玉米赤霉烯酮对后备母猪子宫和卵巢抗氧化和炎症指标及相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2020.07.016

摘要/Abstract

摘要： 试验旨在研究玉米赤霉烯酮（zearalenone，ZEA）对后备母猪子宫和卵巢抗氧化和炎症指标及相关基因表达的影响。选择胎次和体重（（23.20±0.68）kg）相近的长×大二元后备母猪48头，随机分为4组，每组设12个重复，每个重复1头母猪。对照组（CON组）饲喂基础饲粮，试验组（T1、T2、T3组）饲喂在基础饲粮中分别添加200、800、1600 μg·kg^-1ZEA的试验饲粮。预试期7 d，正试期40 d。结果如下：1）与CON组相比，T3组血清T-AOC和SOD活性显著降低（P<0.05），MDA水平显著升高（P<0.05）。2）与CON组相比，子宫组织中，T2和T3组T-AOC活性显著降低（P<0.05），T3组SOD活性极显著降低（P<0.01），卵巢组织中，T3组T-AOC活性、T2组GSH-Px和SOD活性显著降低（P<0.05），T3组MDA水平显著升高（P<0.05）。3）与CON组相比，T2组血清TNF-α和IL-4水平显著升高（P<0.05），T1~T3组血清IL-10水平显著降低（P<0.05）。4）与CON组相比，子宫组织中，T2组IL-1β水平显著升高（P<0.05），T1和T3组IL-10水平显著降低（P<0.05）。卵巢组织中，T2组TNF-α水平显著升高（P<0.05），T1~T3组IL-4水平显著升高（P<0.05）。5）子宫组织中，T3组Cu/Zn-SOD mRNA的相对表达显著低于CON组（P<0.05），T2组IL-1β mRNA的相对表达显著高于CON组（P<0.05）。6）卵巢组织中，T3组GSH-Px mRNA的相对表达显著低于CON组（P<0.05），T2组IL-1β mRNA的相对表达显著高于CON组（P<0.05）。综上所述，ZEA能通过抑制SOD和GSH-Px的表达与合成，降低后备母猪子宫和卵巢的抗氧化性能，并引起氧化应激。ZEA能通过促进TNF-α和IL-1β表达与合成引起子宫和卵巢的炎症反应，抑制IL-10的表达与合成从而抑制两组织的抗炎反应。

关键词: 玉米赤霉烯酮, 后备母猪, 子宫, 卵巢, 抗氧化, 炎症

Abstract: This study was designed to investigate the effects of zearalenone (ZEA) on the antioxidative and inflammatory indexes and related genes expression of uterus and ovary in prepubertal gilts. A total of 48 Landrace×Yorkshire prepubertal gilts with similar birth parity, breeds and body weight ((23.20±0.68) kg)were randomly divided into 4 groups(12 replicates per group, 1 gilt per replicate). Prepubertal gilts in control group(CON group)were fed a basal diet and those in experimental groups(T1, T2 and T3 groups) were fed test diets supplemented with 200, 800 and 1 600 μg·kg^-1 ZEA in the basal diet, respectively. Pretest lasted for 7 d, and formal test lasted for 40 d. The results showed as follows:1)The serum T-AOC and SOD activity of prepubertal gilts in the T3 group were significantly lower than those in the CON group (P<0.05), and the MDA level was significantly higher than that in the CON group(P<0.05). 2)In the uterine tissues of prepubertal gilts in each group, T-AOC activity in group T2 and T3 significantly decreased than those in the CON group (P<0.05), and the SOD activity in the group T3 extremely significantly decreased than that in the CON group (P<0.01). In the ovarian tissues of prepubertal gilts in each group, the activity of T-AOC in the T3 group and the activity of GSH-Px and SOD in the T2 group were significantly lower than those in the CON group(P<0.05), and MDA level in the T3 group was significantly higher than that in the CON group(P<0.05). 3)The serum TNF-α and IL-4 levels of prepubertal gilts in the T2 group were significantly higher than those in the CON group(P<0.05)and IL-10 levels of prepubertal gilts in T1, T2 and T3 groups were significantly lower than those in the CON group(P<0.05). 4)In the uterine tissues of prepubertal gilts in each group, IL-1β level in the T2 group was significantly higher than that in the CON group(P<0.05), and IL-10 levels in T1 and T3 groups were significantly lower than those in the CON group(P<0.05). In the ovarian tissues of prepubertal gilts in each group, level of TNF-α in the T2 group was significantly higher than that in the CON group(P<0.05), and levels of IL-4 in T1, T2 and T3 groups were significantly higher than those in the CON group(P<0.05). 5)In the uterine tissues of prepubertal gilts in each group, the relative expression of Cu/Z_n-SOD mRNA in the T3 group was significantly lower than that in the CON group(P<0.05), and the relative expression of IL-1β mRNA in the T2 group was significantly higher than that in the CON group(P<0.05). 6)In the ovarian tissues of prepubertal gilts in each group, the relative expressions of GSH-Px mRNA in the T3 group was significantly lower than that in the CON group(P<0.05), and the relative expressions of IL-1β mRNA in the T2 group was significantly higher than that in the CON group(P<0.05). In conclusion, by inhibiting the expression and synthesis of SOD and GSH-Px, ZEA can reduce the antioxidant performance of uterus and ovary of prepubertalgilts and cause oxidative stress. ZEA can also cause inflammatory responses in the uterus and ovary of prepubertal gilts by promoting the expression and synthesis of TNF-α and IL-1β. Inhibition of IL-10 expression and synthesis inhibits the inflammatory response of the uterus and ovary.

Key words: zearalenone, prepubertal gilts, uterus, ovary, antioxidant, inflammation

中图分类号:

S828
S859.87

吴峰洋, 崔嘉, 杨新宇, 陈宝江. 玉米赤霉烯酮对后备母猪子宫和卵巢抗氧化和炎症指标及相关基因表达的影响[J]. 畜牧兽医学报, 2020, 51(7): 1637-1645.

WU Fengyang, CUI Jia, YANG Xinyu, CHEN Baojiang. Effect of Zearalenone on Uterine and Ovarian Antioxidant and Inflammatory Indexes and Related Gene Expression in Prepubertal Gilts[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1637-1645.

参考文献 26

[1]	ZHOU M,YANG L J,SHAO M H,et al.Effects of Zearalenone exposure on the TGF-β1/Smad3 signaling pathway and the expression of proliferation or apoptosis related genes of post-weaning gilts[J].Toxins (Basel),2018,10(2):E49.
[2]	ZHANG F L,LI N,WANG H,et al.Zearalenone Exposure induces the apoptosis of porcine Granulosa cells and changes long noncoding RNA expression to promote Antiapoptosis by activating the JAK2-STAT3 pathway[J].J Agric Food Chem,2019,67(43):12117-12128.
[3]	ZINEDINE A,SORIANO J M,MOLTÓ J C,et al.Review on the toxicity,occurrence,metabolism,detoxification,regulations and intake of zearalenone:an oestrogenic mycotoxin[J].Food Chem Toxicol,2007,45(1):1-18.
[4]	CHEN X L,XIANG L,JIA G,et al.Effects of dietary leucine on antioxidant activity and expression of antioxidant and mitochondrial-related genes in Longissimus dorsi muscle and liver of piglets[J].Anim Sci J,2019,90(8):990-998.
[5]	REDDY K E,SONG J,LEE H J,et al.Effects of High Levels of Deoxynivalenol and Zearalenone on Growth Performance,and Hematological and Immunological Parameters in Pigs[J].Toxins (Basel),2018,10(3):114.
[6]	陈祥兴,张崇玉,黄丽波,等.镰刀菌毒素对断奶仔猪肠道IL-1β和IL-6分布和表达的影响[J].畜牧兽医学报,2016,47(10):2126-2135.CHEN X X,ZHANG C Y,HUANG L B,et al.Effects of Fusarium toxins on IL-1β and IL-6 in post-weaning piglets[J].Acta Veterinaria et Zootechnica Sinica,2016,47(10):2126-2135.(in Chinese)
[7]	牛群升,杨维仁,黄丽波,等.镰刀菌毒素对断奶小母猪阴户、生殖器官指数、子宫雌激素受体分布和表达的影响[J].动物营养学报,2016,28(5):1525-1533.NIU Q S,YANG W R,HUANG L B,et al.Effects of Fusarium toxins on vulva,reproductive organ index,distribution and expression of estrogen receptors inuterus of weaning gilts[J].Chinese Journal of Animal Nutrition,2016,28(5):1525-1533.(in Chinese)
[8]	杨立杰,王淑静,杨维仁,等.玉米赤霉烯酮对断奶小母猪生产性能、血清抗氧化功能和免疫功能的影响[J].动物营养学报,2017,29(8):2843-2850.YANG L J,WANG S J,YANG W R,et al.Effects of Zearalenone on production performance,serum antioxidant capacity and immune function of weaning gilts[J].Chinese Journal of Animal Nutrition,2017,29(8):2843-2850.(in Chinese)
[9]	SHI B M,SU Y,CHANG S Y,et al.Vitamin C Protects piglet liver against Zearalenone-induced oxidative Stress by modulating expression of nuclear receptors PXR and CAR and their target genes[J].Food Funct,2017,8(10):3675-3687.
[10]	QIN X S,CAO M J,LAI F N,et al.Oxidative stress induced by Zearalenone in Porcine Granulosa cells and its rescue by Curcumin in vitro[J].PLoS One,2015,10(6):e0127551.
[11]	ZHENG W L,FENG N N,WANG Y,et al.Effects of Zearalenone and its derivatives on the synthesis and secretion of mammalian sex steroid hormones:a review[J].Food Chem Toxicol,2019,126:262-276.
[12]	ZHENG W L,PAN S Y,WANG G G,et al.Zearalenone impairs the male reproductive system functions via inducing structural and functional alterations of sertoli cells[J].Environ Toxicol Pharmacol,2016,42:146-155.
[13]	ZHENG W L,WANG B J,LI X,et al.Zearalenone promotes cell proliferation or causes cell death[J].Toxins (Basel),2018,10(5):184.
[14]	TATAY E,ESPÍN S,GARCÍA-FERNÁNDEZ A J,et al.Oxidative damage and disturbance of antioxidant capacity by zearalenone and its metabolites in human cells[J].Toxicol Vitro,2017,45:334-339.
[15]	姜招峰,杨翰仪.氧自由基对CAT、SOD和GPX的氧化修饰研究[J].北京联合大学学报:自然科学版,2003,17(3):12-17.JIANG Z F,YANG H Y.Effects of oxygen free radicals on functions and structures of CAT,SODand GPX in vitro[J].Journal of Beijing Union University:Natural Sciences,2003,17(3):12-17.(in Chinese)
[16]	JEE Y,NOH E M,CHO E S,et al.Involvement of the Fas and Fas ligand in testicular germ cell apoptosis by zearalenone in rat[J].J Vet Sci,2010,11(2):115-119.
[17]	ZHENG W L,WANGB J,WANG L,et al.ROS-mediated cell cycle arrest and apoptosis induced by Zearalenone in mouse sertoli cells via ER stress and the ATP/AMPK pathway[J].Toxins (Basel),2018,10(1):24.
[18]	PFEIFFER E,HILDEBRAND A,DAMM G,et al.Aromatic hydroxylation is a major metabolic pathway of the mycotoxin zearalenone in vitro[J].Mol Nutr Food Res,2009,53(9):1123-1133.
[19]	MARIN D E,PISTOL G C,NEAGOE I V,et al.Effects of zearalenone on oxidative stress and inflammation in weanling piglets[J].Food Chem Toxicol,2013,58:408-415.
[20]	DEL FABBRO L,JESSE C R,DE GOMES M G,et al.The flavonoid chrysin protects against zearalenone induced reproductive toxicity in male mice[J].Toxicon,2019,165:13-21.
[21]	GILL R,TSUNG A,BILLIAR T.Linking oxidative stress to inflammation:toll-like receptors[J].Free Radic Biol Med,2010,48(9):1121-1132.
[22]	俞卓伟,保志军,阮清伟,等.氧化应激-炎症-衰老及其与ApoE基因相关性研究进展[J].生理学报,2013,65(3):338-346.YU Z W,BAO Z J,RUAN Q W,et al.Oxi-inflamm-aging and its association with the polymorphism of ApoE genes[J].Acta Physiologica Sinica,2013,65(3):338-346.(in Chinese)
[23]	FAN W T,LV Y N,REN S,et al.Zearalenone (ZEA)-induced intestinal inflammation is mediated by the NLRP3 inflammasome[J].Chemosphere,2018,190:272-279.
[24]	PISTOL G C,BRAICU C,MOTIU M,et al.ZearalenoneMycotoxin affects immune mediators,MAPK Signalling molecules,nuclear receptors and genome-wide gene expression in pig spleen[J].PLoS One,2015,10(5):e0127503.
[25]	OBREMSKI K.The effect of in vivo exposure to zearalenone on cytokine secretion by Th1 and Th2 lymphocytes in porcine Peyer's patches after in vitro stimulation with LPS[J].Pol J Vet Sci,2014,17(4):625-632.
[26]	PISTOL G C,GRAS M A,MARIN D E,et al.Natural feed contaminant zearalenone decreases the expressions of important pro-and anti-inflammatory mediators and mitogen-activated protein kinase/NF-κB signalling molecules in pigs[J].Br J Nutr,2014,111(3):452-464.