Abstract:

This experiment was conducted to develop the infection model of Mycoplasma hyopneumoniae in vitro and study the adhesion characteristics by indirect immunofluorescence method in different cell lines. Several Mhp strains which include field strains (XLW-1, XLW-2, XLW-3, WX), international standard strain 232 and the attenuated strain (168) were selected, and infected Pig Kidney15 （PK15）. The results showed that all the Mhp whole bacteria strains could adhere to PK15 cell lines with different adhesion efficiency. And the order of adhesion ability of Mhp was field strain XLW-1, WX, the attenuated strain (168), international standard strain 232, field strain XLW-2, XLW-3. The field strain XLW-1 had the best adhesion ability, so it was selected as test strain to study the adhesion ability to other cell lines, such as the original generated cells of pig tracheal epithelial, porcine alveolar epithelial cells (SJPL), porcine alveolar macrophages (3D4/31) and rhesus monkey kidney cells (Marc145). XLW-1 strains can adhere to different cell lines in vitro, but the different adhesion capacity also showed on the different cell lines. XLW-1 strain has good adhesion ability to pig tracheal epithelial cells in primary separation, the SJPL cell lines and 3D4/31 cell lines, while very weak ability to the non-swine cell lines, Marc145 cell line. In conclusion, Mycoplasma hyopneumoniae adhesion model was developed on different cell lines, and different Mhp strains showed different adhesion ability on the same cell lines, as well as the same strain showed the different adhesion on different cell lines.