. Then, detect the effects of AE on the expression level of Nrf2/HO-1 pathway related proteins and genes in D-gal-induced aging ovary, and the changes of AE in alleviating D-gal-induced aging ovary after adding Nrf2 activator and inhibitor, so as to determine whether the alleviating effect of AE on D-gal-induced aging ovary depends on Nrf2/HO-1 pathway. The results showed that AE treatment increased the number of follicles at all levels (P < 0.05), including 32.00%, 34.62%, 50.00% and 54.21% for large, small and large follicles, respectively. Besides, with the treatment of AE, the estradiol (E₂) and progesterone (P₄) levels in the serum of aging laying hens were elevated by 34.14% and 680.00%, respectively. Moreover, the AE treatment can also elevated estrogen receptors (ERs, ERα and ERβ)in follicles and TG content in liver (P < 0.05). Although the transcript levels of fatty acid synthesis related genes were significantly downregulated in D580 laying hens liver tissue, transcription levels of these genes can be elevated by AE treatment. Firstly, the putative attenuating effects, cell proliferation and apoptosis of AE (10 μg·mL^-1) was evaluate through the establishment of a 2.5 mg·mL^-1 D-gal-induced aging follicular model in vitro. The results showed that AE alleviated the D-gal-induced aging ovary and natural aging ovary by increasing the activity of antioxidant enzymes and raising the expression level of Nrf2/HO-1 pathway related proteins and genes. At the same time, AE enhanced the proliferation of senescent follicle cells and inhibited cell apoptosis. In summary, AE alleviated oxidavtive stress in aging hens by activating Nrf2/HO-1 pathway. Meanwhile, AE improved ERs in follicles and vitellogenesis related genes in liver, E₂ and P₄ levels in serum, follicle antioxidative ability and liver TC synthetic ability, so as to alleviate follicular aging.

Key words: aloe-emodin, chicken, ovary follicle, oxidative stress

中图分类号:

S853.74

胡哲炜, 谢储康, 陈艳琼, 周烁, 张才乔. 芦荟大黄素对衰老蛋鸡卵泡氧化应激的缓解作用[J]. 畜牧兽医学报, 2022, 53(6): 1980-1993.

HU Zhewei, XIE Chukang, CHEN Yanqiong, ZHOU Shuo, ZHANG Caiqiao. Alleviating Effect of Aloe-emodin on Follicle Oxidative Stress in Aging Hens[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1980-1993.

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