赛鸽源大肠杆菌耐药性检测及多重耐药菌株的全基因组测序分析

doi:10.11843/j.issn.0366-6964.2025.05.034

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (5): 2372-2382.doi: 10.11843/j.issn.0366-6964.2025.05.034

赛鸽源大肠杆菌耐药性检测及多重耐药菌株的全基因组测序分析

石金川¹(), 孙淼¹, 孟令浩¹, 王永强⁴, 耿超¹, 齐朝鲁蒙⁵, 陈亨利⁶, 王梓¹^,²^,³^,*(), 刘锴¹^,²^,³^,*()

1. 内蒙古民族大学动物科技学院, 通辽 028000
2. 内蒙古自治区肉牛疾病防控工程技术研究中心, 通辽 028000
3. 内蒙古自治区肉牛产业学院, 通辽 028000
4. 扎兰屯职业学院, 呼伦贝尔 162650
5. 内蒙古民族大学医学院, 通辽 028000
6. 通辽市农牧业发展中心, 通辽 028000

收稿日期:2024-07-05 出版日期:2025-05-01 发布日期:2025-05-27
通讯作者: 王梓,刘锴 E-mail:571444290@qq.com;liukai721026@163.com;wangzi@imun.edu.cn
作者简介:石金川(2000-)，男，蒙古族，内蒙古通辽人，硕士生，主要从事兽医微生物学及兽医免疫学研究，E-mail: 571444290@qq.com
基金资助:
内蒙古自治区中央引导地方发展基金项目(2022ZY0149);内蒙古锡林郭勒盟科技计划项目(202213)；内蒙古自治区高校基本科研业务费项目(GXKY22082；GXKY22097)

Detection of Antibiotic Resistance in Escherichia coli Strains Sourced from Racing Pigeons and Whole-genome Sequencing Analysis of Multidrug-resistant Strains

SHI Jinchuan¹(), SUN Miao¹, MENG Linghao¹, WANG Yongqiang⁴, GENG Chao¹, QI Chaolumeng⁵, CHEN Hengli⁶, WANG Zi¹^,²^,³^,*(), LIU Kai¹^,²^,³^,*()

1. School of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China
2. Inner Mongolia Autonomous Region Beef Cattle Disease Prevention and Control Engineering Technology Research Center, Tongliao 028000, China
3. Beef Cattle Industry School of Inner Mongolia Autonomous Region, Tongliao 028000, China
4. Zhalantun Vocational College, Hulun Buir 162650, China
5. Medical school, Inner Mongolia University for Nationalities, Tongliao 028000, China
6. Tongliao Agriculture and Animal Husbandry Development Center, Tongliao 028000, China

Received:2024-07-05 Online:2025-05-01 Published:2025-05-27
Contact: WANG Zi, LIU Kai E-mail:571444290@qq.com;liukai721026@163.com;wangzi@imun.edu.cn

摘要/Abstract

摘要：

赛鸽养殖中，普遍存在抗生素滥用现象，造成耐药菌株增加，多重耐药性加剧。本研究旨在了解通辽市赛鸽源大肠杆菌耐药性及耐药基因流行情况，为赛鸽源大肠杆菌病临床治疗提供参考。使用大肠杆菌鉴别培养基，16S rRNA测序等方法对通辽地区送检腹泻赛鸽粪便病原菌进行分离纯化。通过药敏试验及耐药基因的PCR检测了解分离菌株的耐药性及耐药基因分布情况。对两株耐药性较强的大肠杆菌进行全基因组测序分析。结果显示：分离得到8株赛鸽源大肠杆菌，均为多重耐药菌株，其中卡那霉素和链霉素耐药率均为100%，全部菌株对头孢哌酮/舒巴坦敏感。E-ge1，E-ge2耐药性严重，对两株菌进行全基因组测序分析，获得完整基因组序列，两株菌基因组长度分别为4 680 817 bp与4 691 799 bp，分别编码4 888与5 070个基因，两菌株均携带3个质粒。E-ge1共携带81个耐药基因，E-ge2携带74个耐药基因。通辽地区赛鸽源大肠杆菌耐药形势严峻，菌株携带大量耐药基因，应优化用药策略，控制耐药性的加剧和耐药基因的传播。

关键词: 赛鸽, 大肠杆菌, 耐药性, 耐药基因, 全基因组测序

Abstract:

There is widespread misuse of antibiotics in pigeon breeding, leading to an increase in antibiotic-resistant strains and exacerbating multidrug resistance. The present study aimed to understand the prevalence of antibiotic resistance and resistance genes in Escherichia coli sourced from racing pigeons in Tongliao City, and to provide reference information for clinical treatment of E. coli infections in racing pigeons. Using E. coli selective culture media and methods such as 16S rRNA sequencing, pathogenic bacteria from diarrheic racing pigeons in Tongliao were isolated and purified. Drug susceptibility testing and PCR detection of resistance genes were conducted to assess the resistance profiles and distribution of resistance genes in the isolated strains. Whole genome sequencing analysis was performed on two highly resistant E. coli strains. Eight strains of E.coli sourced from racing pigeons were isolated, and all of them exhibiting multidrug resistance. The resistance rates to kanamycin and streptomycin were both 100%, while all strains were sensitive to ceftriaxone-sulbactam. Strains E-ge1 and E-ge2 showed severe resistance profiles. Whole genome sequencing analysis of these two strains revealed complete genome sequences, with lengths of 4 680 817 bp and 4 691 799 bp respectively. They encoded 4 888 and 5 070 genes respectively, and both strains carried three plasmids.E-ge1 carries a total of 81 antibiotic resistance genes, whereas E-ge2 harbors 74 such genes. The situation of antibiotic resistance in E.coli sourced from racing pigeons in Tongliao is severe, with strains carrying a large number of resistance genes. It is imperative to optimize treatment strategies to control the exacerbation of resistance and the spread of resistance genes.

Key words: racing pigeons, Escherichia coli, antibiotic resistance, resistance genes, whole-genome sequencing

中图分类号:

S852.612

石金川, 孙淼, 孟令浩, 王永强, 耿超, 齐朝鲁蒙, 陈亨利, 王梓, 刘锴. 赛鸽源大肠杆菌耐药性检测及多重耐药菌株的全基因组测序分析[J]. 畜牧兽医学报, 2025, 56(5): 2372-2382.

SHI Jinchuan, SUN Miao, MENG Linghao, WANG Yongqiang, GENG Chao, QI Chaolumeng, CHEN Hengli, WANG Zi, LIU Kai. Detection of Antibiotic Resistance in Escherichia coli Strains Sourced from Racing Pigeons and Whole-genome Sequencing Analysis of Multidrug-resistant Strains[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(5): 2372-2382.

图/表 11

表 1

图 1

表 2

表 3

图 2

表 4

图 3

图 4

图 5

图 6

表 5

参考文献 28

1	LAXMINARAYAN R , SRIDHAR D , BLASER M , et al. Achieving global targets for antimicrobial resistance[J]. Science, 2016, 353 (6302): 874- 875. doi: 10.1126/science.aaf9286
2	陈霞. 猪源大肠杆菌五种毒力因子单重及多重荧光定量PCR方法的建立及初步应用[D]. 武汉: 华中农业大学, 2022.
	CHEN Xia. Establishment and preliminary application of single and multiplex qPCR methods for detection of five virulence factors of porcine E. coli[D]. Wuhan: Huazhong Agricultural University, 2022. (in Chinese)
3	WANG A Q , HU C M . Antimicrobial resistance analysis of Escherichia coli isolated from pigeons in Qingdao, Shandong Province, China[J]. Genes (Basel), 2022, 13 (9): 1510. doi: 10.3390/genes13091510
4	VON TÖNNEMANN PILATI G , CADAMURO R D , FILHO V B , et al. Bacteriophage-associated antimicrobial resistance genes in avian pathogenic Escherichia coli isolated from Brazilian poultry[J]. Viruses, 2023, 15 (7): 1485. doi: 10.3390/v15071485
5	ABERKANE C , MESSAÏ A , MESSAÏ C R , et al. Antimicrobial resistance pattern of avian pathogenic Escherichia coli with detection of extended-spectrum β-lactamase-producing isolates in broilers in east Algeria[J]. Vet World, 2023, 16 (3): 449- 454.
6	GHANBARPOUR R , AFLATOONIAN M R , ASKARI A , et al. Domestic and game pigeons as reservoirs for Escherichia coli harbouring antimicrobial resistance genes[J]. J Glob Antimicrob Resist, 2020, 22, 571- 577. doi: 10.1016/j.jgar.2020.02.015
7	CHROBAK-CHMIEL D , KWIECIE? E , GOLKE A , et al. Pigeons as carriers of clinically relevant multidrug-resistant pathogens-a clinical case report and literature review[J]. Front Vet Sci, 2021, 8, 664226. doi: 10.3389/fvets.2021.664226
8	LUO S S , LIAO C Y , PENG J J , et al. Resistance and virulence gene analysis and molecular typing of Escherichia coli from duck farms in Zhanjiang, China[J]. Front Cell Infect Microbiol, 2023, 13, 1202013. doi: 10.3389/fcimb.2023.1202013
9	王梓, 孙淼, 王永强, 等. 通辽部分地区鸭源大肠杆菌耐药性检测及多重耐药菌株YA-1全基因组测序分析[J]. 中国畜牧兽医, 2024, 51 (6): 2585- 2596.
	WANG Z , SUN M , WANG Y Q , et al. Detection of drug resistance of Escherichia coli from ducks in some areas of Tongliao and whole genome sequencing analysis of multi-drug resistant strain YA-1[J]. China Animal Husbandry & Veterinary Medicine, 2024, 51 (6): 2585- 2596.
10	王永强, 李偲, 耿超, 等. 通辽地区犊牛腹泻大肠杆菌耐药性检测及一株多重耐药菌全基因组测序分析[J]. 微生物学通报, 2022, 49 (12): 4964- 4977.
	WANG Y Q , LI C , GENG C , et al. Drug resistance of Escherichia coli strains causing calf diarrhea in Tongliao and whole-genome sequencing of a multi-drug resistant strain[J]. Microbiology China, 2022, 49 (12): 4964- 4977.
11	HUMPHRIES R , BOBENCHIK A M , HINDLER J A , et al. Overview of changes to the clinical and laboratory standards institute performance standards for antimicrobial susceptibility testing, M100, 31st edition[J]. J Clin Microbiol, 2021, 59 (12): e0021321. doi: 10.1128/JCM.00213-21
12	郭双. 通辽地区犊牛腹泻大肠杆菌耐药性检测及一株多重耐药菌株全基因组测序分析[D]. 通辽: 内蒙古民族大学, 2022.
	GUO S. Detection of drug resistance of Escherichia coli in calves with diarrhea in Tongliao region and whole genome sequencing analysis of a multi-drug resistant strain[D]. Tongliao: Inner Mongolia Minzu University, 2022. (in Chinese)
13	KOREN S , WALENZ B P , BERLIN K , et al. Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation[J]. Genome Res, 2017, 27 (5): 722- 736. doi: 10.1101/gr.215087.116
14	HYATT D , CHEN G L , LOCASCIO P F , et al. Prodigal: prokaryotic gene recognition and translation initiation site identification[J]. BMC Bioinf, 2010, 11 (1): 119. doi: 10.1186/1471-2105-11-119
15	BERTELLI C, BRINKMAN F S L. Improved genomic island predictions with IslandPath-DIMOB[J]. Bioinformatics, 34(13): 2161-2167.
16	FU L M , NIU B F , ZHU Z W , et al. CD-HIT: accelerated for clustering the next-generation sequencing data[J]. Bioinformatics, 2012, 28 (23): 3150- 3152. doi: 10.1093/bioinformatics/bts565
17	ASHBURNER M , BALL C A , BLAKE J A , et al. Gene ontology: tool for the unification of biology[J]. Nat Genet, 2000, 25 (1): 25- 29. doi: 10.1038/75556
18	KANEHISA M , GOTO S , KAWASHIMA S , et al. The KEGG resource for deciphering the genome[J]. Nucleic Acids Res, 2004, 32 (S1): D277- D280.
19	JIA B F , RAPHENYA A R , ALCOCK B , et al. CARD 2017: expansion and model-centric curation of the comprehensive antibiotic resistance database[J]. Nucleic Acids Res, 2017, 45 (D1): D566- D573. doi: 10.1093/nar/gkw1004
20	AFAYIBO D J A , ZHU H , ZHANG B B , et al. Isolation, molecular characterization, and antibiotic resistance of avian pathogenic Escherichia coli in eastern China[J]. Vet Sci, 2022, 9 (7): 319. doi: 10.3390/vetsci9070319
21	ALCOCK B P , RAPHENYA A R , LAU T T Y , et al. CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database[J]. Nucleic Acids Res, 2020, 48 (D1): D517- D525.
22	刘安娜. 成都赛鸽源大肠埃希菌和肠球菌分离鉴定及对常用抗生素的耐药性分析[D]. 成都: 西南民族大学, 2022.
	LIU A N. Isolation and identification of Escherichia coli and enterococcus faecalis from pigeons in Chengdu and analysis of their resistance to common antibiotics[D]. Chengdu: Southwest Minzu University, 2022. (in Chinese)
23	杨文仪, 肖家伟, 雷连成, 等. 鸽源大肠杆菌O157: 非H7菌株的致病性和耐药性分析[J]. 中国农业大学学报, 2023, 28 (6): 169- 182.
	YANG W Y , XIAO J W , LEI L C , et al. Pathogenicity and drug resistance isolation, identification and biological characteristics of pigeon derived Escherichia coli O157: non H7 strain[J]. Journal of China Agricultural University, 2023, 28 (6): 169- 182.
24	吕敏娜, 孙铭飞, 沈浩铎. 2011~2018年广东鸽源大肠杆菌、沙门氏菌耐药性分析[J]. 畜牧兽医科技信息, 2019 (12): 46- 48.
	LÜ M N , SUN M F , SHEN H D . Analysis of drug resistance of Escherichia coli and Salmonella from pigeon in Guangdong Province from 2011 to 2018[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine, 2019 (12): 46- 48.
25	CAPITA R , CORDERO J , MOLINA-GONZÁLEZ D , et al. Phylogenetic diversity, antimicrobial susceptibility and virulence characteristics of Escherichia coli isolates from pigeon meat[J]. Antibiotics (Basel), 2019, 8 (4): 259. doi: 10.3390/antibiotics8040259
26	GAUBA A , RAHMAN K M . Evaluation of antibiotic resistance mechanisms in gram-negative bacteria[J]. Antibiotics (Basel), 2023, 12 (11): 1590. doi: 10.3390/antibiotics12111590
27	MUKERJI S , GUNASEKERA S , DUNLOP J N , et al. Implications of foraging and interspecies interactions of birds for carriage of Escherichia coli strains resistant to critically important antimicrobials[J]. Appl Environ Microbiol, 2020, 86 (20): e01610- 20.
28	BUJŇÁKOVÁ D , KOCÚREKOVÁ T , KARAHUTOVÁ L . Distribution of virulence-associated genes, antibiotic resistance and phylogenetic groups in Escherichia coli isolated from domestic and racing pigeons[J]. Vet Res Commun, 2023, 47 (3): 1697- 1705. doi: 10.1007/s11259-023-10126-w

目的基因	引物序列(5'→3')	扩增长度/bp
Target gene	Primer sequence	Amplification length
16S rRNA	F: AGAGTTTGATCCTGGCTCAG R: GGTTACCTTGTTACGACTT	1 369
strA	F: ATCGCAGATAGAAGGCAAGGC R: AACTGGCAGGAGGAACAGGA	574
qnrB	F: GATCGTGAAAGCCAGAAAGG R: ACGATGCCTGGTAGTTGTCC	469
tetA	F: GCTACATCCTGCTTGCCTTC R: CATAGATCGCCGTGAAGAGG	210
TEM-1	F: TTTTCGTGTCGCCCTTAT R: GCTCGTCGTTTGGTATGG	512
qnrS	F: ACGACATTCGTCAACTGCAA R: TAAATTGGCACCCTGTAGGC	417
strB	F: GCGTTGCTCCTCTTCTCCAT R: ACCTTTTCCAGCCTCGTTTG	723
bla-CTX	F: CGCTTTGCGATGTGCAG R: ACCGCGATATCGTTGGT	550
qnrD	F: TTTTCGCTAACTAACTCGC R: GAAAGGATAAACAGGCAAAT	984
CTXM-55	F: GCTGGGTAAAGCATTGGG R: TAGGTTGAGGCTGGGTGA	224
AAC(3)-Ⅱa	F: TCACGAACTCGGTCACGC R: CCAAGCATCGGCATCTCA	177
qnrC	F: ATTTCTCACAGGCAAACT R: CTGGAATAACAATCACCC	666
tetR	F: AACAACCCGTAAACTCGC R: GGTGCCTATCTAACATCTCAAT	116
floR	F: CTGAACACGACGCCCGCTAT R: GGACCGCTCCGCAAACAA	751

抗生素 Antibiotic	菌株编号Strain No.								耐药率/% Resistance rate
抗生素 Antibiotic	E-ge1	E-ge2	E-ge3	E-ge4	E-ge5	E-ge6	E-ge7	E-ge8	耐药率/% Resistance rate
恩诺沙星	R(0)	R(0)	R(8)	R(9)	I(13)	I(10)	S(18)	S(26)	50(4/8)
氧氟沙星	R(0)	R(0)	R(0)	R(0)	I(11)	I(12)	I(12)	S(29)	50(4/8)
环丙沙星	R(0)	R(0)	I(11)	I(12)	I(12)	I(12)	I(13)	S(21)	25(2/8)
多黏菌素B	S(16)	R(0)	S(13)	S(12)	S(11)	S(10)	S(11)	S(11)	12.5(1/8)
卡那霉素	R(0)	R(0)	R(0)	R(0)	R(7)	R(0)	R(9)	R(7)	100(8/8)
庆大霉素	R(0)	R(0)	R(0)	R(0)	I(10)	I(10)	I(10)	R(8)	62.5(5/8)
大观霉素	R(0)	R(0)	R(0)	R(9)	I(11)	I(11)	R(7)	I(12)	62.5(5/8)
强力霉素	I(12)	R(0)	I(10)	I(13)	I(10)	R(9)	I(10)	S(18)	25(2/8)
链霉素	R(0)	R(0)	R(9)	R(7)	R(9)	R(8)	R(7)	R(9)	100(8/8)
氯霉素	R(0)	R(0)	R(0)	R(0)	R(0)	R(0)	S(18)	S(20)	75(6/8)
氟苯尼考	R(8)	R(0)	R(0)	R(0)	R(0)	R(0)	S(21)	S(21)	75(6/8)
头孢哌酮/舒巴坦	S(20)	S(17)	S(16)	S(15)	S(23)	S(21)	S(17)	S(19)	0(0/8)
头孢噻肟	R(8)	R(0)	R(0)	R(8)	S(24)	S(22)	S(23)	S(23)	50(4/8)

耐药基因种类	耐药基因	检出率/%
Types of resistance genes	Drug resistance gene	Detection rate
喹诺酮类Quinolones	qnrS	100(8/8)
	qnrB	0(0/8)
	qnrC	0(0/8)
	qnrD	0(0/8)
氯霉素类Chloramphenicols	floR	75(6/8)
四环素类Tetracyclines	tetA	100(8/8)
	tetR	100(8/8)
氨基糖苷类Aminoglycosides	strA	100(8/8)
	strB	100(8/8)
	AAC(3)-Ⅱa	0(0/8)
β-内酰胺类Beta-lactams	Bla-CTX	100(8/8)
	TEM-1	100(8/8)
	CTX-M-55	62.5(5/8)

菌株	基因岛	位置	基因位点	长度/bp
Strains	Genomic-island	Location	Position	Length
E-ge1	Genomic-island-1	染色体(Chromosome)	125575-129913	4 339
E-ge1	Genomic-island-2	染色体(Chromosome)	243652-260821	17 170
E-ge1	Genomic-island-3	染色体(Chromosome)	1138400-1160866	22 467
E-ge1	Genomic-island-4	染色体(Chromosome)	1184477-1189130	4 654
E-ge2	Genomic-island-1	染色体(Chromosome)	408991-427656	18 666
E-ge2	Genomic-island-2	染色体(Chromosome)	908091-929910	21 820
E-ge2	Genomic-island-3	染色体(Chromosome)	1135002-1143203	8 202
E-ge2	Genomic-island-4	染色体(Chromosome)	1857146-1877628	20 483

菌株	质粒	耐药基因
Strains	plasmids	Drug resistance genes
E-ge1	pE-ge1-1	floR、tet(C)、APH(6)-Id、APH(3″)-Ib、sul2、APH(3')-Ⅱa、vgaC、TEM-1、rmtB、TEM-2、 CTX-M-55、APH(3″)-Ib、sul2、APH(3')-Ⅱa、vgaC、TEM-1、rmtB、TEM-2、CTX-M-55
	pE-ge1-2	aadA23、aadA23
	pE-ge1-3	TEM-1、AAC(3)-Ⅱa、tet(C)
E-ge2	pE-ge2-1	aadA23
	pE-ge2-2	TLA-2
	pE-ge2-3	floR、tet(C)、APH(6)-Id、APH(3″)-Ib、sul2、APH(3')-Ⅱa、vgaC、TEM-1、rmtB、TEM-2、 CTX-M-55

赛鸽源大肠杆菌耐药性检测及多重耐药菌株的全基因组测序分析

Detection of Antibiotic Resistance in Escherichia coli Strains Sourced from Racing Pigeons and Whole-genome Sequencing Analysis of Multidrug-resistant Strains

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 28

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王锦祥, 苏进博, 付环茹, 孙世坤, 高承芳, 陈冬金, 桑雷, 谢喜平. 兔源A型多杀性巴氏杆菌Pm3和Pm6的致病性和基因组特征分析[J]. 畜牧兽医学报, 2025, 56(5): 2340-2352.
[2]	梁莉雯, 李军星, 袁秀芳, 余斌, 叶十一, 徐丽华, 苏菲, 刘璨颖. 从油茶籽提取的茶皂素对猪源多重耐药产肠毒素大肠杆菌的体外抑菌效果[J]. 畜牧兽医学报, 2025, 56(5): 2451-2465.
[3]	廖怡雯, 叶景芬, 武绍碧, 陈世雄, 杨婉, 罗雪, 杨琦. 环介导等温扩增技术的发展及其在耐药基因检测中的应用[J]. 畜牧兽医学报, 2025, 56(4): 1621-1631.
[4]	吴俊杰, 吕世明, 龙小霞, 王忠, 王立琦. 中药及活性成分抗耐药菌作用及其机制研究进展[J]. 畜牧兽医学报, 2025, 56(4): 1632-1647.
[5]	邱谦, 桑锐, 王巍, 刘馨蔓, 于明弘, 刘晓童, 于天, 张雪梅. 呼宁散对鸡肺源大肠杆菌抑菌活性及体外抗炎、抗氧化作用研究[J]. 畜牧兽医学报, 2025, 56(4): 1969-1980.
[6]	李案本, 符娜娜, 罗晓平, 李军燕, 刘阳. 捻转血矛线虫的耐药性及其干预途径研究进展[J]. 畜牧兽医学报, 2025, 56(2): 523-533.
[7]	杜清洁, 吴礼平, 张帆, 戴鹏秀, 冯献程, 张欣珂. 牙周炎患犬口腔菌群差异性及卟啉单胞菌耐药性分析[J]. 畜牧兽医学报, 2025, 56(2): 934-942.
[8]	吴平先, 王俊戈, 刁淑琪, 柴捷, 查琳, 郭宗义, 陈红跃, 龙熙. 基于填充测序数据的荣昌猪群体遗传结构和选择信号分析[J]. 畜牧兽医学报, 2025, 56(1): 147-158.
[9]	王浩, 王世玉, 肖金龙, 沈珏, 潘天灵, 张靖松, 肖鹏, 高洪. 猪源大肠杆菌高致病性毒力岛结构基因进化分析及其对TNF/NF-κB通路的影响[J]. 畜牧兽医学报, 2025, 56(1): 392-403.
[10]	王佳宁, 张自强, 王帅帅, 刘玉梅. 兔源大肠杆菌、奇异变形杆菌和球虫混合感染及药物敏感性分析[J]. 畜牧兽医学报, 2024, 55(9): 4204-4212.
[11]	田思瑾, 赵佳琪, 王晓明, 王丽平, 黄金虎. 我国猪链球菌对常用抗菌药物耐药性的Meta分析[J]. 畜牧兽医学报, 2024, 55(7): 3163-3176.
[12]	王玮, 王景松, 郭亚男, 高乐, 王玲玲, 陈灿, 王健霖, 王建东, 马科, 李继东. 宁夏部分地区犊牛腹泻样本大肠杆菌分离菌株的毒力因子检测与分析[J]. 畜牧兽医学报, 2024, 55(7): 3261-3266.
[13]	邹紫莹, 黄安雄, 阮紫涵, 郝海红. 鸡滑液囊支原体对常用抗菌药流行病学临界值的建立[J]. 畜牧兽医学报, 2024, 55(6): 2701-2715.
[14]	罗婷, 韩著, 徐业芬, 蔡林, 索朗斯珠, 徐晋花, 牛家强. 西藏牦牛源牛支原体T10株全基因组测序及其序列分析[J]. 畜牧兽医学报, 2024, 55(5): 2154-2167.
[15]	郑芮, 刘紫石, 张康友, 颜勇, 魏玲, 泽仁翁姆, 丁则德米, 黄建钧, 王利, 魏勇. 花生茎源茉莉炭疽菌的分离鉴定及生物学特性研究[J]. 畜牧兽医学报, 2024, 55(5): 2206-2213.