动物支原体相关蛋白的免疫原性研究进展

doi:10.11843/j.issn.0366-6964.2021.05.009

摘要/Abstract

摘要： 动物支原体可引起肺炎、关节炎、乳腺炎等一系列重要疾病，临床上常表现为慢性、持续性感染，给养殖业造成严重的经济损失。亚单位疫苗具有安全高效、成本低廉等优点，是支原体疫苗研究的重要发展方向。本文主要介绍了重要动物支原体相关蛋白的免疫原性研究进展，以期为今后动物支原体病亚单位疫苗研究提供参考。

关键词: 动物支原体, 支原体, 蛋白, 免疫原性, 亚单位疫苗, 免疫蛋白

Abstract: Animal mycoplasmas contribute to some important diseases including pneumonia, mastitis, and arthritis, characterized by chronic and persistent infection, which causes serious economic losses to the breeding industry. Subunit vaccine has the advantages of safety, high efficiency, low cost, etc. It is an important direction of mycoplasma vaccine research. This paper describes the research advances in immunogenicity of related proteins of important animal mycoplasmas. It would be helpful to provide references for research of subunit vaccine of animal mycoplasmas.

Key words: animal mycoplasmas, mycoplasma, protein, immunogenicity, subunit vaccine, immune proteins

中图分类号:

S852.62

陈胜利, 郝华芳, 季文恒, 储岳峰. 动物支原体相关蛋白的免疫原性研究进展[J]. 畜牧兽医学报, 2021, 52(5): 1230-1237.

CHEN Shengli, HAO Huafang, JI Wenheng, CHU Yuefeng. Research Advances in Immunogenicity of Related Proteins of Animal Mycoplasmas[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1230-1237.

参考文献 59

[1]	吴移谋, 叶元康. 支原体学[M]. 2版. 北京:人民卫生出版社, 2008.WU Y M，YE Y K. Mycoplasma[M]. 2nd ed. Beijing: People’s Medical Publishing Press, 2008.(in Chinese)
[2]	World Organization for Animal Health. Chapter 3.7.4 Contagious caprine pleuropneumonia [EB/OL] //Manual of diagnostic tests and vaccines for terrestrial animals 2018. [2020-06-05]. https://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/3.07.04_CCPP.pdf.
[3]	KLEIN U, DE JONG A, YOUALA M, et al. New antimicrobial susceptibility data from monitoring of Mycoplasma bovis isolated in Europe[J]. Vet Microbiol, 2019, 238:108432.
[4]	CAI H Y, MCDOWALL R, PARKER L, et al. Changes in antimicrobial susceptibility profiles of Mycoplasma bovis over time[J]. Can J Vet Res, 2019, 83(1):34-41.
[5]	ABD EL-HAMID M I, AWAD N F S, HASHEM Y M, et al. In vitro evaluation of various antimicrobials against field Mycoplasma gallisepticum and Mycoplasma synoviae isolates in Egypt[J]. Poult Sci, 2019, 98(12):6281-6288.
[6]	GAUTIER-BOUCHARDON A V. Antimicrobial resistance in Mycoplasma spp. [J]. Microbiol Spectr, 2018, 6(4), doi:10. 1128/microbiolspec. ARBA-0030-2018.
[7]	KLEIN U, DE JONG A, MOYAERT H, et al. Antimicrobial susceptibility monitoring of Mycoplasma hyopneumoniae and Mycoplasma bovis isolated in Europe[J]. Vet Microbiol, 2017, 204:188-193.
[8]	TAO Y, SHU J H, CHEN J, et al. A concise review of vaccines against Mycoplasma hyopneumoniae[J]. Res Vet Sci, 2019, 123:144-152.
[9]	CALCUTT M J, LYSNYANSKY I, SACHSE K, et al. Gap analysis of Mycoplasma bovis disease, diagnosis and control:an aid to identify future development requirements[J]. Transbound Emerg Dis, 2018, 65(S1):91-109.
[10]	MAES D, SIBILA M, KUHNERT P, et al. Update on Mycoplasma hyopneumoniae infections in pigs:knowledge gaps for improved disease control[J]. Transbound Emerg Dis, 2018, 65(S1):110-124.
[11]	WELDEAREGAY Y B, PICH A, SCHIECK E, et al. Proteomic characterization of pleural effusion, a specific host niche of Mycoplasma mycoides subsp.mycoides from cattle with contagious bovine pleuropneumonia (CBPP)[J]. J Proteomics, 2016, 131:93-103.
[12]	KRASTEVA I, LILJANDER A, FISCHER A, et al. Characterization of the in vitro core surface proteome of Mycoplasma mycoides subsp.mycoides, the causative agent of contagious bovine pleuropneumonia[J]. Vet Microbiol, 2014, 168(1):116-123.
[13]	MULONGO M M, FREY J, SMITH K, et al. Cattle immunized against the pathogenic L-α-glycerol-3-phosphate oxidase of Mycoplasma mycoides subs.mycoides fail to generate neutralizing antibodies and succumb to disease on challenge[J]. Vaccine, 2013, 31(44):5020-5025.
[14]	MULONGO M, FREY J, SMITH K, et al. Vaccination of cattle with the N terminus of LppQ of Mycoplasma mycoides subsp.mycoides results in type III immune complex disease upon experimental infection[J]. Infect Immun, 2015, 83(5):1992-2000.
[15]	DEDIEU L, TOTTE P, RODRIGUES V, et al. Comparative analysis of four lipoproteins from Mycoplasma mycoides subsp.mycoides small colony identifies LppA as a major T-cell antigen[J]. Comp Immunol Microbiol Infect Dis, 2010, 33(4):279-290.
[16]	MWIRIGI M, NKANDO I, OLUM M, et al. Capsular polysaccharide from Mycoplasma mycoides subsp.mycoides shows potential for protection against contagious bovine pleuropneumonia[J]. Vet Immunol Immunopathol, 2016, 178:64-69.
[17]	PEREZ-CASAL J, PRYSLIAK T, MAINA T, et al. Analysis of immune responses to recombinant proteins from strains of Mycoplasma mycoides subsp.mycoides, the causative agent of contagious bovine pleuropneumonia[J]. Vet Immunol Immunopathol, 2015, 168(1-2):103-110.
[18]	NKANDO I, PEREZ-CASAL J, MWIRIGI M, et al. Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia[J]. Vet Immunol Immunopathol, 2016, 171:103-114.
[19]	ZUBAIR M, MUHAMED S A, KHAN F A, et al. Identification of 60 secreted proteins for Mycoplasma bovis with secretome assay[J]. Microb Pathog, 2020, 143:104135.
[20]	PRYSLIAK T, VAN DER MERWE J, PEREZ-CASAL J. Vaccination with recombinant Mycoplasma bovis GAPDH results in a strong humoral immune response but does not protect feedlot cattle from an experimental challenge with M.bovis[J]. Microb Pathog, 2013, 55:1-8.
[21]	MULONGO M, PRYSLIAK T, PEREZ-CASAL J. Vaccination of feedlot cattle with extracts and membrane fractions from two Mycoplasma bovis isolates results in strong humoral immune responses but does not protect against an experimental challenge[J]. Vaccine, 2013, 31(10):1406-1412.
[22]	PRYSLIAK T, PEREZ-CASAL J. Immune responses to Mycoplasma bovis proteins formulated with different adjuvants[J]. Can J Microbiol, 2016, 62(6):492-504.
[23]	SUN X L, JONES H P, HODGE L M, et al. Cytokine and chemokine transcription profile during Mycoplasma pulmonis infection in susceptible and resistant strains of mice:macrophage inflammatory protein 1β (CCL4) and monocyte chemoattractant protein 2(CCL8) and accumulation of CCR5+ Th cells[J]. Infect Immun, 2006, 74(10):5943-5954.
[24]	WU Q, MARTIN R J, RINO J G, et al. IL-23-dependent IL-17 production is essential in neutrophil recruitment and activity in mouse lung defense against respiratory Mycoplasma pneumoniae infection[J]. Microbes Infect, 2007, 9(1):78-86.
[25]	PRYSLIAK T, MAINA T, PEREZ-CASAL J. Th-17 cell mediated immune responses to Mycoplasma bovis proteins formulated with Montanide ISA61 VG and curdlan are not sufficient for protection against an experimental challenge with Mycoplasma bovis[J]. Vet Immunol Immunopathol, 2018, 197:7-14.
[26]	季文恒, 储岳峰, 赵萍, 等. 牛支原体逃避宿主免疫的研究进展[J]. 畜牧兽医学报, 2017, 48(3):393-402.JI W H, CHU Y F, ZHAO P, et al. Advances in the research of immune evasion by Mycoplasma bovis[J]. Acta Veterinaria et Zootechnica Sinica, 2017, 48(3):393-402. (in Chinese)
[27]	PEREZ-CASAL J. Pathogenesis and virulence of Mycoplasma bovis[J]. Vet Clin North Am Food Anim Pract, 2020, 36(2):269-278.
[28]	康浩然, 刘重阳, 于勇, 等. 2017-2018年我国部分地区牛支原体的分离鉴定及多位点序列分型[J]. 畜牧兽医学报, 2019, 50(9): 1857-1863.KANG H R, LIU C Y, YU Y, et al. Isolation, identification and multilocus sequence typing analysis of Mycoplasma bovis strains in some regions of China during 2017 to 2018[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(9): 1857-1863. (in Chinese)
[29]	CHEN S L, HAO H F, ZHAO P, et al. Differential immunoreactivity to bovine convalescent serum between Mycoplasma bovis biofilms and planktonic cells revealed by comparative immunoproteomic analysis[J]. Front Microbiol, 2018, 9:379.
[30]	ZHANG Y K, LI X, ZHAO H R, et al. Antibodies specific to membrane proteins are effective in complement-mediated killing of Mycoplasma bovis[J]. Infect Immun, 2019, 87(12):e00740-19.
[31]	刘茂军, 张悦, 杜改梅, 等. 猪肺炎支原体P65基因原核表达及免疫原性分析[J]. 畜牧兽医学报, 2014, 45(2):262-267.LIU M J, ZHANG Y, DU G M, et al. Prokaryotic expression and immunogenicity identification of P65 from Mycoplasma hyopneumoniae[J]. Acta Veterinaria et Zootechnica Sinica, 2014, 45(2):262-267. (in Chinese)
[32]	GALLI V, SIMIONATTO S, MARCHIORO S B, et al. Immunisation of mice with Mycoplasma hyopneumoniae antigens P37, P42, P46 and P95 delivered as recombinant subunit or DNA vaccines[J]. Vaccine, 2012, 31(1):135-140.
[33]	马丰英, 姚睿玉, 邹浩勇, 等. 猪支原体肺炎亚单位疫苗的免疫应答[J]. 中国兽医学报, 2012, 32(4):529-533.MA F Y, YAO R Y, ZOU H Y, et al. Development of the subunit vaccine against Mycoplasmal pneumonia of swine[J]. Chinese Journal of Veterinary Science, 2012, 32(4):529-533. (in Chinese)
[34]	CHEN C, YONG Q, JUN G, et al. Designing, expression and immunological characterization of a chimeric protein of Mycoplasma pneumoniae[J]. Protein Peptide Lett, 2016, 23(7):592-596.
[35]	DE OLIVEIRA N R, JORGE S, GOMES C K, et al. A novel chimeric protein composed of recombinant Mycoplasma hyopneumoniae antigens as a vaccine candidate evaluated in mice[J]. Vet Microbiol, 2017, 201:146-153.
[36]	BARATE A K, CHO Y, TRUONG Q L T, et al. Immunogenicity of IMS 1113 plus soluble subunit and chimeric proteins containing Mycoplasma hyopneumoniae P97 C-terminal repeat regions[J]. FEMS Microbiol Lett, 2014, 352(2):213-220.
[37]	WOOLLEY L K, FELL S A, GONSALVES J R, et al. Evaluation of recombinant Mycoplasma hyopneumoniae P97/P102 paralogs formulated with selected adjuvants as vaccines against mycoplasmal pneumonia in pigs[J]. Vaccine, 2014, 32(34):4333-4341.
[38]	JORGE S, DE OLIVEIRA N R, MARCHIORO S B, et al. The Mycoplasma hyopneumoniae recombinant heat shock protein P42 induces an immune response in pigs under field conditions[J]. Comp Immunol Microbiol Infect Dis, 2014, 37(4):229-236.
[39]	付启欢, 丁红雷, 吴建云. 猪支原体肺炎疫苗研究进展[J]. 中国兽医学报, 2016, 36(7):1251-1255.FU Q H, DING H L, WU J Y. Advance in research of Mycoplasma Hyopneumoniae vaccine[J]. Chinese Journal of Veterinary Science, 2016, 36(7):1251-1255.(in Chinese)
[40]	JAN G, LE HéNAFF M, FONTENELLE C, et al. Biochemical and antigenic characterisation of Mycoplasma gallisepticum membrane proteins P52 and P67(pMGA)[J]. Arch Microbiol, 2001, 177(1):81-90.
[41]	TAN L, HU M R, YU S Q, et al. Characterization of the chaperonin GroEL in Mycoplasma gallisepticum[J]. Arch Microbiol, 2015, 197(2):235-244.
[42]	MOURA L, DOHMS J, ALMEIDA J M, et al. Development and evaluation of a novel subunit vaccine for Mycoplasma gallisepticum[J]. Arq Bras Med Vet Zootec, 2012, 64(6):1569-1576.
[43]	CHEN S L, HAO H F, ZHAO P, et al. Genome-wide analysis of the first sequenced Mycoplasma capricolum subsp.capripneumoniae strain M1601[J]. G3(Bethesda), 2017, 7(9):2899-2906.
[44]	HAO H F, CHEN S L, LI Y G, et al. Complete genome sequence of Mycoplasma capricolum subsp.capripneumoniae Strain zly1309F, isolated from endangered Tibetan Antelope[J]. Genome Announc, 2017, 5(29):e00496-17.
[45]	LI Y, WANG R, SUN W J, et al. Comparative genomics analysis of Mycoplasma capricolum subsp.capripneumoniae 87001[J]. Genomics, 2020, 112(1):615-620.
[46]	ZHAO P, HE Y, CHU Y F, et al. Identification of novel immunogenic proteins in Mycoplasma capricolum subsp.capripneumoniae strain M1601[J]. J Vet Med Sci, 2012, 74(9):1109-1115.
[47]	YATOO M I, PARRAY O R, MUHEET, et al. Novel candidates for vaccine development against Mycoplasma capricolum subspecies Capripneumoniae (Mccp)-current knowledge and future prospects[J]. Vaccines, 2019, 7(3):71.
[48]	LILJANDER A, SACCHINI F, STOFFEL M H, et al. Reproduction of contagious caprine pleuropneumonia reveals the ability of convalescent sera to reduce hydrogen peroxide production in vitro[J]. Vet Res, 2019, 50(1):10.
[49]	SCHUMACHER M, NICHOLSON P, STOFFEL M H, et al. Evidence for the cytoplasmic localization of the L-α-Glycerophosphate oxidase in members of the “Mycoplasma mycoides Cluster”[J]. Front Microbiol, 2019, 10:1344.
[50]	赵萍, 陈胜利, 郝华芳, 等. 一种山羊支原体山羊肺炎亚种多重抗原肽的小鼠免疫试验[J]. 畜牧兽医学报, 2016, 47(12):2476-2482.ZHAO P, CHEN S L, HAO H F, et al. Immune responses in mice immunized with a recombinant multiple antigenic peptide from Mycoplasma capricolum subsp.capripneumoniae[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(12):2476-2482. (in Chinese)
[51]	夏业才, 陈光华, 丁家波. 兽医生物制品学[M]. 2版. 北京:中国农业出版社, 2018.XIA Y C, CHEN G H, DING J B. Science of veterinary biologicals[M]. 2nd ed. Beijing: China Agriculture Press, 2018. (in Chinese)
[52]	张悦, 刘茂军, 邵国青. 猪肺炎支原体主要抗原蛋白的研究进展[J]. 中国农学通报, 2013, 29(2):16-22.ZHANG Y, LIU M J, SHAO G Q. Advancement of major immunogenicity proteins on Mycoplasma hyopneumoniae[J]. Chinese Agricultural Science Bulletin, 2013, 29(2):16-22. (in Chinese)
[53]	王文秀, 王宝琴, 徐晴晴, 等. 猪肺炎支原体主要保护性抗原的研究进展[J]. 家畜生态学报, 2018, 39(6):79-85.WANG W X, WANG B Q, XU Q Q, et al. Research progress on major immunogenicity proteins on Mycoplasma hyopneumoniae[J]. Acta Ecologiae Animalis Domastici, 2018, 39(6):79-85. (in Chinese)
[54]	FAGAN P K, DJORDJEVIC S P, CHIN J, et al. Oral immunization of mice with attenuated Salmonella typhimurium aroA expressing a recombinant Mycoplasma hyopneumoniae antigen (NrdF)[J]. Infect Immun, 1997, 65(6):2502-2507.
[55]	OKAMBA F R, ARELLA M, MUSIC N, et al. Potential use of a recombinant replication-defective adenovirus vector carrying the C-terminal portion of the P97 adhesin protein as a vaccine against Mycoplasma hyopneumoniae in swine[J]. Vaccine, 2010, 28(30):4802-4809.
[56]	卢会英, 沈青春, 宁宜宝. 猪肺炎支原体p97 R1区基因和大肠杆菌LTB基因的重组和表达[J]. 中国兽医杂志, 2010, 46(4):3-6.LU H Y, SHEN Q C, NING Y B. The recombination and expression of R1 region of Mycoplasma hyopneumoniae p97 adhesin with Escherichia coli heat-labile enterotoxin B subunit[J]. Chinese Journal of Veterinary Medicine, 2010, 46(4):3-6. (in Chinese)
[57]	ISHAG H Z A, WU Y Z, LIU M J, et al. In vitro protective efficacy of Lithium chloride against Mycoplasma hyopneumoniae infection[J]. Res Vet Sci, 2016, 106:93-96.
[58]	VIRGINIO V G, BANDEIRA N C, DOS ANJOS LEAL F M, et al. Assessment of the adjuvant activity of mesoporous silica nanoparticles in recombinant Mycoplasma hyopneumoniae antigen vaccines[J]. Heliyon, 2017, 3(1):e00225.
[59]	陶宇, 李高建, 舒建洪, 等. 猪支原体肺炎基因工程疫苗的研究进展[J]. 中国生物工程杂志, 2018, 38(2):95-101.TAO Y, LI G J, SHU J H, et al. Advances in the research of genetically engineering vaccine of Mycoplasmal pneumoniae[J]. China Biotechnology, 2018, 38(2):95-101. (in Chinese)