牦牛源牛凸隆病毒的检测及其基因组特征

doi:10.11843/j.issn.0366-6964.2021.02.019

摘要/Abstract

摘要： 本研究旨在证实青藏高原牦牛中牛凸隆病毒（bovine torovirus，BToV）的存在，并分析其基因组特征。采用RT-PCR方法检测西藏、青海、四川藏区的犊牦牛腹泻粪便中BToV，并扩增基因组。结果从145份样本中检出10份BToV阳性，平均阳性率约为6.9%，其中西藏、青海、四川藏区的阳性率分别约为11.8%、8.9%、3.0%。成功获得1个长为28 314 bp的牦牛源BToV基因组，GC含量为36.31%，与GenBank中已有的5个BToV基因组的核苷酸相似性为82%～97%，与国内BToV基因组SC2的相似性最高且遗传关系最近。本研究中BToV基因组的S基因与GenBank中已有的14个完整BToV-S基因相比，存在8个独特的氨基酸变异，其中，6个位于S1结构域，2个位于S2结构域，且在222—2 473 bp发生区域重组事件。本研究证明BToV在牦牛中的存在，并且地域分布广泛，获得了牦牛源Ⅲ型BToV基因组，报道了BToV的S基因重组事件，有助于进一步了解BToV的分子特征和遗传进化。

关键词: 牦牛, 牛凸隆病毒, 基因组, S基因重组

Abstract: The purpose of this study was to investigate the presence of bovine torovirus (BToV) in yak in Qinghai-Tibetan Plateau and to analyze the characteristics of the BToV genome. An RT-PCR assay was used to detect BToV from clinical samples from Tibet, Qinghai and Sichuan, and the BToV genome was further amplified from the positive sample. Approximate 6.9% (10/145) diarrheic samples were detected as BToV positive, and the positive rate in Tibet, Qinghai and Sichuan was 11.8%, 8.9% and 3.0% respectively. Moreover, a complete BToV genomic sequence was obtained from a positive sample, which was 28 314 nucleotides in length with a GC content of 36.31%. The genome in this study shared 82%-97% nucleotide sequence identity with the 5 BToV genomic sequences in the GenBank database. It shared the highest sequence identity and the closest genetic relationship with the native BToV genome SC2. Compared with all 14 complete BToV-S gene sequences in the GenBank database, the S gene of the BToV genome in this study had 8 unique amino acid variations which include 6 variations in the S1 domain and 2 variations in the S2 domain. Besides, a recombinant event was predicted in the region of 222-2 473 bp of the S gene. In conclusion, this research revealed that BToV could infect yak with wide geographical distribution. Furthermore, a genotype Ⅲ BToV genome was obtained, and a recombinant event was predicted in its S gene. The results of this study would be helpful for a better understanding of BToV's molecular characteristics and genetic evolution.

Key words: yak, bovine torovirus, genome, S gene recombination

中图分类号:

S852.659.6

赵龙, 汤承, 岳华. 牦牛源牛凸隆病毒的检测及其基因组特征[J]. 畜牧兽医学报, 2021, 52(2): 469-477.

ZHAO Long, TANG Cheng, YUE Hua. Detection and Genomic Characteristics of Bovine Torovirus in Yak[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 469-477.

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