中国禽传染性支气管炎病毒的遗传演化及时空传播分析

doi:10.11843/j.issn.0366-6964.2023.06.032

摘要/Abstract

摘要： 对从GenBank数据库下载的所有传染性支气管炎病毒(infectious bronchitis virus, IBV)中国分离株之N基因序列进行分析,旨在探索其遗传变异规律和时空传播情况。通过生物信息学软件MEGA 6.0、RDP 4.95、SimPlot 3.5.1、BEAST v1.10.4、jmodeltest 2.1.7、Tracer v1.7.1、TempEst v1.5.1、FigTree v1.4.3以及SpreaD3 v0.9.7等对分离株分别进行系统进化树、基因重组、毒株溯源、种群动态和时空传播分析。系统进化树分析显示,国内IBV毒株分为6种基因型,以LX4型为主;重组分析发现,1株四川分离株N基因存在重组现象;贝叶斯最大分支置信树分析显示,中国IBV最可能起源于20世纪30年代初的辽宁省。贝叶斯谱系地理学分析显示,IBV毒株在国内存在多条传播路径并形成了3个流行中心:东北地区(黑龙江、辽宁和吉林)、华北和华东地区(河北、山东和江苏)以及华南地区(广东、广西),其中,山东最可能成为IBV的毒株来源库。本研究揭示了中国IBV的起源及其传播途径,N基因存在基因重组现象,提示有必要加强分离株N基因的分析,持续开展系统的分子流行病学研究。

关键词: 禽传染性支气管炎病毒, 遗传变异, 基因重组, 起源, 时空传播

Abstract: The aim of this study was to explore the rules of the genetic variation and spatio-temporal transmission of the Chinese IBV isolates, all the sequences of N gene from GenBank database were downloaded. Bioinformatic software including MEGA 6.0, RDP 4.95, SimPlot 3.5.1, BEAST v1.10.4, jmodeltest 2.1.7, Tracer v1.7.1, TempEst v1.5.1, FigTree v1.4.3 and SpreaD3 v0.9.7 were respectively used to analyze the characteristics of phylogenetic tree, recombination events, origin,population dynamics and spatio-temporal transmission of the IBV isolates. The phylogenetic tree analysis showed that a total of six genotypes were identified, and the LX4-type was the predominant genotype. The recombination analysis showed that recombination event was found in N gene in one of isolate from Sichuan province. The maximum clade credibi-lity tree showed that the Chinese IBVs were most likely to be originated in Liaoning province in the early 1930s. Bayesian phylogeographic analyses indicated that multiple transmission routes and three epicenters in China were found, including the Northeastern Region (Heilongjiang, Liaoning, and Jilin), the Northern and Eastern Region (Hebei, Shandong and Jiangsu) and the Southern Region (Guangdong and Guangxi). Shandong has been the source of spreads in China. Our study revealed that the IBVs' origin, transmission routes and a recombination event occurred in N gene in China, suggesting that it is necessary to continue to carry out the molecular epidemiology study and strengthen the analysis of the N gene of IBV.

Key words: infectious bronchitis virus, genetic variation, recombination, origin, spatial transmission

中图分类号:

S851.31

范文胜, 刘思伽, 邱深本, 黄爱芳, 王艳, 刘敏芳, 梅敏敏, 陈新亮, 韦平, 磨美兰. 中国禽传染性支气管炎病毒的遗传演化及时空传播分析[J]. 畜牧兽医学报, 2023, 54(6): 2543-2554.

FAN Wensheng, LIU Sijia, QIU Shenben, HUANG Aifang, WANG Yan, LIU Minfang, MEI Minmin, CHEN Xinliang, WEI Ping, MO Meilan. Genetic Evolution and Spatio-temporal Transmission Analysis of the Chinese Infectious Bronchitis Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2543-2554.

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