硒对树突状细胞和巨噬细胞功能的调控作用

doi:10.11843/j.issn.0366-6964.2022.09.037

摘要/Abstract

摘要： 旨在探讨硒对树突状细胞（dendritic cells，DCs）和巨噬细胞功能的影响，试验将硒分别与髓源性树突状细胞（bone marrow derived dendritic cells，BMDCs）和腹腔巨噬细胞共同培养后，用流式细胞术检测未成熟BMDCs和巨噬细胞的吞噬活性以及BMDCs上的MHCⅡ、CD86、CD80和CD40的表达量，测定经硒处理后的成熟BMDCs对刺激同种异体淋巴细胞增殖和抗原递呈能力，并用ELISA检测BMDCs和巨噬细胞上清液中细胞因子（IL-12、IL-1β、IFN-γ、IL-6、IL-10、TNF-α、NO）水平的变化。结果显示，当硒的质量浓度在0.18~0.09 mg·L^-1时，BMDCs和巨噬细胞的吞噬活性显著增强（P<0.05），并且BMDCs上的MHCⅡ、CD86和CD80的表达量显著升高（P<0.05），对刺激同种异体淋巴细胞的增殖和抗原递呈能力也显著增强（P<0.05）。此外，在BMDCs的上清中，IFN-γ、IL-12和IL-10的含量显著升高（P<0.05）；在巨噬细胞的上清中，IFN-γ、TNF-α和NO的含量显著升高（P<0.05）。结果表明，一定质量浓度的硒可以增强树突状细胞和腹腔巨噬细胞的功能，值得进一步探究硒对免疫功能的影响。

关键词: 硒, 树突状细胞, 巨噬细胞

Abstract: The aim of this study was to investigate the effects of selenium on dendritic cells (DCs) and macrophages. Selenium was cultured with bone marrow derived dendritic cells (BMDCs) and peritoneal macrophages, respectively. Flow cytometry was used to detect phagocytosis activity of immature BMDCs and macrophage, as well as the phenotype MHCⅡ, CD86, CD80 and CD40 expression on BMDCs, and the ability of BMDCs treated with selenium to stimulate the proliferation and antigen presenting of allogenic lymphocytes. The cytokines (IL-12, IL-1β, IFN-γ, IL-6, IL-10, TNF-α, NO) in the supernatant of BMDCs and macrophages were detected by ELISA. The results showed that when the concentration of selenium was 0.18-0.09 mg·L^-1, the phagocytic activity of BMDCs and macrophages were significantly enhanced(P<0.05), and the expression levels of MHCⅡ, CD86 and CD80 on BMDCs were significantly increased(P<0.05), and the ability to stimulate the proliferation and antigen presentation of allogeneic lymphocytes was also significantly increased (P<0.05). The contents of IFN-γ, IL-12 and IL-10 were significantly increased in the supernatant of BMDCs(P<0.05), while the contents of IFN-γ, TNF-α and NO were significantly increased in the supernatant of macrophages(P<0.05). The results indicated that proper concentration of selenium could enhance the function of DCs and peritoneal macrophages, and it was worth further exploring the effect of selenium on immune function.

Key words: selenium, dendritic cells, macrophages

中图分类号:

S859.1

景丽荣, 张超, 乔杰, 海尼斯格, 杨英, 高珍珍. 硒对树突状细胞和巨噬细胞功能的调控作用[J]. 畜牧兽医学报, 2022, 53(9): 3231-3240.

JING Lirong, ZHANG Chao, QIAO Jie, HAI Nisige, YANG Ying, GAO Zhenzhen. Effects of Selenium on the Function of Dendritic Cells and Macrophages[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3231-3240.

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