宫内发育迟缓对环江香猪哺乳仔猪IGF发育模式的影响

doi:10.11843/j.issn.0366-6964.2023.06.020

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (6): 2414-2420.doi: 10.11843/j.issn.0366-6964.2023.06.020

宫内发育迟缓对环江香猪哺乳仔猪IGF发育模式的影响

耿梅梅¹, 豆梦莹¹, 傅德智¹, 何庆华¹, 孔祥峰^1,2*

1. 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室动物营养生理与代谢过程湖南省重点实验室, 长沙 410125;
2. 中国科学院环江喀斯特农业生态试验站环江香猪研究中心, 环江 547100

收稿日期:2022-09-28 出版日期:2023-06-23 发布日期:2023-06-16
通讯作者: 孔祥峰,主要从事猪的肠道微生态调控研究,E-mail:nnkxf@isa.ac.cn
作者简介:耿梅梅(1982-),女,安徽宿州人,硕士,高级工程师,主要从事分子生物学与动物营养学研究,E-mail:gengmeimei@isa.ac.cn
基金资助:
国家自然科学基金委区域创新发展联合基金重点项目(U20A2056);国家现代农业产业技术体系(CARS-35);湖南省科技领军人才项目(2019RS3022)

Effects of Intrauterine Growth Retardation on Developmental Model of Insulin-Like Growth Factor in Suckling Piglets of Huanjiang Mini-Pig

GENG Meimei¹, DOU Mengying¹, FU Dezhi¹, HE Qinghua¹, KONG Xiangfeng^1,2*

1. Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. Research Center of Huanjiang Mini-pig, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang 547100, China

Received:2022-09-28 Online:2023-06-23 Published:2023-06-16

摘要/Abstract

摘要： 本文旨在探讨宫内发育迟缓(IUGR)发生与胰岛素样生长因子(IGF)发育模式的联系。选用20头体况相近的环江香猪母猪,分娩后从每窝挑出1头体重最大(定义为正常出生重,NBW)仔猪和1头体重最小仔猪(定义为IUGR),分为NBW组和IUGR组,每组20头。分别于0、7、14和21日龄时,从各组随机选取5头仔猪,前腔静脉采血,离心分离血浆,检测IGF-1浓度;采集肝和背最长肌样品,检测IGF-1、IGF-1R、IGFBP-3和IGFBP-5的基因表达水平。结果表明:与NBW仔猪相比,0~21日龄IUGR仔猪体重和血浆IGF-1含量均显著降低(P＜0.05);0~14日龄IUGR仔猪的体重逐渐增加(P<0.05),而21日龄IUGR仔猪的体重与14日龄差异不显著(P＞0.05)。0日龄IUGR仔猪肌肉IGF-1R、IGFBP-3和IGFBP-5、肝IGFBP-5的表达显著高于NBW仔猪(P<0.05),7日龄IUGR仔猪肌肉和肝的IGF-1表达均显著低于NBW仔猪(P<0.05),21日龄IUGR仔猪肌肉IGF-1R的表达水平显著高于NBW仔猪(P<0.05)。7日龄NBW仔猪肝和肌肉IGF-1以及IUGR仔猪肝IGF-1的表达水平显著高于其他日龄(P＜0.05);0、14日龄IUGR仔猪肌肉IGF-1的表达水平显著高于7和21日龄(P＜0.05);7日龄NBW仔猪肝和肌肉IGFBP-5的表达水平显著高于其他日龄(P＜0.05);IUGR仔猪肝IGFBP-3的表达水平在14日龄显著上调,IGFBP-5的表达水平在0日龄显著高于其他日龄(P<0.05)。综上,IUGR可能通过降低哺乳环江香猪IGF-1的表达、调节IGF-1受体及结合蛋白的表达,导致机体内IGF-1含量降低,进而延缓其生长发育。

关键词: 宫内发育迟缓, 环江香猪, 胰岛素样生长因子, 受体, 结合蛋白

Abstract: This study was conducted to investigate the relationship between the occurrence of intrauterine growth retardation (IUGR) and the development pattern of insulin-like growth factor (IGF). Twenty pregnant sows with similar body condition were selected. The largest piglet selected was defined as normal birth weight (NBW) piglet and the smallest piglet was defined as IUGR piglet from each litter after parturition. They were divided into NBW group and IUGR group with 20 piglets per group. At 0, 7, 14, and 21 days of age, 5 piglets were randomly selected from each group, respectively. The blood was collected from the anterior vena cava and the plasma was collected to detect the IGF-1 concentration, and the samples of liver and longissimus dorsi muscle were collected to detect the gene expression levels of IGF-1, IGF-1R,IGFBP-3, and IGFBP-5. The results showed that the body weight and plasma IGF-1 content of IUGR piglets were significantly lower (P＜0.05) during 0 to 21 days of age, compared with the NBW piglets. The body weight of IUGR piglets during 0 to 14 days of age was increased (P＜0.05), but there were no significant difference (P＞0.05) in the body weight of IUGR piglets at 14 and 21 days of age. At 0 day of age, the IUGR piglets had significantly higher (P＜0.05) mRNA expressions of IGF-1R, IGFBP-3 and IGFBP-5 in muscle and IGFBP-5 in liver than NBW piglets; At 7 days of age, the IUGR piglets had significantly lower (P＜0.05) mRNA expression levels of IGF-1 in muscle or liver than NBW piglets; At 21 days of age, the IUGR piglets had significantly higher (P＜0.05) mRNA expression levels of IGF-1R in muscle than NBW piglets. The expression of IGF-1 in liver and muscle of NBW piglets and liver of IUGR piglets at 7 days of age was significantly higher (P＜0.05) than that at other days of age; The mRNA expression level of IGF-1 in muscle of IUGR piglets at 0 and 14 days of age was significantly higher (P＜0.05) than that of IUGR piglets at 7 and 21 days of age. The expression level of IGFBP-5 in liver and muscle of NBW piglets at 7 days of age was significantly higher (P＜0.05) than that at other days of age, and the expression levels of IGFBP-3 at 14 days of age and IGFBP-5 at 0 day of age were significantly up-regulated (P＜0.05) in liver of IUGR piglets. Collectively, these findings suggested that the IUGR may reduce the expression of IGF-1 and regulate the expression of IGF-1 receptor and binding protein in suckling piglets of Huanjiang mini-pig, which may lead to the decrease of IGF-1 content, retard growth and development of suckling piglets.

Key words: intrauterine growth retardation, Huanjiang mini-pig, insulin-like growth factor, receptor, binding protein

中图分类号:

S828.4

耿梅梅, 豆梦莹, 傅德智, 何庆华, 孔祥峰. 宫内发育迟缓对环江香猪哺乳仔猪IGF发育模式的影响[J]. 畜牧兽医学报, 2023, 54(6): 2414-2420.

GENG Meimei, DOU Mengying, FU Dezhi, HE Qinghua, KONG Xiangfeng. Effects of Intrauterine Growth Retardation on Developmental Model of Insulin-Like Growth Factor in Suckling Piglets of Huanjiang Mini-Pig[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2414-2420.

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宫内发育迟缓对环江香猪哺乳仔猪IGF发育模式的影响

Effects of Intrauterine Growth Retardation on Developmental Model of Insulin-Like Growth Factor in Suckling Piglets of Huanjiang Mini-Pig

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