1株伪狂犬病病毒的基因变异及其对小鼠的致病性分析

doi:10.11843/j.issn.0366-6964.2023.04.039

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (4): 1766-1770.doi: 10.11843/j.issn.0366-6964.2023.04.039

• 研究简报 • 上一篇

1株伪狂犬病病毒的基因变异及其对小鼠的致病性分析

倪征, 叶伟成, 陈柳, 云涛, 华炯钢, 朱寅初, 张存*

浙江省农业科学院畜牧兽医研究所, 杭州 310021

收稿日期:2022-08-11 出版日期:2023-04-23 发布日期:2023-04-27
通讯作者: 张存,主要从事动物病毒学研究,E-mail:zhangcun@aliyun.com
作者简介:倪征(1979-),女,浙江杭州人,硕士,主要从事动物病毒学研究,E-mail:nizheng79@163.com
基金资助:
浙江省基础公益研究计划（LGN21C180002）

Genetic Variation and Pathogenicity of a Pseudorabies Virus Variant Strain in Mice

NI Zheng, YE Weicheng, CHEN Liu, YUN Tao, HUA Jionggang, ZHU Yinchu, ZHANG Cun*

Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2022-08-11 Online:2023-04-23 Published:2023-04-27

摘要/Abstract

摘要： 本研究旨在了解我国猪伪狂犬病病毒（pseudorabies virus，PRV）流行毒株的分子遗传变异及毒力特点。对1株分离自1个接种了Bartha-K61疫苗的规模猪场的PRV ZJ株进行了主要糖蛋白基因序列（gB、gC和gE）的测定分析及对6周龄BALB/c小鼠的致病性研究。遗传变异分析显示，该毒株与我国2012年后的流行毒株高度同源，具有变异毒株基因特征，但gE基因的510位氨基酸出现了不同于变异株的独特变化S⁵¹⁰G。ZJ株对小鼠具有较高的致病性，攻毒小鼠呈现典型的神经症状，以100 LD₅₀感染小鼠，第5天死亡率达100%。本研究为进一步探索该病毒的毒株变异特点与致病机理奠定基础。

关键词: 伪狂犬病病毒, 变异株, 遗传变异, 致病性

Abstract: This study aimed to understand the molecular genetic variation and virulence characteristics of porcine pseudorabies virus (PRV) in China. The main glycoprotein gene sequences (gB, gC and gE) of PRV ZJ strain isolated from a pig farm inoculated with Bartha-K61 vaccine were determined. and its pathogenicity to 6-week-old mice was studied. Phylogenetic analysis results showed that this strain was highly homologous to epidemic strains in China after 2012, and had the genetic characteristics of the mutant strain. However, there was a unique change of S⁵¹⁰G in the amino acid position 510 of gE gene, which was different from the mutant strain. ZJ strain was highly pathogenic to BALB/c mice, and the challenged mice showed typical neurological symptoms. The mortality rate reached 100% on day 5 after the mice were infected with 100 LD₅₀. This study laid a foundation for further exploration of the variation characteristics and pathogenesis of this virus.

Key words: pseudorabies virus, variantstrain, genetic evolution analysis, pathogenicity

中图分类号:

S852.659.1

倪征, 叶伟成, 陈柳, 云涛, 华炯钢, 朱寅初, 张存. 1株伪狂犬病病毒的基因变异及其对小鼠的致病性分析[J]. 畜牧兽医学报, 2023, 54(4): 1766-1770.

NI Zheng, YE Weicheng, CHEN Liu, YUN Tao, HUA Jionggang, ZHU Yinchu, ZHANG Cun. Genetic Variation and Pathogenicity of a Pseudorabies Virus Variant Strain in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1766-1770.

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1株伪狂犬病病毒的基因变异及其对小鼠的致病性分析

Genetic Variation and Pathogenicity of a Pseudorabies Virus Variant Strain in Mice

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