纳米硒对犬肾衰中肾组织离子转运体表达及凋亡的影响

doi:10.11843/j.issn.0366-6964.2022.12.033

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (12): 4482-4489.doi: 10.11843/j.issn.0366-6964.2022.12.033

纳米硒对犬肾衰中肾组织离子转运体表达及凋亡的影响

高海航¹, 张嘉宾¹, 张梦迪², 李浩¹, 杨小琦¹, 史菁菁¹, 刘鑫¹, 周东海^1*

1. 华中农业大学动物医学院, 武汉 430070;
2. 塔里木大学动物科学与技术学院, 阿拉尔 843300

收稿日期:2022-05-19 出版日期:2022-12-23 发布日期:2022-12-25
通讯作者: 周东海，主要从事动物中毒与营养代谢病研究，E-mail：bigdefoot@163.com
作者简介:高海航(1997-)，男，陕西宝鸡人，硕士，主要从事动物中毒与营养代谢病研究，E-mail：ghhhzau@163.com
基金资助:
华中农业大学动物医院资助项目(TAH2020)

Effects of Nano-selenium on the Expression and Apoptosis of Renal Tissue Ion Transporters in Dogs with Renal Failure

GAO Haihang¹, ZHANG Jiabin¹, ZHANG Mengdi², LI Hao¹, YANG Xiaoqi¹, SHI Jingjing¹, LIU Xin¹, ZHOU Donghai^1*

1. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China;
2. College of Animal Science and Technology, Tarim University, Alaer 843300, China

Received:2022-05-19 Online:2022-12-23 Published:2022-12-25

摘要/Abstract

摘要： 旨在探究纳米硒作为治疗药物对腺嘌呤诱导急性肾衰犬肾组织离子转运体表达及凋亡的影响。20只体重约4 kg年龄1岁左右的贵宾犬在做基本健康检查并适应性喂养两周后，随机分为空白对照组(Control，饲喂基础日粮30 d)、急性肾衰造模组[Model，15 d基础日粮+15 d腺嘌呤75 mg·(kg·d)^-1]、常规输液治疗组[Infusion，15 d腺嘌呤75 mg·(kg·d)^-1+15 d静注葡萄糖氯化钠60 mL·(kg·d)^-1、呋塞米2~4 mg·kg^-1]、纳米硒治疗组[Nano-Se，15 d腺嘌呤，75 mg·(kg·d)^-1+15 d纳米硒0.5 mg·(kg·d)^-1]、纳米硒预防组[Prevention，15 d纳米硒0.5 mg·(kg·d)^-1+15 d腺嘌呤75 mg·(kg·d)^-1]，每组4只犬。通过血液生化分析，尿常规分析，肾组织石蜡切片免疫组化，Western blot，RT-qPCR检测相关蛋白基因表达水平。结果表明：纳米硒治疗与预防有效降低肾衰特征指标CRE、BUN，恢复肾衰异常尿常规指标尿比重、尿pH，改善肾衰犬机体钙磷代谢；纳米硒治疗对比急性肾衰造模组可有效增加肾组织CaSR、WNKs mRNA与蛋白表达量(P<0.05)，降低NKCC2 mRNA与蛋白表达量(P<0.05)，从而减弱了急性肾衰中过度代偿的重吸收功能；纳米硒治疗较肾衰造模组肾组织促凋亡Bax、Caspase3/9 mRNA与蛋白表达量显著下调(P<0.05)，降低急性肾衰中肾的凋亡效应。

关键词: 犬急性肾衰, 纳米硒, 离子转运, 凋亡

Abstract: The purpose of this study was to investigate the effect of nano-selenium as a therapeutic drug on the expression and apoptosis of renal tissue ion transporters in dogs with acute renal failure induced by adenine. Twenty poodles weighing about 4 kg and aged about 1 year were randomly divided into blank control group (Control, fed with basal diet for 30 days) and acute renal failure model (Model, after basic health examination and adaptive feeding for two weeks, 15 days of basal diet + 15 days of feeding adenine, 75 mg·(kg·d)^-1), conventional infusion treatment group (Infusion, 15 days of feeding adenine, 75 mg·(kg·d)^-1+15 days glucose and sodium chloride injection 60 mL·(kg·d)^-1 and diuretic intravenous furosemide, 2-4 mg·kg^-1), nano-selenium treatment group (Nano-Se, 15 days of feeding adenine, 75 mg·(kg·d)^-1+15 days of feeding nano-selenium, 0.5 mg·(kg·d)^-1), nano-selenium prevention group (Prevention, 15 days of feeding nano-selenium 0.5 mg·(kg·d)^-1+15 days of feeding adenine, 75 mg·(kg·d)^-1), four dogs in each group. The expression levels of related protein genes were detected by blood biochemical analysis, urine routine analysis, immunohistochemistry of renal tissue paraffin sections, Western blot and RT-qPCR. The results show that nano-selenium treatment and prevention can effectively reduce the characteristic indicators of renal failure CRE and BUN, restore the urine specific gravity and urine pH of abnormal urine routine indicators of renal failure, and improve calcium and phosphorus metabolism in dogs with renal failure; compared with acute renal failure model, nano-selenium treatment effectively increased the mRNA and protein expression of CaSR and WNKs in renal tissue (P<0.05), and decreased the mRNA and protein expression of NKCC2 (P<0.05), thereby weakening the overcompensated reabsorption function in acute renal failure. The mRNA and protein expressions of pro-apoptotic Bax and Caspase3/9 in renal tissue of the renal failure model group were significantly down-regulated (P<0.05), which reduced the apoptosis effect of the kidney in acute renal failure.

Key words: acute renal failure in dogs, nano-selenium, ion transport, apoptosis

中图分类号:

S856.59

高海航, 张嘉宾, 张梦迪, 李浩, 杨小琦, 史菁菁, 刘鑫, 周东海. 纳米硒对犬肾衰中肾组织离子转运体表达及凋亡的影响[J]. 畜牧兽医学报, 2022, 53(12): 4482-4489.

GAO Haihang, ZHANG Jiabin, ZHANG Mengdi, LI Hao, YANG Xiaoqi, SHI Jingjing, LIU Xin, ZHOU Donghai. Effects of Nano-selenium on the Expression and Apoptosis of Renal Tissue Ion Transporters in Dogs with Renal Failure[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4482-4489.

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纳米硒对犬肾衰中肾组织离子转运体表达及凋亡的影响

Effects of Nano-selenium on the Expression and Apoptosis of Renal Tissue Ion Transporters in Dogs with Renal Failure

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