兔源A型多杀性巴氏杆菌Pm3和Pm6的致病性和基因组特征分析

doi:10.11843/j.issn.0366-6964.2025.05.031

摘要/Abstract

摘要：

旨在探究多杀性巴氏杆菌致病性的分子基础。本试验测定了兔源A型多杀性巴氏杆菌Pm3株和Pm6株的致病性，并进一步对其进行全基因组测序和比较基因组学分析。攻毒试验结果显示，Pm3和Pm6滴鼻攻毒后不仅能使试验兔产生严重的化脓性胸膜肺炎，还能导致试验兔的高死亡率。比较基因组学分析结果显示，Pm3和Pm6的基因组都比兔源低毒力多杀性巴氏杆菌s4株和AH09株的大。与s4和AH09相比，在Pm3和Pm6的基因组中鉴定到约120个具有重要生物学功能的特有基因，且有约100个这些特有基因也存在于兔源强毒力多杀性巴氏杆菌的基因组中。此外，不同动物源多杀性巴氏杆菌基因L3型脂多糖核心寡糖外核合成酶基因natC和gatF的序列存在多态性，但兔源菌株(除SD11、CIRMBP-0873和CIRMBP-0884外)的这两个基因序列却完全一致，表现出一定的宿主特异性。Pm3和Pm6均为强毒力菌株。在Pm3和Pm6基因组中鉴定到的存在于兔源强毒力菌株基因组中但不存在于兔源低毒力菌株基因组中的基因可能在Pm3和Pm6的致病过程中发挥重要作用。

关键词: 多杀性巴氏杆菌, 致病性, 全基因组测序, 比较基因组学

Abstract:

This study aimed to determine the molecular basis of the pathogenicity of Pasteurella multocida(P. multocida). The pathogenicity and whole genomic sequences of 2 rabbit sourced serogroup A P. multocida isolates (Pm3 and Pm6) were determined, and then comparative genomics analysis between the 2 isolates and other P. multocida strains was performed. The intranasal inoculation of Pm3 and Pm6 caused severe pathologic lesions and high mortality in inoculated rabbits. By comparison with the low-virulent rabbit-sourced P. multocida strains s4 and AH09, about 120 specific functional genes were identified in the genomes of Pm3 and Pm6, and about 100 of these specific functional genes could also be detected in the genomes of high-virulent rabbit-sourced P. multocida strains. Moreover, different nucleotide polymorphism profiles were identified in the lipopolysaccharide (LPS) outer core biosynthetic genes natC and gatF among the P. multocida strains of L3 genotype isolated from different hosts. Nevertheless, the sequences of the natC and gatF were identical among the rabbit-sourced P. multocida strains of LPS L3 genotype (except for SD11, CIRMBP-0873 and CIRMBP-0884), which suggested that the potential species specificity of the natC and gatF. The Pm3 and Pm6 were highly pathogenic for rabbits, which indicated that the 2 isolates were high-virulent strains. The specific functional genes that identified in the genomes of Pm3, Pm6 and the previously reported high-virulent rabbit-sourced P. multocida strains but not in the genomes of low-virulent rabbit-sourced P. multocida strains s4 and AH09 might contribute to the pathogenicity of the Pm3 and Pm6.

Key words: Pasteurella multocida, pathogenicity, whole genome sequencing, comparative genomics

中图分类号:

S852.61

王锦祥, 苏进博, 付环茹, 孙世坤, 高承芳, 陈冬金, 桑雷, 谢喜平. 兔源A型多杀性巴氏杆菌Pm3和Pm6的致病性和基因组特征分析[J]. 畜牧兽医学报, 2025, 56(5): 2340-2352.

WANG Jinxiang, SU Jinbo, FU Huanru, SUN Shikun, GAO Chengfang, CHEN Dongjin, SANG Lei, XIE Xiping. Pathogenicity and Genomic Features of Rabbit Sourced Serogroup A Pasteurella multocida Isolates Pm3 and Pm6[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(5): 2340-2352.

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组别 Group	试验兔数量/只 No. of rabbit	攻毒菌株 Infection strain	攻毒剂量^a/CFU Infection dose	攻毒方式^b Infection rout
Pm3攻毒组 Pm3 infected group	6	Pm3	6.0×10⁴	滴鼻 Intranasal inoculation
Pm6攻毒组 Pm6 infected group	6	Pm6	6.0×10⁴	滴鼻 Intranasal inoculation
对照组 Control group	6	生理盐水 Normal saline	100 μL	滴鼻 Intranasal inoculation

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