玉米赤霉烯酮内酯水解酶的研究及其在畜禽日粮中的应用

doi:10.11843/j.issn.0366-6964.2025.10.007

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (10): 4839-4850.doi: 10.11843/j.issn.0366-6964.2025.10.007

玉米赤霉烯酮内酯水解酶的研究及其在畜禽日粮中的应用

俞良诚¹^,²(), 孙博¹, 任曼², 闫雪³, 刘宽博¹, 宋佳¹, 岳隆耀², 王薇薇¹^,*(), 赵晨¹^,*()

1. 国家粮食和物资储备局科学研究院国家粮食和物资储备局粮油生物技术重点实验室, 北京 100037
2. 安徽科技学院动物科学学院安徽省动物营养调控与健康重点实验室, 凤阳 233100
3. 新希望六和股份有限公司/畜禽饲料与畜禽产品质量安全控制四川省重点实验室, 成都 610023

收稿日期:2024-11-07 出版日期:2025-10-23 发布日期:2025-11-01
通讯作者: 王薇薇,赵晨 E-mail:yulc369@163.com;www@ags.ac.cn;zc@ags.ac.cn
作者简介:俞良诚(1999-), 男, 安徽宣城人, 硕士生, 主要从事粮油危害物生物降解研究, E-mail: yulc369@163.com
基金资助:
中央级公益性科研院所基本科研业务费项目(ZX2421)

The Research on Zearalenone Lactonase and Its Application in Livestock and Poultry Diets

YU Liangcheng¹^,²(), SUN Bo¹, REN Man², YAN Xue³, LIU Kuanbo¹, SONG Jia¹, YUE Longyao², WANG Weiwei¹^,*(), ZHAO Chen¹^,*()

1. Key Laboratory of Grain and Oil Biotechnology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, China
2. Anhui Provincial Key Laboratory of Animal Nutritional Regulation and Health, College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China
3. New Hope Liuhe Co., Ltd/Sichuan Key Laboratory of Feed and Livestock and Poultry Products Quality & Safety Control, Chengdu 610023, China

Received:2024-11-07 Online:2025-10-23 Published:2025-11-01
Contact: WANG Weiwei, ZHAO Chen E-mail:yulc369@163.com;www@ags.ac.cn;zc@ags.ac.cn

摘要/Abstract

摘要：

玉米赤霉烯酮(ZEN)及其衍生物是全球危害最严重的真菌毒素之一, 严重威胁粮食安全和人类健康。ZEN的生物降解技术开发一直是研究的热点。其中酶降解法因其特异性和有效性受到了广泛关注, ZEN降解酶主要包括漆酶、锰过氧化物酶和内酯水解酶。相较于前两类酶, 内酯水解酶因其高活性、降解机制清晰且降解产物无毒等优点, 使其具有ZEN脱毒酶制剂开发潜力。但天然酶在工业生产条件下的稳定性较差, 难以满足产品制备和实际应用的要求。通过理性或半理性设计等优化手段提升天然酶的活性和稳定性是目前的热点研究方向。本文综述了微生物源ZEN内酯水解酶的基因挖掘、特性表征、分子改造手段及其应用, 旨在阐明生物脱毒法在工业生产和畜牧业应用中的优势与挑战, 为开发高活性、高稳定性ZEN脱毒酶制剂提供新思路。

关键词: 玉米赤霉烯酮, 内酯水解酶, 理性设计, 酶活稳定性, 稳定性优化, 毒素降解

Abstract:

Zearalenone (ZEN) and its derivatives rank among the most hazardous mycotoxins worldwide, posing a significant threat to food safety and public health. Consequently, the development of efficient biodegradation strategies for ZEN has emerged as a critical research priority. Among these, enzymatic degradation approaches have garnered considerable attention due to their specificity and efficacy.ZEN-degrading enzymes primarily include laccases, manganese peroxidases, and lactonases. Compared to the first two enzyme classes, lactonases exhibit distinct advantages for the development of ZEN detoxification agents, including higher catalytic activity, well-defined degradation mechanisms, and non-toxic degradation byproducts. However, natural enzymes often suffer from limited stability under industrial processing conditions, hindering their applicability in large-scale production and practical settings. To address this challenge, enhancing enzyme activity and stability through rational or semi-rational design and optimization has become a key research focus.This review comprehensively examines microbial-derived ZEN lactonases, covering gene mining, biochemical characterization, molecular engineering, and practical applications. By evaluating the advantages and limitations of biological detoxification in industrial and livestock production, this analysis provides novel insights for the development of highly active, robust ZEN-detoxifying enzyme preparations.

Key words: zearalenone, lactonase, rational design, enzyme activity stability, stability optimization, toxin degradation

中图分类号:

S859.8

俞良诚, 孙博, 任曼, 闫雪, 刘宽博, 宋佳, 岳隆耀, 王薇薇, 赵晨. 玉米赤霉烯酮内酯水解酶的研究及其在畜禽日粮中的应用[J]. 畜牧兽医学报, 2025, 56(10): 4839-4850.

YU Liangcheng, SUN Bo, REN Man, YAN Xue, LIU Kuanbo, SONG Jia, YUE Longyao, WANG Weiwei, ZHAO Chen. The Research on Zearalenone Lactonase and Its Application in Livestock and Poultry Diets[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(10): 4839-4850.

图/表 3

图 1

图 2

表 1

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名称(PDB代码) Name(PDB code)	来源生物Source organism	底物Substrate	最适pH/温度(℃) Optimal pH/ temperature(℃)	比活/(U·mg^-1) Specific activities	参考文献References
ZHD101 (BAC02717)	C. rosea	ZEN、α/β-ZOL、α/β-ZAL、ZAN	10.5/37~45	NR	[11]
ZenH (WP_269305865.1)	Aeromicrobium sp. HA	ZEN	7.0/55	7.05	[17]
ZENM (RYP08044.1)	Monosporascus sp. GIB2	ZEN、α-ZOL、α-ZAL	9.0/60	333	[18]
ZenC (XM_958243.3)	Neurospora crassa	ZEN	8.0/45	530.4	[19]
ZHD518 (XM_013418296)	NR	ZEN、α/β-ZOL、α/β-ZAL	8.0/40	207	[20]
ZHD607 (KIW70607)	Phialophora americana	ZEN	8.0/35	4 940±28.06	[21]
ZHD11D (XM_018146622)	Phialophora attinorum	ZEN	8.0/35	304.7	[22]
ZHD11A (NR)	P. macrospora	ZEN	8.0/40	220	[15]
ZHD-P (KF515221)	Trichoderma aggressivum	ZEN	7.5~9.0/45	191	[23]
ZHRnZ (GAP90066.2)	R. necatrix	ZEN、α/β-ZOL、α-ZAL	9.0/45	NR	[16]
ZENY (CP137687)	Bacillus subtilis YT-4	ZEN	8.0/37	1 060±0.06	[24]
ZenR (OR497749)	Rhodococcus erythropolis HQ	ZEN	8.0~9.0/55	20.04	[25]
ZENG (ALI16790.1)	Gliocladium roseum	ZEN、α-ZOL、α-ZAL	7.0/38	315	[26]
CbZHD (XP_016613277.1)	Cladophialophora bantiana	ZEN	8.0/35	219	[27]

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