β-内酰胺类药物对副猪嗜血杆菌流行病学临界值的建立及耐药性的测定

doi:10.11843/j.issn.0366-6964.2021.011.025

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (11): 3234-3245.doi: 10.11843/j.issn.0366-6964.2021.011.025

β-内酰胺类药物对副猪嗜血杆菌流行病学临界值的建立及耐药性的测定

陈超群, 陈佳莉, 周萱仪, 吴雪, 汪津丞, 黄安雄, 郝海红*

华中农业大学国家兽药残留基准实验室, 农业部兽药残留检测重点实验室, 国家兽药安全评价实验室, 农业部畜禽产品质量安全风险评估实验室(武汉), 武汉 430070

收稿日期:2021-02-01 出版日期:2021-11-23 发布日期:2021-11-24
通讯作者: 郝海红,主要从事细菌耐药性和兽药合理应用研究,E-mail:haohaihong@mail.hzau.edu.cn
作者简介:陈超群(1999-),女,安徽合肥人,主要从事细菌耐药性和兽药合理应用研究,E-mail:2078532896@qq.com
基金资助:
国家重点研究发展计划（2016YFD0501302/2017YFD0501406）；国家自然科学基金（31772791）；中央高校自主创新基金（2662018 JC001）；华中农业大学SRF项目（2019058）；湖北省大学生创新创业计划（S201910504008）

Establishment of Epidemiological Cut-off Values and Determination of Drug Resistance of Haemophilus parasuis with β-lactam Drugs

CHEN Chaoqun, CHEN Jiali, ZHOU Xuanyi, WU Xue, WANG Jincheng, HUANG Anxiong, HAO Haihong*

National Reference Laboratory of Veterinary Drug Residues, The Key Laboratory for the Detection of Veterinary Drug Residues of Ministry of Agriculture, National Laboratory for Safety Evaluation of Veterinary Drugs, Huazhong Agricultural University, Laboratory of Quality & Safety Risk Assessment for Livestock and Poultry Product s(Wuhan), Wuhan 430070, China

Received:2021-02-01 Online:2021-11-23 Published:2021-11-24

摘要/Abstract

摘要： 副猪嗜血杆菌病是副猪嗜血杆菌主要针对保育仔猪的一种全身多发性的传染病，对猪场的危害极大。β-内酰胺类抗生素是临床上使用比较多的一类抗生素，具有杀菌活性强、毒性低、适应症广及临床疗效好等优点，常用于治疗副猪嗜血杆菌病，因此有必要了解副猪嗜血杆菌对β-内酰胺类抗生素的耐药情况，从而更科学地指导临床用药和新药开发。本研究参考CLSI-VET和VETCAST中流行病学临界值的建立方法，汇总不同地区来源的菌株对14种β-内酰胺类药物的药敏结果，建立β-内酰胺类药物的流行病学临界值。结果显示，头孢克洛、头孢吡肟、头孢噻肟、头孢喹肟、头孢噻呋、头孢氨苄、阿莫西林-克拉维酸、阿莫西林、克拉维酸、氨苄西林、青霉素、苯唑西林、亚胺培南和美罗培南的流行病学折点值分别为16、0.5、0.125、0.031 25、0.5、32、0.25、1、0.5、1、2、8、0.25、0.062 5 μg·mL^-1，可以得出副猪嗜血杆菌对氨苄西林、阿莫西林以及头孢吡肟的耐药率较高，对亚胺培南和阿莫西林-克拉维酸的敏感性较高。在CLSI （Clinical and Laboratory Standards Institute）和EUCAST （The European Committee on Antimicrobial Susceptibility Testing）缺乏敏感性评判标准的情况下，本研究可以直观地识别非野生型菌株的出现，有利于耐药性监测工作的开展，对副猪嗜血杆菌的治疗和防控具有一定的参考价值。

关键词: 副猪嗜血杆菌, β-内酰胺类药物, 流行病学临界值, 耐药性

Abstract: Haemophilus parasuis disease is a systemic infection of swine caused by Haemophilus parasuis. It mainly affects piglets at the nursery stage and is extremely harmful to pig farms. β-lactam drug is a kind of antibiotics used more clinically, and has the advantages of strong bactericidal activity, low toxicity, wide indications and good clinical curative effect, also often used to treat Haemophilus parasuis. To understand the drug resistance of Haemophilus parasuis to β-lactam antibiotics, and guide clinical drug use and new drug development more scientifically, this study referred to the method of establishing the epidemiological cut-off values in CLSI, to summarize the susceptibility results of strains from different regions to β-lactam drugs (determined by broth dilution method)， and to establish ECOFFs for β-lactam drugs. The ECOFFs of cefaclor，cefepime, cefotaxime, cefoquinme, ceftiofur, cefalexin, amoxicillin-clavulanic acid, amoxicillin, clavulanic acid, ampicillin, penicillin, oxacillin, imipenem and meropenem were recommended to be 16, 0.5, 0.125, 0.031 25, 0.5, 32， 0.25， 1， 0.5， 1， 2， 8， 0.25， 0.062 5 μg·mL^-1. In the absence of CLSI and EUCAST sensitivity criteria, the appearance of non-wild-type strains can be recognized directly, which is beneficial to the development of drug resistance monitoring and has certain reference value for the treatment and control of Haemophilus parasuis.

Key words: Haemophilus parasuis, β-lactam drugs, epidemiological cut-off values, drug-resistance

中图分类号:

陈超群, 陈佳莉, 周萱仪, 吴雪, 汪津丞, 黄安雄, 郝海红. β-内酰胺类药物对副猪嗜血杆菌流行病学临界值的建立及耐药性的测定[J]. 畜牧兽医学报, 2021, 52(11): 3234-3245.

CHEN Chaoqun, CHEN Jiali, ZHOU Xuanyi, WU Xue, WANG Jincheng, HUANG Anxiong, HAO Haihong. Establishment of Epidemiological Cut-off Values and Determination of Drug Resistance of Haemophilus parasuis with β-lactam Drugs[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3234-3245.

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β-内酰胺类药物对副猪嗜血杆菌流行病学临界值的建立及耐药性的测定

Establishment of Epidemiological Cut-off Values and Determination of Drug Resistance of Haemophilus parasuis with β-lactam Drugs

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