畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (2): 339-343.doi: 10.11843/j.issn.0366-6964.2015.02.023

• 研究简报 • 上一篇    下一篇

一株牦牛源御成门沙门菌对小鼠的致病性

雷占东,张斌,岳华,黄志宏,汤承*   

  1. (西南民族大学生命科学与技术学院,成都 610041)
  • 收稿日期:2014-06-03 出版日期:2015-02-23 发布日期:2015-02-23
  • 通讯作者: 汤承,E-mail:tangcheng101@163.com
  • 作者简介:雷占东(1987-),男,河南开封人,硕士生,主要从事兽医临床微生物研究,E-mail:leizhandong2008@163.com
  • 基金资助:

    “十二五”国家科技支撑计划课题(2012BAD13B06);四川省教育厅创新团队项目(13TD0057);西南民族大学中央高校基本科研业务费专项资金项目(2014NZYQN42;2014ZYXS57);西南民族大学研究生学位点建设项目(2014XWD-S0906)

The Study on Pathogenicity of a Yak Salmonella onarimon Isolates in Mice

LEI Zhan-dong,ZHANG Bin,YUE Hua,HUANG Zhi-hong,TANG Cheng*   

  1. (College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China)
  • Received:2014-06-03 Online:2015-02-23 Published:2015-02-23

摘要:

御成门沙门菌是沙门菌中的稀少血清型,其致病性尚未见报道。以BALB/c小鼠为模型,旨在研究牦牛源御成门沙门菌swun3736菌株的致病性。采用灌胃的方法,测定swun3736菌株对小鼠的半数致死量(LD50)、细菌在小鼠体内的动态分布及各组织的病理学变化等。结果表明,御成门沙门菌swun3736菌株对小鼠的LD50为5×102 CFU。以105 CFU•只-1灌胃感染小鼠,24 h后最先在肺中分离到该菌,72 h从脾、肝、肾及胰腺等器官中分离到该菌,96 h从心和大脑中分离到该菌。组织病理学检查显示肺、脾、肝、心、肾和回肠出现变性、坏死与炎性细胞浸润等病变。除回肠外,其他肠段未见明显病变,提示回肠为该菌的入侵门户。本试验结果表明御成门沙门菌swun3736具有很强的致病力,其公共卫生学意义值得关注。

Abstract:

Salmonella onarimon is a rare serotypes in Salmonella,and its pathogenicity has not been reported.The aim of this experiment was to study the pathogenicity of S.onarimon strain swun3736 isolated from yak in BALB/c mice.Using oral method,we studied its pathogenicity by the median lethal dose (LD50),the dynamic distribution and histopathological changes in tissues of mice.The results showed that the LD50 was 5 × 102 CFU.When infected with 105 CFU per mice,bacteria were first isolated from lungs at 24 h PI,and spleen,liver,kidneys and pancreas at 72 h post infection (PI),while brain and heart at 96 h PI.In histopathological examination,it appeared the degeneration,necrosis,inflammatory cell infiltration and other serious damage in the lung,spleen,liver,heart,kidney and ileum.While excepting for ileum,other intestinal bowels exhibited no obvious lesions,suggesting that the ileum was the invasion portal of swun3736.These results demonstrated that S.onarimon swun3736 has strongly virulence,which indicated that public hygienics significance was worth concerned.

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