畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (2): 344-348.doi: 10.11843/j.issn.0366-6964.2015.02.024

• 研究简报 • 上一篇    

山羊源副流感病毒3型的分离与分子鉴定

李文良1,毛立1,程素平2,王秋生2,黄加春3,张纹纹1,杨蕾蕾1,江杰元1*   

  1. (1.江苏省农业科学院兽医研究所,农业部兽用生物制品工程技术重点实验室,国家兽用生物制品工程技术研究中心,南京 210014;2.海安县畜牧兽医站,海安 226600;3.南通点石生物科技有限公司,南通 226000)
  • 收稿日期:2014-06-18 出版日期:2015-02-23 发布日期:2015-02-23
  • 通讯作者: 江杰元,研究员,博士,Tel:025-84391135,E-mail:jieyuanj57@gmail.com
  • 作者简介:李文良(1984-),男,河南开封人,副研究员,博士,主要从事动物传染病防治和诊断技术研究,E-mail:kfliwenliang@163.com
  • 基金资助:

    江苏省农业科技自主创新资金项目(CX(14)2090)

Isolation and Identification of Caprine Derived Parainfluenza Virus Type 3

LI Wen-liang1,MAO Li1,CHENG Su-ping2,WANG Qiu-sheng2,HUANG Jia-chun3,ZHANG Wen-wen1,YANG Lei-lei1,JIANG Jie-yuan1*   

  1. (1.Key Laboratory of Veterinary Biological Engineering and Technology of Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products,Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.Hai’an animal husbandry and veterinary station,Hai’an 226600,China;3.Dianshi biological technology Co.,Ltd.,Nantong 226000,China)
  • Received:2014-06-18 Online:2015-02-23 Published:2015-02-23

摘要:

2013年下半年至2014年3月,江苏多地育肥羊出现呼吸道症状为主的疾病。采集不同发病场鼻拭子和血清样品,检测呼吸道相关病原,在大部分样品中检测到副流感病毒3型。将阳性病料接种于MDBK细胞,连续传代5代,经RT-PCR和血凝试验检测证实分离到病毒,命名为JS2013。扩增完整的M基因,进行进化分析表明该毒株不同于牛和人副流感病毒3型,具有独特的基因特征。这是国内外首次报道山羊源副流感病毒3型的分离鉴定。

Abstract:

From Aug 2013 to Mar 2014,fattened goat herds of many farms in Jiangsu province suffered severe respiratory disease.Serum and nasal swab samples were collected and subjected to pathogen detection.Parainfluenza virus type 3 (PIV3) was detected in most of the samples.Positive samples were inoculated onto Madin-Darby bovine kidney (MDBK) cells and passaged 5 times for virus isolation.A novel strain,named JS2013,was successfully isolated as identified by RT-PCR and hemagglutination test.Entire M coding region was amplified and sequenced.Phylogenetic analysis and comparison to other reference sequences available in GenBank indicated JS2013 was clearly distinct from other HPIV3 and BPIV3 strains.This is the first isolation and partial genetic identification of PIV3 from diseased goats.

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